UK Biological Imaging Facilities

To search this site use Ctrl+F and enter term

View UK Biological Imaging Facilities in a larger map including Facility listing

Please scroll down the table (alphabetical order) or click the map link for more information about each facility

To add your facility to the list please contact Jo Marrison: jlm2@york.ac.uk

Institution	Equipment
Aberdeen Kevin Mackenzie +44 (0)1224 437447 Miep Helfrich +44 (0)1224 437443 Microscopy and Cellular Imaging Facility Histology and EM Facility Institute of Medical Sciences University of Aberdeen Foresterhill Aberdeen AB25 2Z Access Internal on all instruments External commercial & academic on application DIY or Full service work Expertise Core Facility; Histology, Electron Microscopy, MicroCT	Confocal microscopy Zeiss 700 Laser Scanning Confocal Microscope Zeiss 710 Laser Scanning Confocal Microscope Zeiss 510 META Laser Scanning Confocal Microscope Fluorescence microscopy Zeiss Imager M2 + Apotome Zeiss Observer Z1 Zeiss PALM Laser Microdissection system Calcium imaging Improvision system for time-lapse imaging Micro CT SkyScan-1072 High-resolution desk-top micro-CT system Live cell Imaging Delta Vision Core wide field imaging system with EMCCD Histology Tissue processors, standard and motorized microtomes for wax and resin sectioning Cryostat for frozen sectioning Leica Bond-max fully automated immuno-stainer Electron Microscopy 2x Philips CM10 TEMs with Gatan CCD digital imaging Zeiss EVO MA10 SEM with Oxford Inca microanalysis Leica UC6, Ultracut S & Ultracut E ultramicrotomes Cryo Electron Microscopy Leica Empact 2 High Pressure Freezer (with rapid transfer system) Leica AFS (Automated Freeze Substitution) processor Leica FC6 cryo-ultramicrotome
Belfast Stewart Church +44 (0)2890972274 Bioimaging Core Technology Unit School of Medicine Dentistry and Biomedical Sciences Queens University of Belfast Medical Biology Centre 97 Lisburn Road Belfast BT9 7AB Northern Ireland Access Internal on all instruments External commercial & academic on application DIY or full support service Proof of principle/ developmental studies undertaken Expertise Core Facility, dedicated staff for each imaging platform. Computational methods for quantitative image analysis. Content Management Systems for all images, central server with remote access. Workshops, training courses, teaching. Embedded Cat 2 facilities with microbiological safety cabinets and animal holding suite.	Confocal Microscopy Leica TCS SP2 with DMRBE/DMIRE2 Leica TCS SP5 with DMI6000+Solent live cell chamber (Cat 2 facility) Fluorescence Microscopy Zeiss Axiovert 200 with Axiovision Leica DMI6000B with FW4000 Live Cell Imaging Zeiss Axiovert 200 with Eppendorf Injectman +Femtojet (Cat 2 facility) Virtual Microscopy Aperio CS Scanner Aperio OS Scanner Hamamatsu Nanozoomer in vivo Imaging Xenogen IVIS 200 FACS BD FACSAria II (Cat 2 facility)
Birmingham, BALM Manuel Forero (Imaging Specialist) +44 (0)121 414 3392 Alicia Hidalgo (co-director of BALM) +44 (0)121 414 5416 Birmingham Advanced Light Microscopy Facility (BALM) School of Biosciences University of Birmingham Edgbaston Birmingham B15 2TT Access Internal, mostly used by School of Biosciences External academic: used by many other Schools within this University External commercial subject to access charges Can be used as DIY or with technical help Expertise Core facility, confocal microscopy. Fixed, time-lapse and cell culture. Computational methods for quantitative image analysis, object recognition and cell tracking. Future requirements Computational methods for imaging	Confocal microscopy Leica SP2 AOBS inverted (4 lasers) BioRad Radiance 2000 upright (3 lasers)
Birmingham, BALM-AWN Joshua Rappoport (co-director of BALM) +44 (0)121 414 9019 Birmingham Advanced Light Microscopy - Advantage West Midlands Imaging Facility (BALM-AWM) School of Biosciences University of Birmingham Edgbaston Birmingham B15 2TT Access Internal (university-wide) External Expertise Core facility - Live cell imaging; Probe development and high resolution imaging	Confocal microscopy Nikon A1R confocal/TIRF with spectral detector, EM-CCD, and Piezo-Z drive
Bristol Mark Jepson (light microscopy) +44 (0)117 33 12143 Paul Verkade (electron microscopy) +44 (0)117 33 12179 Prof. Paul Martin +44 (0)117 3312298 Wolfson Bioimaging Facility School of Medical Sciences University of Bristol University Walk Bristol BS8 1TD Access Currently most use is Bristol-based and subject to access charges Scope for external collaborations in speciality areas We work on a recharge basis both for full service (EM) as well as DIY. Expertise Core facility. CLEM - Correlative light and electron microscopy. Fully integrated Light and Electron Microscopy Unit. Live cell imaging (especially intracellular trafficking, model organisms, bacterial infection). Future requirements Tools for image analysis, especially tracking.	Confocal microscopy 4 Leica CLSM systems: inverted SP5 AOBS, SP2 AOBS, SP2; upright NT Spinning disk confocal: Perkin Elmer UltraVIEW ERS 6FE Wide-field microscopy 3 Leica AS-MDW 2 Leica microscopes with Hamamatsu cameras, automated filter wheels and shutters, Improvision Volocity software TIRF (Leica AM TIRF MC system for total internal reflection fluorescence microscopy or wide-field imaging) Electron microscopy FEI Tecnai 12: 120kV transmission electron microscope FEI Tecnai 20: 200 kV transmission electron microscope equipped with a STEM unit; suitable for cryo-EM and electron tomography, including STEM tomography. FEI Quanta 400 variable pressure scanning electron microscope Extensive EM preparation facilities. Other Offline image analysis suite for LM and EM. Almost all Light Microscopes are equipped with live cell imaging chambers. Cell culture facilities within the facility.
Cambridge Richard Adams +44 (0)1223 333782 Department of Physiology, Development and Neuroscience University of Cambridge Downing Street Cambridge CB2 3DY Expertise Time-lapse imaging, image processing, computational methods	Image analysis. Cell tracking and cell shape analysis in whole embryos. Computational analysis of tissue dynamics during embryogenesis.
Cambridge Matt Wayland +44 (0)1223 769282 Imaging Facility Department of Zoology University of Cambridge Downing Street Cambridge CB2 3EJ Access Internal (80% on all instruments) External commercial & external academic (20% on all) DIY or full service work Expertise Core Facility Image analysis and programming	Confocal microscopy Leica SP5 invert Leica SP5 upright Leica SP upright UltraView CSU-10 Spinning Disk MicroPoint laser ablation / microdissection Live cell imaging chamber on SP5 invert (up to 37^oC and 5% CO2
Cambridge Alexandre Kabla +44 (0)1223 766208 Department of Engineering University of Cambridge Trumpington Street Cambridge CB2 1PZ Expertise Imaging, computational methods	Image Analysis Image processing and mechanical properties of multicellular assemblies
Cambridge Clemens Kaminski +44 (0)1223 763135 Laser Analytics Group Department of Chemical Engineering and Biotechnology University of Cambridge Pembroke Street Cambridge CB2 3RA Access Internal External DIY Expertise All aspects of quantitative multidimensional microscopy	Confocal microscopy Custom built widefield FLIM platform with LED and Supercontinuum excitation (430-700 nm). Both ultrahigh speed FLIM (15 fps at full field) and excitation at multiple modulation frequencies are possible. Custom designed hyperspectral imaging confocal station, with supercontinuum excitation (430-700 nm excitation / emission, at electronically selectable, arbitrary wavelength combinations). Full resolution of fluoresence excitation and emission spectrum, fluorescence lifetime and polarisation state. TCSPC, Fluorescence Anisotropy Imaging Leica SP5 confocal platform with resonant scanner.
