Imaging & Cytometry Equipment

EquipmentTechniques
Flow Cytometer
  • Reference site for Beckman Coulter
  • 2 Beckman Coulter CyAn ADP analysers with 3 lasers, 11 detectors
  • Becton Dickinson Fortessa X-20 with 4 lasers and 16 detectors
  • Beckman Coulter MoFlo Astrios sterile cell sorter (80k/s with final concentration of ~1 x 106/s)
  • Apogee flow system A40
  • Rare cell analysis and sorting
  • Simultaneous cell cycle and receptor analysis
  • FRET
  • Small particle analysis
  • Receptor quantification
Confocal microscopy
  • Zeiss LSM 880 with Airyscan on fully motorised invert microscope
  • Zeiss LSM 780 multiphoton with 2NDDs and 2 GaAsP NDDs on fully motorised invert microscope
  • Zeiss LSM 710 on a motorised upright microscope
  • Zeiss LSM 510 META on a motorised invert microscope
  • Live cell imaging chamber
  • Upright and invert microscopes
  • High resolution imaging
  • Spectral analysis and unmixing
  • Multicolour work
  • FRET
  • FRAP
  • Live-cell time lapse
  • Deep tissue imaging
  • Second Harmonic Generation (SHG)

Slide Scanner 

  • Axio Scan.Z1 slide scanner

 

  • Whole slide digitisation
  • Brightfield imaging
  • Multi-channel fluorescence imaging
  • Z-stack imaging for extended depth of field
  • 100 automated slide capacity

Electron microscopy

  • FEI Tecnai 12 BioTWIN G2 transmission electron microscope with SIS CCD camera
  • JEOL JSM-6490LV with Quorum Technologies PP2000 cryosystem
  • Nanometre resolution
  • Immunogold-labelled cryosections and resin sections
  • Particulate specimens
  • Imaging of 3D objects at room temperature and below

Ptychography

  • Phase Focus VL20
  • Phase Focus VL21
  • Phase Focus LiveCyte

 

  • Label-free, high-contrast live cell imaging
  • Cell segmentation
  • Cell cycle analysis
  • Cell mass/volume measurements

Laser capture / microdissection microscopy

  • PALM system with live cell capability
  • Rare cell isolation

Analysis software packages

  • Quorum Technologies Volocity
  • Tissue Gnostics - 
    TissueQuest (fluorescence)
    HistoQuest (brightfield)
  • Beckman Coulter Kaluza
  • FlowJo
  • Summit
  • Plus many other specialist packages
  • Off-line analysis
  • Quantification
  • Co-loaclisation analysis
  • 3-D reconstruction
  • Digital slides from Zeiss AxioScan.Z1 slide scanner can be imported for analysis
  • FACS-like data analysis of images
Peripheral equipment and histology