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A beginners course in High Parameter Flow Cytometry Data Analysis

TBC

Please contact btf-enquiries@york.ac.uk for further information

This course is designed to train beginners how to undertake high dimensional flow cytometric analysis. This will be a practical based course with the theory and concepts behind dimensionality reduction and clustering in a manner that is relevant and practical to cytometerists. 

No programming experience is required, and so this will be a greater way to remove the barriers to undertaking and supporting deeper flow analysis. It will teach how to use python and flow cytometry analysis software FCS Express (De Novo, Dotmatcs) and will go through the practice as well as the pre-processing tasks required for analysis pipelines.

At the end of the course you should have an understanding of the associated mathematical algorithms, which algorithms to choose, what they illustrate about the data set and how to integrate Python scripts into the FCS Express flow cytometry analysis software. Thanks to our collaborator De Novo Software for helping make this course possible.

Prerequisites to attend

1) Your own laptop with Python installed (instructions will be provided).

2) An understanding the basic principles of flow cytometry compensation, plotting fcs data and conventional gating data analysis.

Course programme

There will be ample opportunity during the evening for further discussion with the course tutors in more informal surroundings.

A preliminary programme is shown below: 

Day one Aims
9.30 am to 10am Reception at Department of Biology, University of York, UK
Coffee/tea will be provided
10am to 10.15am Lecture Introduction: High Parameter, Definitions, Tools, Data, Pipelines and Outputs
Alastair Droop
Karen Hogg
11.30 am to 11.45 am Break - Coffee/tea will be provided 
11.45 am to 1pm  Practical part The beginnings – Python and FCS data
1pm to 1.45pm Lunch – will be provided
1.45pm to 2.45pm Practical – Python – exploring FCS data
2.45pm to 3.30pm Practical – Python – cleaning FCS data
3pm to 3.15pm Break - Coffee / tea will be provided
3.15pm to 3.30pm  Lecture FCS Express – Data input, plot and clean
3.30pm to 4.15pm  Practical FCS Express – Data, key words, plot, clean and explore
4.15pm to 5.15pm Lecture FCS Express: – High parameter data analysis
Day 2 Aims
8.30am Transport from hotel
9am to 9.30am Lecture High parameter data analysis – Dimensionality Reduction and Clustering
Alastair Droop
10am to 11am Practical – FCS Express Dimensionality Reduction and Clustering
11am to 11.30am Break - Coffee / tea will be provided
11.30am to 1.30pm  Practical – High Dimensional data analysis pipeline in Python
Practical – High Dimensional data analysis pipeline using FCS 
Express with Python
1.30 pm to 2.15pm  Lunch – will be provided
2.15pm to 3.30pm Practical - Data analysis in practice – data sets will be provided and you choose how to analyze 
3.30pm to 3.45pm  Break - Coffee / tea will be provided 
3.45pm to 4.15pm  Q&A Data analysis in practice – analysis outputs can be shared and discussed 
4.15pm to 4.55pm Practical - Data analysis in practice – data sets will be provided and you choose how to analyze
5pm to 5.15pm Closing comments

Note: schedule is provisional and subject to change

Course tutors
  • Karen Hogg, Technology Facility, Department of Biology, University of York
  • Alastair Droop, Technology Facility, Department of Biology, University of York
  • Sukhveer Mann, Technology Facility, Department of Biology, University of York
  • Andrea Valle, Dotmatics
Venue and accommodation

The course will use the resources and instrumentation of the Technology Facility, which is part of the Department of Biology.

Accommodation for Tuesday evening is included in the course fees.  Pre and post course accommodation is also available for an additional cost provided sufficient notice is given.  All accommodation will be at the Hotel Indigo in York city centre.

This historic walled city with its medieval guild halls and hostelries offers many attractions for the visitor, including the Jorvik Viking Centre, the National Railway Museum, York Minster, York Castle Museum, Clifford's Tower and the Merchant Adventurers' Hall. York is set in the centre of the Vale of York with the Yorkshire Dales, Moors and Wolds, and stately homes and other attractions are all within easy reach.

Registration

Date TBC

The registration fee covers accommodation on Tuesday night, all lunches and Tuesday evening meal, refreshments, tuition, links or digital content will be available after the course has finished. The special course dinner on the Tuesday evening will be in the historic city of York.

The course investment fee is: £650 Academic Rate | £850 Industrial Rate

As the number of places is limited, early registration is advised. To register for the Flow Cytometry Hands-on Course, please fill in the registration form Reg_Form_Flow_21-22 Oct 25 (PDF , 520kb) and email to btf-enquiries@york.ac.uk

Refund policy

The full registration fees are payable in advance and there is a 10% administrative charge for cancellations received in writing up to 20 working days before the start of the course.  No refunds will be made for cancellations received within 20 working days of the course start date or for the inability to attend the course for whatever reasons. The University is not liable for non-attendance due to travel disruptions, health problems or any other reason that might lead to a delegate not being able to attend the course.  You are strongly advised to ensure you have appropriate insurance.  Substitutions may, of course, be made at any time, providing you inform us in writing.

How to get here

The University of York enjoys a splendid campus location just two miles from the centre of York itself. Getting to York is easy from anywhere in the world. There are excellent rail links which have a short travel time to international airports and we have easy to access major road networks.

The Biology Department is within Campus West at the University.