Cambridge David Klenerman +44 (0)1223 336481 The University Chemical Laboratory Dept of Chemistry University of Cambridge Lensfield Road Cambridge CB2 1EW Expertise Laser fluorescence spectroscopy and scanning probe microscopy	Fluorescence microscopy Two colour total internal reflection single molecule fluorescence microscope
Cambridge Jeremy Skepper +44 (0)1223 333774 Janet Powell +44 (0)1223 333774 Multi-Imaging Centre Anatomy Building Physiology Development and Neuroscience, University of Cambridge Downing Site Cambridge CB2 3DY Access Internal External Commercial. DIY and service work. Expertise Specimen preparation, ambient and low temperature, stereology, x-ray microanalysis, immunocytochemistry, bio-materials microscopy, physiological and time-lapse microscopy.	Confocal microscopy and LMD Leica SP1- multiphoton (invert) Leica SP1-UV (invert) Leica SP2-405 - 633 (invert) Leica SP2-456 - 633 (invert) Leica SP5-456 - 633 (invert physiology) Leica LMD 6000 (upright laser microdissection microscope) Electron microscopy FEI Technai G2 200 kv, STEM, tomgraphy, cryo-transfer, HAADF, SAED, CBED, EDAX Genesis EDX, Deben AMT digital camera FEI-Philips XL30 FEGSEM, SE and BSE detectors Oxford INCA EDX with 30mm pentafet ATW detector Quorum Emitech PP2000 cryotransfer system Specimen preparation Leica VT1000S vibrating blade microtome Leica CM 3050S Cryostat Leica CPC plunge and impact freezing device 2 x Leica AFS freeze substitution and low temperature embedding devices 3 x Leica Ultracut E Leica Ultracut UCT Leica Ultracut S and FCS cryosectioning device Edwards 306 auto, Carbon coater, freeze drier and glow discharge unit Quorum Emitech K575X dual head Au and Cr sputter coater and remote pumping station for TEM cryo-transfer specimen holder Polaron CPD
Cambridge, Babraham Simon Walker +44(0)1223 496618 Imaging Facility The Babraham Institute Babraham Cambridge CB22 3AT Access Full access to external users, though internal users get priority. DIY or Full service work Expertise Core Facility Live cell fluorescence imaging	Confocal microscopy Zeiss LSM 510 META invert Olympus FV1000 invert (x2) Spinning Disk with EM CCD (Andor) Fluorescence imaging Olympus CellR (widefield+TIRF) Live cell imaging chamber High Content Screening BD Pathway 855 MetaSystems Metafer / MetaCyte
Cambridge, Centre for Stem Cell Research Peter Humphreys (light microscopy) +44(0)1223 760200 Rachael Walker (flow cytometry) +44(0)1223 760227 Centre For Stem Cell Research Tennis court Road Cambridge CB2 1QR Expertise Core Facility, image analysis Future requirements HE/fluorescent upright Plate scanning microscope	Confocal Microscopy Leica SP5 invert + live cell chamber x2 Andor Revolution XD spinning disk Widefield and cell culture Nikon Biostation IM Essen Incucyte HD Essen Incucyte Zeiss AxioExaminer Leica DMI4000 Olympus IX51 x3 Flow Cytometry MoFlo CyAN
Cambridge, Gurdon Inst. Alex Sossick +44 (0)1223 334082 Wellcome Trust/Cancer Research UK Gurdon Institute University of Cambridge Tennis Court Road Cambridge CB2 1QN Access Priority given to Gurdon Institute users Limited outside access Expertise Core Facility. Immunofluorescence; widefield epifluorescence and confocal microscopy, deconvolution	Confocal microscopy Confocal scanning laser microscopes and multi-photon system Widefield deconvolution and high throughput systems
Cardiff Jim Ralphs +44 (0)2920 874954 Tony Hayes +44 (0)2920 876611 Marc Isaacs +44 (0)2920 876611 Confocal Microscopy Unit Cardiff School of Biosciences Cardiff University Cardiff CF10 3AX Access Internal (mainly), but also external commercial & academic Expertise Core Facility; Immunofluorescence; Widefield epifluorescence and confocal microscopy	Confocal microscopy Leica TCS SP2 AOBS laser scanning confocal microscope. Twin microscope configuration comprising upright (DM6000) and inverted (DMIRE2) stands (laser lines: 405, 458, 476, 488, 514, 543 & 633nm), galvonometer & electromotor stages; Solent Scientific incubation chamber with CO2/humidity and temperature regulators for in vitro work; brightfield, fluorescence, DIC and polarizing optics Fluorescence imaging Olympus BX series microscopes with fluorescence, DIC and brightfield optics refer to web pages for further information
Cardiff Anna Hurley +44 (0)2920 687004 Charlotte James +44 (0)2920 687004 Central Biotechnology Services Henry Wellcome Building School Of Medicine Cardiff University Heath Park Cardiff CF14 4XN Access Internal and external users Academica and Industry User access or managed service Expertise Core facility, academic consultancy, certified to ISO 9001 and ISO 17025	Confocal microscopy Leica SP5 Leica SP2 Flow cytometry 3 x FACS Calibur 4 colour 1 x Beckman Cyan ADP 9 colour 1 x FACS Canto II 8 colour 1 x FACS Calibur 3 colour Beckman MoFlo - 9 parameters simultaneously using 6 colours
Cardiff Jan Hobot +44 (0) 2920 742131 Chris von Ruhland +44 (0) 2920 742132 Medical Microscopy Sciences School of Medicine Cardiff University Heath Park Cardiff CF14 4XN Access Internal and external both academic and commercial. Expertise Core facility. Specimen preparation at ambient or low temperature for structural or immunocytochemical studies. First-hand knowledge of modern acrylics (Lowicryls and LR White). Tokuyasu or cryosubstitution methodologies. Histology and LM preparations. TEM and SEM X-ray microanalysis. UKAS accreditation for X-ray microanalysis of materials and particulates for detecting asbestos.	Fluorescence imaging Olympus BX51 Upright with Zeiss AxioCam camera. Olympus CK40 Inverted with Polaroid digital camera. Reichert Polarising Microscope. Electron microscopy Philips CM12 TEM with SIS MegaView III camera and EDAX Genesis X-ray microananalysis system. JEOL 840A SEM with ADDA SIS digital imaging and INCA x-sight detector X-ray microanalysis system with elemental mapping plus Oxford CT1500 Cryosystem and stage. Specimen Preparation Leica EMTP Tissue Processor. Reichert KF80 metal mirror/plunge freezer. Dedicated Histology room.
Didcot, STFC David Clarke +44 (0)1235 567035 Octopus Imaging Cluster (Lasers for Science Facility) Science & Technology Facilities Council Rutherford Appleton Laboratory Didcot OX11 0QX Access Via peer-review panel for external and internal users Approx 80% of scheduled time for long-term (>1 year) programmes, and 20% for proof-of-principle work Additional access for users of other facilities (Diamond Light Source, ISIS, Research Complex at Harwell) Commercial access also available Expertise Core Facility; Single molecule techniques, including in live cells; single molecule and ensemble FRET; FLIM; development of advanced laser microscopy instrumentation. Future requirements Image analysis for single molecule work	Central laser cluster (ns, ps, fs and steady state lasers UV-near IR) feeding the following microscopy stations: Single molecule microscopy Multidimensional (simultaneous two-colour(FRET)/two polarisations) single molecule TIRF microscope (live cell and immobilised samples). 3-colour single molecule TIRF X-Y-t tracking (Multiprotein complex kinetics and dynamics). 5-colour single molecule TIRF X-Y-t tracking (Multiprotein complex kinetics and dynamics) - under construction - to be scheduled next year. Confocal microscopy Multiphoton confocal fluorescence microscope for FRET/FLIM Imaging. Multicolour confocal fluorescence microscope with Becker & Hickl Fluorescence Lifetime Imaging (FLIM) module. Support Equipment Laser tweezers, steady-state and time-resolved laser spectroscopy apparatus, cell biology and biochemistry support labs
Dundee Jason Swedlow +44 (0)1382 385819 Swedlow lab Wellcome Trust Centre for Gene Regulation & Expression College of Life Sciences MSI/WTB/JBC Complex University of Dundee Dow Street Dundee DD1 5EH Open Microscopy Environment Expertise Structured illumination microscopy on OMX, expertise on high content screening and live cell imaging, development of data management tools and specifications for microscopy. Future requirements Money for meetings and travel to other sites for access and sharing of info and smart, programmable microscopes.
