Protein Production equipment

Our staff provide fully-assisted technical support for the infrequent user and the facility is an excellent equipment resource for the experienced user.

Find out more about the facilities and services we offer at the Protein Production Laboratory, or contact us for more information.

What we offer

A piece of equipment with glass vials in rows

Proteases

The Protein Production labs prepare and supply H3c (PreScission) and TEV protease for general R&D applications. Both proteases are produced in E. coli using TFPP engineered vectors to give near-native HIS-tagged protease.

The expression procedure uses the Im9 solubility tag (which is self-cleaved) to ensure high-level production. The near native proteases are then purified using Ni2+-affinity chromatography and supplied at both high purity and specific activity.

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Our facilities

Process	Instrument	Description
Robotics	HAMILTON	High-spec liquid handling robotics
Fermentation	APPLIKON 70 litre (SIP)	Microbial (steam-in-place)
	APPLIKON 2-7 litre glass (x4)	Microbial / Cell culture
	APPLIKON 20 litre SUBs (x1)	Cell culture
	APPLIKON 0.5 litre MiniBio (x4)	Microbial / Cell culture
Separations	Hollow-fibre separations	Bacteria/insect
	HERAEUS	Continuous flow centrifugation
Incubation	SCIQUIP (x5)	Cooled Microbial Incubators
	INFORS Multitron (x2)	Insect Shaker Incubators
Incubation	THERMO (x2)	Static Cell Culture Incubators
	EPPENDORF	Mammalian Shaker Incubator
Cell Lysis	COLE PALMER	Sonication
	CONSTANT SYSTEMS	Cell Disruptor
Centrifugation	BECKMAN	Process-Scale Centrifugation
	SORVAL Evolution	High-Speed Centrifugation
	BECKMAN	Ultracentrifugation
Purification	BIORAD	NGS Quest
	ÄKTA Pure	Cold cabinet
	ÄKTA Purifier 10	HPLC - Preparative
	ÄKTA Xpress (x3)	Cold cabinet
Microbiology	Class II hood	General microbiology
Tissue Culture	Insect	Baculovirus expression
	Mammalian	HEK293 expression
Imaging	SYNGENE	General Gel Documentation
	THERMO	iBRIGHT FL1000

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