Gene Expression Technologies

Date tbc: please contact btf-enquiries@york.ac.uk for further information.

Gene expression technologies are central to a wide range of biological research projects, from medical research, such as looking at effects of drug candidates at the molecular level or understanding the cellular changes during cancer development, through to ecological work, such as measuring the changes in complex microbial communities under different environmental stresses. This three-day course covers the dominant technologies that are used to measure gene expression: qPCR and RNASeq. For each technology, the principles of the technique are described followed by the details of the implementation.

The data produced by each methodology is described, and the steps required to go from raw data to reliable measures of differential gene expression are demonstrated. In addition, an outline of the preparation of samples is described, along with the quality control steps necessary to ensure that these technologies can produce reliable results.