Dundee Sam Swift +44 (0)138 238 6412 Central Microscopy Facility College of Life Sciences MSI/WTB Complex University of Dundee Dow Street Dundee DD1 5EP Access User training/DIY for light microscopy DIY or Full Service for EM External academic / industry welcome by appointment Expertise Core facility; High resolution imaging, live cell imaging, FLIM, FRET, FRAP, immuno EM etc	Light Microscopy BioRad Radicance 2100MP confocal / multiphoton Zeiss LSM 510 META Leica SP2 Becker and Hickl TCSPC FLIM DeltaVision Personal, RT and Core restoration microscopes with EMCCD, Live Cell Chambers and Laser Modules Nikon TIRF OMX Structured Illumination Microscope Electron Microscopy FEI Tecnai 12 TEM Jeol 1200 EX TEM Hitachi s4700 FESEM
Dundee Markus Posch (SULSA Scientific Officer for OMX) +44 (0)138 238 6414 OMX Super-Resolution Microscopy College of Life Sciences University of Dundee Dow Street Dundee DD1 5EH Access Any users welcome Euro BioImaging Proof of Concept site(Advanced Light Microscopy - General Access) Expertise Structured Illumination Spatial Super-Resolution - Superfast Live Imaging	OMX ver. 3 platform (Applied Precision)
Durham Tim Hawkins (Advance Light Microscopy) +44 (0)191 334 1333 Christine Richardson (Electron Microscopy) +44 (0)191 334 1285 Microscopy and Bioimaging Facility School of Biological & Biomedical Sciences Durham University Durham DH1 3LE Access Internal on all instruments External commercial & external academic DIY or Full Service Expertise Core facility; High resolution imaging, FRET, FRAP, FLIP. 5D live cell imaging, TIRF, photoactivation/conversion, ImmunoGold, Cryosectioning, Ultramicrotomy, Sterology. Licenced to image plant:pathogen interactions within facility. Collaboration & developments of new techniques (eg photonics, chemical probes) Future requirements SuperResolution, FCCS & FLIM	Confocal microscopy Leica TCS SP5 Tandem head AOBS Invert (Resonant scanner) Zeiss LSM 510 META invert BioRad Microradiance Upright Andor Revolution XD Spinning Disk Invert Fluorescence Imaging Leica MC TIRF Microscope Zeiss Apotome Microscope Zeiss Live Cell Imaging System EM CCD Imaging Most of the above microscopes are within live cell imaging/environmental chambers Microinjection Upright (DM2500) and inverted (DMI3000) DIC microscopes + multiple injection systems to work with a wide variety of organisms and tissues. HD imaging system. Electron Microscopy Hitachi H7600 TEM High resolution Hitachi S5200 FESEM + Cryostage Image analysis Suite of high-end 64-bit PC workstations Deconvolution Sample Preparation Leica High Pressure Freezer EMPACT Leica AFS freeze substitution and low temperature Leica Ultracut S room temperature Leica FC6 cryo-ultramicrotome Leica Knife maker Baltec Critical Point Drier Cressington coating units (Carbon, platinum, chromium, tungsten) Leica CPC cryo workstation Leica IGL. Automatic immunogold labeller Leica MM80E metal mirror/plunge freezer Tissue processing Cryostat for frozen sectioning Microtomes for wax and resin sectioning Leica vibrating diamond knife
Edinburgh Rolly Wiegand (LM) +44 (0)131 650 2864 CALM - Confocal and Advanced Light Microscopy Facility Shonna Johnston (Flow) +44 (0)131 242 6567 Flow Cytometry Facility Queen's Medical Research Institute 47 Little France Crescent Edinburgh EH16 4TJ Access LM Internal for University External DIY or fully assisted image acquisition Flow Internal for University only Fully assisted cell sorting Expertise Core Facility; Teaching - Microscopy course (twice a year), high resolution cellular microscopy, image restoration and quantitation, live cell imaging Future requirements Hardware for deep penetration in/ex vivo live cell imaging and TIRFM	Confocal microscopy Zeiss LSM 510 META, inverted Leica SP5, inverted, both eqipped with environmental control Fluorescence imaging Olympus Cell P, inverted Environmental control for live cell imaging Optical Projection Tomography (Bioptonics 3001) Image analysis High-end workstations with SVI Huygens Essential, ImageJ and PE Volocity Flow cytometry Cytometry analysers BD FACSCalibur BD FACScan BD FACSArray Cell sorters BD FACSVantage SE (Diva) BD LSR Fortessa BD FACSAria II
Edinburgh, Clinical Research Imaging Centre (CRIC) Edwin J. R. van Beek +44 (0)131 242 7760 SYNAPSE Chair of Clinical Radiology Stuart Docherty +44 (0)131 242 7767 Clinical Research Imaging Centre University of Edinburgh CO.19 Queen's Medical Research Institute 47 Little France Crescent Edinburgh EH16 4TJ Access Access through applications process PET-CT 70% NHS service MDCT 50% NHS service MRI 100% research Expertise Core facility. Imaging-based assessment of structure, function and treatment efficacy	Imaging 3T MRI (Siemens Verio) with fMRI, multinuclear spectroscopy and MR Elastography Mock MRI system 320 MDCT (Toshiba Aquilion ONE) 128 mCT-PET (Siemens) PET-trace 8 cyclotron (GE) Radiochemistry 4 GMP hot cells and 3 research hot cells Image analysis laboratory
Edinburgh, Orthopaedic Engineering Collaboration Antonello Spadaccino +44 (0)131 242 6295 Orthopaedic Engineering Collaboration Chancellor's Building University of Edinburgh Room FU 413 49 Little France Crescent Edinburgh EH16 4SB Access Internal (75%) External and commercial (25%) Full service work Induction training and supported DIY Expertise Scan and production of 3D images from samples. Comprehensive tensile, 3 & 4 point bend tests. Torsional testing, ultimate strength, fatigue and step loading. Combination of axial and torsional tests.	Xray Imaging Skyscan 1172 Mechanical Testing Zwick/Roel z0005
Edinburgh, IMPACT Trudi Gillespie +44 (0)131 6503278 IMPACT Imaging Facility Hugh Robson Building George Square Edinburgh EH8 9XD Access Internal for University External DIY or fully assisted image acquisition Expertise Core Facility. High resolution cellular microscopy, image restoration and quantitation, live cell imaging, TRIF and FLIM Future requirements MP system for in/ex vivo live cell imaging	Confocal microscopy 2 Zeiss LSM 510 one inverted and one upright both coupled to a Coherent Mira mode-locked Ti:Sapphire multi-photon laser equipped with environmental control and a Becker and Hickl unit for fluorescence lifetime imaging (FLIM). Leica TCS-NT, inverted and upright with environmental control Fluorescence imaging Olympus Cell R Dual TIRF System, with environmental control for live cell imaging Image analysis High-end workstations with SVI Huygens Essential and Pro, ImageJ, Imaris and PE Volocity
Edinburgh, MRC Mike Millar +44 (0)131 242 9155 Histology and Imaging Facility MRC, Human Reproductive Sciences Unit The Queens Medical Research Institute New RIE 47 Little France Cres Edinburgh EH16 4TJ Access Internal on all instruments External academic on application DIY or Full service work Expertise Core facility. Confocal microscopy, Stereology and immunohistology with emphasis on multi same species colocalisations. Reproductive morphology	Confocal microscopy Zeiss 510 Laser Scanning Confocal Microscope Zeiss 510 META Laser Scanning Confocal Microscope Zeiss 710 Laser Scanning Confocal Microscope Widefield microscopy Assorted upright and invert research grade microscopes with CCD imaging both in colour and monochrome Live cell Imaging Stereology using Media Cybernetics Image Pro Plus with stereologer plug in Image Analysis Huygens deconvolution software Volocity 3D imaging software Media Cybernetics Image Pro Plus Histology Tissue processors, standard and motorized microtomes for wax and resin sectioning Cryostat for frozen sectioning Leica Bond- fully automated immuno-stainer Intavis whole mount in-situ hybridisation and immunostaining robot
Edinburgh, Brain Research Imaging Centre (BRIC) Joanna Wardlaw (Director of Centre) +44 (0)131 537 2943 (PA) Duncan Martin (Scientific Business Manager) +44 (0)131 537 2664 Brain Research Imaging Centre University of Edinburgh Division of Clinical Neurosciences Bramwell Dott Building Western General Hospital Edinburgh EH4 2XU Access Access through an application process Research and commercial scanning work can be undertaken Expertise Stroke, FMRI, Diffusion and tractography, Perfusion imaging, Aging, Spectroscopy, Psychiatric disorders, Neurodegenerative disorders	1.5T (GE) MRI imaging capabilities Structural Diffusion and Tractography Functional MRI (Simultaneous EEG) Perfusion and Bloodflow Permeability Spectroscopy Temperature Mapping Radiological advice on imaging and study protocols Radiological interpretation of scans Image Analysis methods MRI Quality Assurance methods Software tools for image analysis and image management
