Our staff provide fully-assisted technical support for the infrequent user and the facility is an excellent equipment resource for the experienced user.

Find out more about the facilities and services we offer at the Protein Production Laboratory, or contact us for more information.

What we offer

Contact us

Dr Jared Cartwright

Dr Jared Cartwright

Protein Production Lab Head

jared.cartwright@york.ac.uk
01904 328797
Technology Facility, Department of Biology, University of York, Wentworth Way, York, YO10 5DD UK General enquiries to: btf-pp-group@york.ac.uk

A piece of equipment with glass vials in rows

Proteases

The Protein Production labs prepare and supply H3c (PreScission) and TEV protease for general R&D applications. Both proteases are produced in E. coli using TFPP engineered vectors to give near-native HIS-tagged protease.

The expression procedure uses the Im9 solubility tag (which is self-cleaved) to ensure high-level production. The near native proteases are then purified using Ni2+-affinity chromatography and supplied at both high purity and specific activity.

Contact us for a quotation or supply

Our facilities

ProcessInstrumentDescription
Robotics HAMILTON  High-spec liquid handling robotics
Fermentation APPLIKON 70 litre (SIP)  Microbial (steam-in-place)
  APPLIKON 2-7 litre glass (x4) Microbial / Cell culture
  APPLIKON 20 litre SUBs (x1) Cell culture
  APPLIKON 0.5 litre MiniBio (x4) Microbial / Cell culture
Separations Hollow-fibre separations Bacteria/insect
  HERAEUS Continuous flow centrifugation
Incubation SCIQUIP (x5) Cooled Microbial Incubators
  INFORS Multitron (x2) Insect Shaker Incubators
Incubation THERMO (x2) Static Cell Culture Incubators
  EPPENDORF Mammalian Shaker Incubator
Cell Lysis COLE PALMER Sonication
  CONSTANT SYSTEMS  Cell Disruptor
Centrifugation BECKMAN Process-Scale Centrifugation
  SORVAL Evolution High-Speed Centrifugation
  BECKMAN Ultracentrifugation
Purification BIORAD NGS Quest
  ÄKTA Pure Cold cabinet
  ÄKTA Purifier 10  HPLC - Preparative
  ÄKTA Xpress (x3) Cold cabinet
Microbiology  Class II hood General microbiology
Tissue Culture Insect Baculovirus expression
  Mammalian  HEK293 expression
Imaging SYNGENE General Gel Documentation
  THERMO iBRIGHT FL1000 

Contact us

Dr Jared Cartwright

Dr Jared Cartwright

Protein Production Lab Head

jared.cartwright@york.ac.uk
01904 328797
Technology Facility, Department of Biology, University of York, Wentworth Way, York, YO10 5DD UK General enquiries to: btf-pp-group@york.ac.uk