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Flow cytometry

 

Rapid single cell analysis and sorting.  For both routine testing and further detailed analysis of both single cell centures and mixed cell populations.

Our labs consist of 4 flow cytometers to enable most types of flow cytometry. They are all housed in temperature controlled rooms for sustained stability. The flow cytometers are:

  • Beckman Coulter MoFlo Astrios with 4 laser lines (405, 488, 561 and 633 nm). This system is capable of 6-way (separating and collecting 4 different populations simultaneously), sterile sorting in excess of 80,000 cells per second.
  • 1 Becton Dickinson Fortessa X-20, 2 x Beckman Coulter CyAn ADPs, 1 Apogee A40. We are the UK reference site for the CyAn. This is the most powerful, compact, analyser currently on the market. It is capable of analysing events at tens of thousands of events per second. It has 3 lasers (405, 488 and 633 nm) and 9 colour detectors which enables most types of cell studies to be conducted with ease. As with the MoFlo, the system uses 3 separate pin-holes (one for each laser) to minimise the amount of spectral bleed-through. This can result in near compensation free cell studies with the correct probes.

Contact: Peter O'Toole