Essex Philippe Laissue +44 (0)1206 872246 Bioimaging Facility Department of Biological Sciences Wivenhoe Park University of Essex Colchester CO4 3SQ Access Internal on all instruments External commercial & academic on application; Nikon reference site Proof of principle studies undertaken Expertise Core facility, rapid and long term live cell imaging, immunofluorescence, colocalisation, visualisation. Development of specialised instruments and computational methods for quantitative image analysis. Future requirements Further in-house development of instruments and algorithms. Super-resolution and FLIM.	Confocal microscopy Nikon A1R (inverted) with 6 laser lines, hybrid scanner, spectral detector, automated XY and piezo Z, TIRF/OAF/widefield with EM-CCD, live cell chamber Bio-Rad Radiance 2000 with Olympus IX70 (inverted) Widefield fluorescence Microscopy Nikon Ti-E (inverted) with LEDs and Intensilight, EM-CCD, automated XY and piezo Z, live cell chamber In-house developed TIRF and OAF instrument (inverted) with EM-CCD and triple split (Kad group) Various upright and inverted fluorescence and brightfield microscopes with EM-CCD and colour CCD cameras Several commercial and in-house developed imaging systems for measurement of photosynthesis by gas exchange and fluorescence, both in the laboratory and in the field (Lawson group). Image Analysis High end 64-bit workstations for offline image processing and analysis. NIS-Elements, deconvolution. Custom software using ImageJ and Matlab. Workshops, training courses, teaching. Extensive website for booking, training, resources. Other Fully equiped Cat 2 laboratory, cell culture facilities Flow Cytometry BD FACS Aria BD FACS Calibur
Glasgow, Beatson Kurt Anderson +44 (0)141 330 2864 Beatson Institute for Cancer Research Garscube Estate Switchback Road Glasgow G61 1BD Access Priority given to Beatson users, followed by Glasgow University Outside access primarily on a collaborative basis Expertise Live cell imaging, including time and frequency domain FLIM, TIRF, photo-activation / -bleaching / -conversion. Mouse in vivo imaging, including collagen SHG and use of photo-activation and -bleaching, FLIM and FRET. Future requirements Image analysis, adaptive optics	Confocal microscopy Olympus FV1000 (x2) Nikon A1R Leica TCS SP2 Zeiss LSM 710 FLIM LaVision Bio-Tec TRIM scope Lambert Instruments custom Spinning Disk Lambert Instruments custom TIRF Leica TCS SP2 with Becker & Hickle TCSPS 730 Live Cell Custom TIRF Olympus / Volocity Deconvolution system Micro-injection Medium Throughput Long Term Time Lapse (x4) Other Olympus OV100 Leica AF6000 micro-dissection FacsAria Upright stands for histology and immunofluorescence (x4) Off-line image analysis (x8)
Leeds Gareth Howell +44 (0)113 3437270 Leeds Bioimaging and Flow Cytometry Facility Faculty of Biological Sciences University of Leeds Leeds LS2 9JT Access Internal and external, academic research groups and commercial Expertise Core Facility. Collaboration and development of techniques, image processing and analysis. Live cell imaging.	Confocal microscopy Zeiss LSM 510 META invert with motorised stage and 405 laser diode Zeiss LSM 510 META upright with motorised stage Fluorescence imaging Delta Vision Deconvolution System Live cell imaging chambers Microinjection Eppendorff FemtoJet system on an inverted Zeiss Axiovert 200 Flow cytometry FACSCalibur 4 colour analyser flow cytometer FACSAria 7 colour cell sorter Partec PASIII 4 colour flow cytometer
Leicester Kees Straatman + 44 (0)116 229 7085 Advanced Imaging Facilities Department of Biochemistry Henry Wellcome Building University of Leicester Lancaster Road Leicester LE1 9HN Access Mostly internal users but external users are welcome Expertise Core Facility. Immunofluorescence, widefield epifluorescence, confocal microscopy, live cell imaging, microinjection, FRAP/FLIP/FRET, spectral imaging, high content screening/analysis. Data analysis and quantification: Imaris, Volocity, Openlab, Huygens Essential deconvolution, ScanR/CellR, ImageJ, NIS Elements. Teaching: Microscopy workshops, lectures Future requirements Technical support Image indexing software FCS/FLIM Super resolution imaging Whole animal imaging (funding secured)	Most systems with incubators, temp and CO₂ control Confocal microscopy Leica TCS SP5 inverted Olympus FV1000 inverted Nikon C1Si inverted Zeiss LSM 7 MP upright (soon) Fluorescence imaging 3 Nikon TE 300 inverted 1 Nikon Ti-E EM-CCD + LEDs inverted Olympus CellR/scanR inverted 1 Nikon TIRF EM-CCD inverted Olympus BX61 upright with Applied Spectral Imaging's scanning platform Bioluminescence imaging Olympus LV200 EM-CCD Flow cytometry BD FACS Canto II Imagers Typhoon Trio+ LAS4000
London, Imperial Raffaella Carzaniga +44 (0)207 594 5194 Electron Microscopy Centre Imperial College Exhibition Road South Kensington Biochemistry Building- Room 022 London SW7 2AZ Access Internal and external academic research groups and commercial Expertise Cryo-Preparation methods for TEM, TEM for Cellular Biology & Single Particles. Image processing for Single Particles	Electron microscopy TEM-Philips CM100 (Tungsten electron source) with film plate TEM- FEI Tecnai 12 (LaB6 electron source); CCD camera TEM Philips CM200 (FEG electron source); CCD camera TEM- Philips CM300 (FEG electron source); Helium cooled-Polara Stage; CCD camera Vitrobot HPF- High Pressure Freezing apparatus (EMPACT 2 with RT, Leica) Ultramicrotomes (for ambient and low temperatures) Parallel computer farm
London, Imperial Dirk Dormann +44 (0)208 383 8528 Microscopy Facility MRC Clinical Sciences Centre Faculty of Medicine Imperial College London Hammersmith Hospital Campus Du Cane Road London W12 0NN Access Core funded by MRC, only MRC users Expertise Core facility. Quantitative image analysis, time-lapse	Confocal Microscopy Leica SP1 invert Leica SP2 invert Leica SP5 invert with tandem scanner and FCS/FCCS PerkinElmer UltraView Spinning Disk Fluorescence imaging Deltavision Spectris Leica DMIRE2 inverted with Eppendorf microinjection system Leica DMRB upright Zeiss Axiovert 100S TV inverted Zeiss Axiophot upright Olympus SMZ-16X stereo microscope PALM laser capture/microdissection
London, Imperial Paul French +44 (0)207 594 7713 Photonics Group Physics Department Imperial College London South Kensington Campus London SW7 2AZ Access By collaboration Expertise Optical Microscopy, Optical Tomography, Optical Endoscopy, Imaging Data Management and Integration, Optical Image Analysis Future requirements Multidisciplinary training at the interface of photonics technology and life science applications. Long term funding for core instrumentation staff. Tb scale optical image data management systems. Software tools including automatic image segmentation and multidimensional analysis.	Multidimensional fluorometer Confocal/Multiphoton Microscope, Ti-Sapphire laser or supercontinuum excitation source/FLIM/polarisation-resolved imaging Line-scanning semiconfocal microscope with Ti-Sapphire laser or supercontinuum excitation source TIRF microscope, multispectral imaging, FLIM/polarisation resolved imaging capability Wide-field FLIM microscope optimised for UV excitation Multibeam multiphoton microscope (LaVision TriMScope) with FLIM High throughput automated optically sectioned (Nipkow) FLIM microscope High speed Nipkow disc FLIM microscope with supercontinuum excitation STED FLIM microscope, Leica SP2 laser scanning confocal, Ti:sapphire laser Wide-field FLIM endoscope/macroscope system Microconfocal FLIM endoscope FLIM Optical Projection Tomography (OPT)
London, Imperial Martin Spitaler +44(0)207 594 2023 FILM (Facility for Imaging by Light Microscopy) Imperial College Exhibition Road South Kensington / SAFB London SW7 2AZ Access Internal and external (academic and commercial) DIY or assisted microscopy Assay development Expertise Core facility	Confocal microscopy Zeiss LSM-510 Leica SP2 Leica SP5 resonant Leica SP5 MP-Flim upright and inverted Widefield microscopy EM-CCD Incubation chambers Automated XYZT imaging Low and medium throughput TIRF Image analysis Volocity SimplePCI Autoquant CellProfiler Matlab Definiens
London, King's Rainer Heintzmann Matthias Gautel Randall Division of Cell and Molecular Biophysics King's College London New Hunt's House Guy's Campus London SE1 1UL Access Internal Collaborations to apply superresolution imaging are possible DIY Expertise Superresolution Microscopy. Facilities for structured illumination, 4pi, STED (soon), expertise in deep tissue imaging, FRET and FLIM. Future requirements Financial support for personnel for applying existing expertise to critical biological problems	Structured Illumination Lecia 4Pi TCS SP2 Leica STED
London, King's Christopher Healy +44 (0)207 188 1795 Tissue Imaging Centre, Dept of Craniofacial Development, King's College London Dental Institute at Guy's, King's College and St Thomas' Hospitals, Floor 27 Tower Wing, Guy's Hospital, St. Thomas St., LONDON SE1 9RT Access Internal (100% on microscopes, 90% on microCT) External commercial & external academic (10% on all) DIY on microscopes. Part or Full service work on MicroCT and OPT Expertise Core facility. Morphometrics, bone histomorphometry and high resolution imaging.	Confocal microscopy Leica SP5 upright confocal microscope Fluorescence imaging Zeiss Axioskops with Axiocamera PALM laser capture/microdissection Live cell imaging chambers microCT GE Healthcare Locus SP specimen microCT Optical Projection Tomography Bioptonics 3001
London, CRUK-LRI Daniel Zicha (LM) +44 (0)207 269 3563 Lucy Collinson (EM) +44 (0)207 269 3416 Anne Vaahtokari (Super-resolution microscopy) +44 (0)207 269 3511 Cancer Research UK, London Research Institute Lincoln's Inn Fields Laboratories 44 Lincoln's Inn Fields London WC2A 3PX Access LM - Priority given to LRI users, limited outside access EM - Core Technology Facility, internal groups only Super resolution - Priority given to LRI users, limited outside access Expertise Core facility. Expertise in image processing and simulation. Quantitative analysis with multi-modal microscopy. EM - Preparation of cell biological material for TEM and SEM. Cryo preparation techniques for biological samples (HPF, FS, Tokuyasu, freeze fracture, freeze drying, cryoEM) Tomography Correlative Light/ Electron Microscopy (CLEM) Volume imaging (FIB/SEM, SBF/SEM, MicroCT, NanoCT) Super-resolution microscopy Future requirements Support for developing new image processing approaches	Confocal microscopy LSM 510 (Zeiss) including a multiphoton system Leica SP5 Low light level imaging systems Universal Imaging Metamorph, Kinetic Imaging AQM Molecular Devices Discovery-1 PerkinElmer UltraVIEW confocal imaging systems Eppendorf Microinjection systems Electron Microscopy Unit Electron microscopy FEI Tecnai G2 Sprit TEM 120kV, with tomography stage, single and dual tilt tomography holders, bottom mount Gatan Ultrascan CCD, side mount Gatan Orius CCD FEI Tecnai G2 Sprit TEM 120kV, with cryo stage and cryo holder, bottom mount Gatan Ultrascan CCD, side mount Gatan Orius CCD Jeol 1010 TEM with side mount Gatan 1kx1k CCD Jeol JSM 6700F Field Emission SEM Specimen preparation Leica High Pressure Freezer EMPACT2 and RTS 2 Leica AFS freeze substitution and low temperature embedding devices (1 AFS1, 1 AFS2 with FSP) 2 Leica Ultracut UCT room temperature Reichert Ultracut S room temperature 2 Leica Ultracut UCT, FCS cryo Knife maker Baltec Med020 dual gun sputter coater (carbon and carbon/platinum) and rotary shadower 2 Critical Point Drier 3000 Polaron sputter coater Gatan ion beam coater Emitech freeze substitution K775X FEI Vitrobot Mark IV Software expertise Gatan Digital Micrograph Inspect 3D Amira Imaris Drishti Super-resolution Microscopy API OMX V3 structured illumination super-resolution microscope
London, CRUK-LRI Francois Lassailly +44 (0)207 269 3426 In vivo Imaging Facility London Research Institute Cancer Research UK 44 Lincoln's Inn Fields London WC2A 3PX Access Primarily for LRI users but could be discussed for external users Expertise Non-invasive in vivo imaging. 2D, 3D, 4D imaging. Longitudinal studies for orthotopic models in oncology and hematology, normal development, stem cells, microenvironment, vascular and bone biology. Assessment of validation of probes and contrasting procedures. Image processing and analysis	Whole body Bioluminescence (including diffuse optical tomography and spectral unmixing) Caliper Life Sciences IVIS-Spectrum Whole body Fluorescence (including epifluorescence, transillumination, tomography and spectral unmixing from UV to IR)) Caliper Life Sciences IVIS-Spectrum Micro-CT (including physiological monitoring for respiratory or cardiac gating, 4D) Skyscan 1176 in vivo scanner Intravital microscopy of live tissues (combining multiphoton and confocal, equiped with multiparametric physiological monitoring) Zeiss LSM710-NLO
London, LSHTM Theresa Ward +44 (0)207 612 7869 Liz McCarthy and Rachel Gregory (LM) +44 (0)207 927 2063 Maria McCrossan (EM) +44 (0)207 927 2373 Liz King (Flow Cytometry) +44 (0)207 927 2373 Woolfson Cell Biology Facility Immunology Unit London School of Hygiene & Tropical Medicine Keppel Street London WC1E 7HT Access Internal (90% on all instruments) External academic (10%) DIY or Full service work Expertise Imaging pathogen-host interactions, at cellular level, and in whole model organisms. Includes live imaging under Cat 2 and full aerosol Cat3 containment. Future requirements Multiphoton for cell and in vivo imaging under containment	Confocal microscopy Zeiss LSM 510 invert with live cell chamber and Eppendorf microinjeciton Fluorescence imaging Zeiss Axioplan 2 upright with CCD, epifluorescence and histology Keyence BZ-8000 automated fluorescence microscope under Cat3 containment Volocity software Laser capture/microdissection Arcturus Pixcell IIe In vivo imaging under Cat3 containment IVIS Spectrum (Xenogen) Fluorescence molecular tomography (FMT) system Electron microscopy Jeol JEM-1200 EXII TEM Jeol JSM-25S III SEM Flow cytometry 2x Becton Dickinson FACSCalibur (one under Cat3 containment) Becton Dickinson FACSVantage SE cell sorter
London, National Physical Laboratory Alex Knight (Biotechnolgy Group) +44 (0)208 9436308 Daniel Metcalf (Biotechnology Group) +44 (0)208 9436344 National Physical Laboratory Analytical Sciences Division Hampton Road Teddington TW11 0LW Access Internal External commercial contract research External academic collaborations welcome Expertise Super-resolution microscopy, Single molecule fluorescence, Confocal microscopy, Adaptive optics, Optical coherence tomography, Instrumentation, Metrology/standards	Super-resolution Microscopy: Direct stochastic optical reconstruction microscope (self-build) Single molecule fluorescence microscope (self-build) Structured illumination microscope (self-build, ready early 2012) Photoactivated localisation microscope (self-build, ready early 2012) Other imaging techniques available at NPL include: Confocal fluorescence microscope (Olympus FV1000), Scanning ion conductance microscopy, atomic force microscopy, raman microscopy, secondary ion mass spectrometry, optical coherence tomography, X-ray photoelectron spectroscopy, matrix assisted laser desorption ionisation mass spectrometry, scanning electron microscopy, terahertz imaging, ultrasound
London, NIMR Yan Gu +44 (0)208 816 2621 Confocal Imaging and Analysis Lab National Institute for Medical Research The Ridgeway Mill Hill London NW7 1AA Access Core funded facility by MRC, and shared freely by users within NIMR External users are charged to use the facility Expertise Live cell imaging, CARS, small animal MRI. Justin Molloy's course in light microscopy. User support, collaboration on biological imaging projects. Improving microscope performance, and development and implementation of advanced imaging techniques such as super resolution imaging, new probes, live cell/tissue imaging, correlative microscopy. Future requirements Imaging in tissues and whole animals, CLEM	Confocal microscopy Leica TCS SP1 upright Leica TCS SP2 upright Leica TCS SP2 AOBS inverted Leica TCS SP5 upright Leica TCS SP5 Multiphoton upright PerkinElmer UltraView LCI Spinning Disk Fluorescence imaging 2 X DeltaVision RT Live cell imaging microscope Inverted DeltaVision DV2 widefield fluorescence microscope
London, QMUL Ann Wheeler +44 (0)207 882 2406 Andy Bushby (EM) Gary Warnes (Flow cytometry) Cleo Bishop (High Content Screening) Blizard institute Advanced Light Microscopy facility Blizard Institute QMUL 4 Newark Street London E1 2AT Access Priority given to Blizard Institute users followed by QMUL then external External commercial and academic work as required DIY or full service available Expertise Core Facility. Super-resolution imaging Image Processing FRET/FRAP Tile-scanning of large areas	Confocal microscopy Zeiss LSM 710 META upright Zeiss LSM 510 invert with live cell imaging chamber Andor Spinning Disk system with FRAP and EMCCD camera Fluorescence imaging Zeiss Axiovision 200M inverted with Prior x,y,z stage Leica DM5000 with additional histology camera Leica DM4000 MBF Stereology system Zeiss AV200 PALM lasercapture/microdissection Leica MZ7s dissection microscope with colour camera Software Metamorph, Imaris, ImageJ + Fiji, Matlab, Cell Profiler Electron microscopy Carried out by the Nanovision centre Flow cytometry Carried out by the Flow cytometry facility High Content Screening InCell 1000 imaging system
London, UCL Jeremy Hebden +44 (0)207 679 0280 Biomedical Optics Research Laboratory Department of Medical Physics & Bioengineering University College London Malet Place Engineering Building London WC1E 6BT Access Highly variable, depending on demand Expertise Functional optical tomography of the neonatal brain. Real-time optical imaging of seizure in infants, including associated haemodynamic activity in the deep brain.	In vivo imaging systems 32-channel time-resolved optical tomography system 32-source, 16-detector optical topography system Lasers IMRA dual-wavelength fibre laser Fianium supercontinuum laser Ti:S picosecond las
London, UCL Andrew Vaughan (LM) +44 (0)207 679 7904 Jemima Burden (EM) +44 (0)207 679 3650 MRC Laboratory for Molecular Cell Biology Unit University College Gower Street London WC1E 6BT Access Mostly internal Small number of external users; about one or two a year Expertise Core facility. Collaboration and development of techniques, image processing and analysis EM facility - Preparation cell biological material for TEM and SEM. Cryo preparation techniques for biological samples (HPF and FS, and Tokuyasu) CLEM, 2D and 3D Future requirements Tools for image analysis and data processing	Light Microscopy Facility Confocal microscopy Leica TCS SP5 with DMI6000 Inverted with motorised stage and Pe-Con stage-top incubator 2 Leica TCS SPE with DM2500 Uprights Bio-Rad Radiance 2100 with Nikon E800 Upright Perkin Elmer Ultraview Vox spinning disc with Nikon Ti Inverted, Solent Scientific environmental chamber, Prior stage and piezo focus Time-lapse microscopy Zeiss Axiovert 135TV with LEP focus, stage, filters and shutters, environmental chamber for time-lapse imaging, Volocity software Zeiss Axiovert 200M with LEP stage and shutter, environmental chamber for time-lapse imaging, Volocity software Fluorescence microscopy Four Zeiss upright and one Leica inverted microscope/camera systems using Improvision Openlab capture software Software Packages Two Volocity 5 systems with all analysis packages. One 32-bit Mac and one 64-bit PC. One Metamorph 7.5 analysis station. Support for Leica LAS AF and Image J Electron Microscopy Facility Electron microscopy FEI Tecnai G2 Sprit TEM 120kV, with tomography stage and holder, side mount SIS Morada 11mpx digital camera FEI-Philips CM10 TEM with side mount Gatan 1kx1k digital camera Specimen preparation Leica High Pressure Freezer EMPACT 2 Leica AFS freeze substitution and low temperature embedding devices (1 AFS1, 1 AFS2 with FSP) Leica Ultracut E room temperature Leica Ultracut UCT room temperature Leica Ultracut UCT, FCS cryo Leica Knife maker Baltec Med020 dual gun sputter coater (carbon and carbon/platinum), rotary shadower, freeze drier, glow discharger Biorad Critical Point Drier 3000 Software SIS iTEM Inspect 3D Amira
London, UCL Chris Thrasivoulou +44 (0) 207 670 0904 Tim Robson Danial Ciantar Jane Pendjiky Centre for Cell & Molecular Dynamics UCL Faculty of Life Science Imaging Centre (Biosciences) 5th Floor Rockefeller Building 21 University Street London WC1E 6JJ Access All users welcome (Internal and External) Flat rate for all confocals £20 per hour for all users Overnight rate £10 per hour Expertise The unit has four experienced members of dedicated staff who are on hand at all times. Full training of all users at no extra cost. Free consultancy service available for help with experimental design and imaging, trouble-shooting requirements. Future requirements New microscopes will be added in the future to cater for research needs. This will include spinning disk confocals, resonant scanning confocals and Multiphoton-OPO systems for Second and Third Harmonic Generation microscopy that this unit is developing.	Confocal microscopy Leica SP2 UV upright multiphoton, FLIM live-cell imaging Leica SP2 fixed-stage upright, multiphoton, live-cell imaging Leica SP2 upright, single photon system Leica SP1 upright UV/ single photon system Leica SP1 upright single photon system Leica SP1 fixed-stage upright, single photon system Leica SPE (x2) upright, single photon system, live-cell imaging Leica SPE inverted, single photon system, live-cell imaging Olympus Flowview 1000, Sim-Scan, TIRF single photon system, live-cell imaging, ZDC Zeiss 510 upright/invert multiphoton, live-cell imaging Zeiss 510 META UV-VIS, inverted single photon system Zeiss 510 META inverted VIS, inverted single photon system Zeiss 700 inverted, single photon system Fluorescence Wide-Field Microscopy Zeiss AxioPlan 400 inverted live cell imaging with Orca camera ZeissAxioPlan2 upright, laser ablation, Orca camera Olympus IX81 inverted, live cell imaging with ZDC
London, UCL, Centre for Advanced Biomedical Imaging Mark Lythgoe +44 (0)20 7679 6164 Centre for Advanced Biomedical Imaging Paul O'Gorman Building University College London 72, Huntley Street London WC1E 6DD Access Internal and External commercial & external academic available DIY or Full service work Expertise Core Facility; In vivo Preclinical Imaging; In vivo Tumour, Cardiac and Neuro-Imaging; Optogenetics; Magnetic nanoparticles; Probe and MRI contracts agent development; Multimodal Image analysis; Brain atlas generation; In Vivo Experimental models (eg stroke and myocardial infarction)	In vivo Preclinical Imaging: 9.4T Magnetic Resonance Imaging: Agilent Technologies Photoacoustic Imaging: based upon the use of Fabry Perot polymer film sensing interferometers Ultrasound: A Vivid 7 Dimension (GE Healthcare) SPECT/CT: Bioscan system Bioluminescence and fluorescence imaging system: Biospace Lab PhotonIMAGER™ *CT-guided in vivo* irradiation:** Xstrahl system
London, UCL, ICH Bertrand Vernay +44 (0)2079052224/2715 Confocal Microscopy Core Facility UCL Institute of Child Health 30 Guildford Street London WC1N 1EH Access All users welcome (Internal and External) academic and commercial. Flat rate for all confocals £20 per hour for UCL users, overnight rate £10 per hour (please contact facility manager for external rates). DIY or full time support if required. Expertise Image acquisition of fixed and live samples with OpenLab, Volocity, Leica and Zeiss confocal softwares. Image processing and quantitative analysis with Volocity, ImageJ and CellProfiler. Help for manuscript preparation: Photoshop, Illustrator, InDesign	Confocal microscopy Leica SP2 UV-VIS upright, multiphoton excitation, live-cell imaging (inverted stand available), FRAP, FRET Zeiss 710 UV-VIS, inverted single photon system, live-cell imaging, FRAP, FRET, spectral imaging Widefield microscopy Zeiss Axiovert 135 Fluorescence widefield microscope, live cell imaging (OpenLab, Volocity acquisition software)
London, UCL, WIBR Tomas Adejumo Flow Cytometry and Confocal Microscopy Manager Tel1: +44 (0)207679 6719 Tel2: +44 (0)207679 6895 George Chennell Applications Specialist - Laser Micro-Dissection / Confocal Microscopy/ Live Cell Imaging/ Seahorse Bioanalyser Tel: +44 (0)207679 6757 Scientific Support Services UCL Cancer Institute and Wolfson Institute for Biomedical Research Gower Street UCL London WC1E 6BT Access Internal (75% on all instruments) External commercial and external academic (25% on all instruments) DIY or full service work Expertise Core facility; software analyses; High resolution imaging	Confocal microscopy Leica TCS SP PerkinElmer UltraView Volocity Acquisition Spinning Disk Fluorescence microscopy Zeiss CellObserver Live Cell Imaging Microscope Zeiss AxioImager A1 fluorescent microscope PALM laser capture/microdissection Flow cytometry Beckman Coulter CyAn flow cytometer Beckman Coulter MoFlo high-speed sterile cell sorter Software Volocity Visualisation and Quantification
Manchester Peter March +44 (0)161 275 1571 University of Manchester Bioimaging Facility Faculty of Life Sciences Michael Smith Building Oxford Road Manchester M13 9PT Access Internal (95% on all instruments) External commercial & external academic (5% on all) Mainly DIY or piloted Expertise Expertise in live cell imaging and particle tracking. High resolution imaging. Future requirements Faster live imaging and in vivo animal imaging	Confocal microscopy Leica SP5 Tandem Head (inverted) Leica SP5 MP (upright) Leica SP2 MP (inverted) Nikon C1 (upright) Nikon C1/TIRF (inverted) Nikon Swept Field Confocal (inverted) Widefield microscopy API Delta Visions MetaMorph based live cell system with FRAP Image Pro live cell imaging systems BD Pathway 855 high throughput system PALM laser microdissection
Manchester, Paterson Steve Bagley +44 (0)161 446 8631 Advanced Imaging Facility (CR UK) Paterson Institute for Cancer Research University of Manchester Wilmslow Road Manchester M20 9BX Access CR UK Internal 80% on all instruments CR UK external access 20% Expertise Core Facility. High mag/resolution low light time lapse, FRAP, FRET, photoactivation	Confocal microscopy Roper CSU22 spinning disk (invert) Fluorescence imaging EM CCD imaging (invert) Live cell imaging systems/chambers Deltavision Core (invert) Roper FRAP on the Fly system (invert) CRI Nuance Histology imaging Mirax Scan (300 slide Histology Scanner)
Norwich, John Innes Kim Findlay +44 (0)1603 450668 Grant Calder +44 (0)1603 450298 Peter Shaw +44 (0)1603 450330 BioImaging Facility Department of Cell and Developmental Biology John Innes Centre Norwich Research Park Norwich NR4 7UH Access Internal on all instruments External commercial & external academic (on application) DIY or Full service work Expertise Expertise in plant cell biology, in vivo imaging. High resolution cryo-SEM, GFP technology, General TEM sample prep and imaging Future requirements Access to super resolution, image analysis especially tools for shape recognition in organisms	Confocal microscopy Zeiss LSM 510 META upright Leica SP1 upright Leica SP2 invert Visitech QLC100 Spinning Disk Upright Fluorescence imaging Leica DFM6000 CCD imaging Zeiss PALM laser capture/micro dissection Live cell imaging chambers Electron microscopy FEI Tecnai 20 TEM FEI XL30 FEG SEM Zeiss Supra 55VP FEG SEM GATAN Cryosystems on both SEMs Deben Coolstage for Zeiss SEM
Norwich, UEA Paul Thomas +44 (0)1603 592196 Alba Warn +44 (0)1603 591776 Henry Wellcome Laboratory for Cell Imaging School of Biological Sciences University of East Anglia Norwich NR4 7TJ Access Full access to external users, though internal users get priority. DIY or Full service work Expertise Core Facility. Live-cell imaging (ion measurements and cell tracking); Image analysis (Intensity and ratiometric analysis, cell tracking, deconvolution, co-localization). Offers a 2-day course in live-cell imaging.	Confocal microscopy Zeiss LSM 510 META invert/upright Leica TCS SP2 invert LaVision Biotec TriM Scope II with TCSPC FLIM capability (invert/upright) Fluorescence imaging Zeiss Axiovert 200M with environmental chamber for variety of samples Zeiss Axiovert 200M with environmental chamber for 6- and 24-well plates Zeiss Axioplan 2ie for fixed specimens Zeiss SV11 stereomicroscope
Nottingham Tim Self +44 (0)115 8230090 Institute of Cell Signalling School of Biomedical Sciences University of Nottingham Queen's Medical Centre Nottingham NG7 2UH Access Training provided 60% ICS use Outside collaboration possible Expertise Live cell pharmacology, novel ligands. FCS Future requirements Analysis algorithms for HCS approaches	Confocal microscopy Zeiss LSM710 META invert with live cell perfusion system Zeiss LSM510uv META invert with live cell perfusion system Zeiss NLO Confocor3 META FLIM invert Zeiss Exciter invert live cell + TIRF Zeiss Confocor2 invert High Content Screening Molecular Devices Ultra confocal plate reader + robot Molecular Devices Micro widefield plate reader + environ
The Open University Heather Davies +44 (0)1908 653392 The Open University Electron Microscopy Suite Walton Hall Milton Keynes MK7 6AA Access Internal academic (university wide) External commercial and academic Can be used as DIY or with technical help	Electron microscopy JEM 2100 - a 200kV TEM from JEOL - high resolution EM/tomography, configured with a high tilt polepiece and specimen holder for tomography and has other analytical capabilities Zeiss Supra 55V - Analytical FEGSEM with variable pressure mode, enabling imaging of vacuum-sensitive and/or non-conductive samples without the need to apply conductive coating JEM 1400 - a 120kV TEM from JEOL JEM 1010 - a 100kV TEM from JEOL Extensive preparation facilities
Oxford Ian Dobbie +44 (0)1865 613323 Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU Access All instruments are available to internal users Currently no policy for external users	Confocal microscopy PerkinElmer Spinning disk Fluorescence imaging DeltaVision Core with FRAP module Olympus IX70 with micro-injection Other OMX Super-resolution microscope
Oxford c/o Chris Hawes School of Life Sciences Oxford Brookes University Gipsy Lane Oxford OX3 0BP Access Unit services the University External users welcome Training available Oxford Microscopy Consultancy offers a full microscopy service (microscopy@brookes.ac.uk) Expertise Live cell imaging, electron tomography, cryo-preparation Future requirements Spinning disc confocal, TIRF	Confocal microscopy Zeiss LSM 510 META upright Zeiss LSM 510 invert Fluorescence Zeiss Axioplan +CCD Electron Microscopy Hitach H-7650 120 kV tomographic TEM Hitachi S-3400N variable pressure SEM Preparative equipment Baltec HPM 010 high pressure freezer freeze-substitution RMC PowerTome PC with CR-X cryo attachment Flow cytometry Becton Dickinson FACSCalibur cytometer
Oxford, MRC Harwell Jeremy Sanderson +44 (0)1235 84 1051 (office) +44 (0)1235 84 1181/1037 (labs) Mammalian Genetics Unit MRC Harwell Harwell Science and Innovation Campus Didcot Oxfordshire OX11 0RD Access Internal on all instruments External contracted work by appointment	Confocal microscopy Zeiss 710 Laser Scanning Confocal Microscope Leica TCS SP5 Laser Scanning Confocal Microscope Multi-photon microscopy Zeiss 710 NLO on upright Axio-examiner Z.1 + BiG GaAsP detectors (on LSCM above) Fluorescence microscopy Olympus IX-81 inverted + XM10 camera Olympus BX-41 upright + CFW1312M camera Zeiss Axiophot2 upright + AxioCamHRc camera Leica DM2500 + Hamamatsu 9300 fast-frame camera Widefield microsopy Olympus BX-51 upright + DP25 digital colour camera Electron microscopy JEOL JSM-6010LV SEM Image analysis Perkin-Elmer Volocity 5
Pirbright, Institute for Animal Health Pippa Hawes +44 (0)1483 231103/231026 Bioimaging Institute for Animal Health Ash Road Pirbright Surrey GU24 0ND Access Internal (mainly) External academic or commercial collaboration possible Expertise Core Facility, containment microscopy Cell biology and virology Confocal and transmission electron microscopy Live cell confocal microscopy at SAP04 containment Future requirements TIRF, multi-photon, image analysis, full data management system	Confocal microscopy Leica SP5 (upright and invert) Leica SP2 (upright) Leica SP2 (invert, for live cell imaging at SAP04 containment) Vibrating microtome, cryostats, LMD's, fluorescent microscope, camera systems Electron microscopy FEI Tecnai 12 120kV TEM, with Tietz F214 camera Cyro-TEM and dual axis tomography holders Leica CPC plunge freezer Leica HPM100 HPF Leica AFS, AFS2 Leica UC6 ultramicrotome Leica UC7 cryo-ultramicrotome Software Inspect 3D, IMOD, Amira
Rothamsted Allison van de Meene +44 (0)1582 763 133 Rothamsted Bioimaging PPM Department Rothamsted Research Harpenden AL5 2JQ Access Internal and external, academic research groups and commercial Independent or full service work Expertise Core Facility. Plant cell biology. Live cell microscopy, sample preparation for sectioning, sectioning, cryoSEM, EDS, high resolution TEM, electron tomography and image analysis. Future requirements In vivo imaging and image analysis tools	Confocal microscopy Leica TCS SP1 inverted Widefield & fluorescence microscopy Zeiss upright and inverted microscopes with CCD imaging both in colour and monochrome Timelapse capabilities PALM laser capture/microdissection Leica M205 FA automated stereomicroscope Electron microscopy Jeol 6360 LVSEM with Gatan Alto 2100 cryosystem, Deben coolstage and Oxford EDS High resolution Jeol 6700F FEG-SEM with Gatan Alto 2500 cryosystem and Oxford EDS Jeol 2011F TEM with Gatan dual-axis holder for tomography, Gatan cryoholder and Oxford EDS Image Analysis Amira, MetaMorph, ImageJ
Sheffield Colin Gray +44 (0)114 2261234 Light Microscopy Core Facility Faculty of Medicine, Dentistry and Health The University of Sheffield Beech Hill Road Sheffield S10 2RX Access Internal on all instruments External commercial & academic on application DIY or full support service work Expertise Core facility, multiphoton, time lapse imaging	Confocal microscopy 1 Inverted multiphoton Zeiss LSM510 META with a Coherent Chameleon MP laser and environmental control 1 Upright multiphoton Zeiss LSM510 META with a Coherent Chameleon MP laser with dipping objectives and environmental control Becker and Hickl FLIM Live cell imaging Leica AF 6000LX with full environmental control Fluorecence microscopy Leica DMI 4000B Image Processing Central server with remote access and 2 offline workstations
Sheffield Darren Robinson +44 (0)114 2224682 Light Microscopy Facility University of Sheffield Alfred Denny Building Western Bank Sheffield S10 2TN Access Internal (90% on all instruments) External commercial & external academic (10% on all) Expertise Core facility	Confocal microscopy Zeiss LSM 510 invert Olympus FV1000 Upright with SIM scanner Leica SP1 PerkinElmer UltraView Spinning Disk Nikon A1 invert with TIRF Fluorescence imaging EM CCD live cell imaging Deltavision RT with quantifiable laser module High content Molecular Devices MetaExpress Micro
Sheffield Nicola Green +44 (0)114 2223932 Kroto Research Institute Confocal and Two-Photon Imaging Facility Kroto Research Institute North Campus University of Sheffield Broad Lane Sheffield S3 7HQ Access Internal External commercial & external academic DIY or with Experimental Officer support Expertise Core facility; Two-photon imaging of biological tissue	Confocal microscopy Zeiss LSM 510 META NLO invert with environmental chamber Zeiss LSM 510 META NLO upright (with in house modifications for time resolved emission microscopy) Fluorescence imaging Zeiss epifluorescent inverted microscope with environmental chamber
Southampton Anton Page +44 (0)23 8079 4815 Biomedical Imaging Unit Level B South Block MP12 General Hospital Southampton SO16 6YD Access All users welcome (Internal and External) Diagnostic work 50% (internal & external) Academic 50% (mostly internal). Independent or full service work. Commercial service available. Free instrument training of all users. Consultancy service to help with experimental design, imaging and analysis Expertise Joint University & NHS Trust core facility. The Unit has 5 experienced full time staff supporting research and diagnostic work. Staff support on hand at all times. Confocal microscopy, live cell imaging, TEM, SEM, x-ray microanalysis, diagnostic electron microscopy (renal, nerve, muscle etc), national centre for the diagnosis of primary ciliary dyskinesia. Art-science collaborations Artist in residence Training: One week TEM course (IBMS accredited) One week advanced LM course (IBMS accredited) Trainee biomedical scientists specialising in electron microscopy (graduate day-release) Future requirements 3D light and electron microscopy. Correlative light & electron microscopy. Low power confocal imaging. 3D rendering and visualisation software.	Confocal microscopy Leica SP2 Leica SP5 (Inverted, with live cell imaging & resonant scanner) Electron microscopy FEI Tecnai 12 TEM (with XRM) Hitachi H7000 TEM FEI Quanta 200 SEM (with XRM, variable pressure & environmental modes) Full TEM & SEM preparation facilities including cryosectioning (all independent or full service) Fluorescence imaging Olympus IX 81 (with live cell imaging) Zeiss Axioskop 2 Leica DM IRBE (with live cell imaging) Leica DM RBE Light microscopy Olympus DotSlide with remote access High speed video microscopy (with live cell imaging) Image analysis SIS iTEM, SIS Cell^P, Zeiss KS software
Southampton Hans Schuppe +44 (0)23 8059 4235 Imaging and Microscopy Centre School of Biological Sciences Life Sciences Building 85 University of Southampton Highfield Campus Southampton SO17 1BJ Access Open to all users (academic, non-academic) Internal (85% on all instruments) External commercial, external academic & external public (15% on all instruments) DIY or full service work Expertise Modern Core Facility with containment level 1 and 2 labs. Live cell imaging, Multiphoton microscopy, Fluorescence correlation spectroscopy (FCS), Electrophysiology. Future requirements Super-resolution fluorescence microscopy.	Confocal microscopy Leica TCS SP2 FCS multiphoton upright with environmental chamber and stage-top incubator Zeiss LSM 510 META upright with fixed stage and UV laser Fluorescence imaging Zeiss Axiophot 2 upright Zeiss Axioskop 2 upright with fixed stage Zeiss Axiovert 200 invert Leica DM IRB invert with Leitz micromanipulators Live cell imaging chambers Electrophysiology Axoclamp-2A amplifier Axopatch 200B amplifier Preparation room Wolf Lab. Galaxy S Incubator Microbiological Safety Cabinet Class II Leica MZ 16F stereomicroscope
Strathclyde Gail McConnell +44 (0)141 548 3895 Dave Blatchford + 44 (0)141 548 4694 Centre for Biophotonics Institute for Pharmacy and Biomedical Sciences University of Strathclyde 27 Taylor Street Glasgow G4 0NR Access Internal External commercial & external academic (20% on all) DIY only (following in-house training) Expertise Can offer CARS and expertise in optical trapping. Core Facility. Optical instrumention development and high-resolution optical imaging. Future requirements New tools for illumination and light sources	Confocal microscopy Bio-Rad 1024/Nikon Eclipse E600FN upright microsocpe Bio-Rad 1024/Nikon Eclispe TE300 inverted microscope Leica TCS SP5 with DM6000B upright microscope Multi-photon/confocal microscope Bio-Rad Radiance & Coherent Chameleon laser with Nikon Eclipse TE300 inverted Fluorescence imaging Upright Nikon E600F epi-fluorescence microsocpe Nikon TE300 inverted microscope with Solent Scientific environmental chamber, Sutter Scientific Lambda 12 controller Lasers Coherent Mira-F (2x: 150 fs, 700-920nm) Coherent Mira-D (150ps or 2ps, 700-920nm) Fianium 2W Yb:YAG laser (300fs, 1064nm) IPG Yb:YAG laser (10W, cw) Del Mar Cr:Forsterite laser with frequency doubling option (300mW & 120 fs @ 1240-1280nm or 100mW & 120 fs @ 620-640nm or 60 mW & 1.5 ps @ 620-640 nm) OPO I (~150 fs or ~2ps, 920-1180 nm) OPO II (~300 fs, 1380-1600nm) *Systems that may* become part of facility in due course - availability currently upon request:** CARS microscope based on Olympus FV300 & modified Olympus IX inverted platform Optical trapping & fluorescence system - suitable for live cell manipulation Olympus FV1000 with dual-scanning system/IX inverted microscope
York Peter O'Toole +44 (0)1904 328722 Jo Marrison +44 (0)1904 328720 Imaging and Cytometry Laboratory Technology Facility Department of Biology University of York York YO10 5DD Access Internal (80% on all instruments) External commercial & external academic (20% on all) DIY or Full service work Expertise Core Facility. Probe development and high resolution imaging. Offers a range of microscopy courses.	Confocal microscopy Zeiss LSM 780 META multiphoton invert Zeiss LSM 710 META invert Zeiss LSM 510 META invert Zeiss LSM 510 META upright Andor Revolution XD Spinning Disk Fluorescence imaging EM CCD imaging PALM laser capture/microdissection Live cell imaging chambers Electron microscopy FEI Tecnai 12 TEM Jeol 6490 SEM with cryosystem High resolution Jeol 7500F FEG SEM GATAN CL and Cryo Clairscope ASEM Flow cytometry 2 Beckman Coulter CyAn flow cytometers Beckman Coulter Astrios high-speed sterile cell sorter Apogee flow system A40

Institution

Equipment

Aberdeen

Kevin Mackenzie
+44 (0)1224 437447

Miep Helfrich
+44 (0)1224 437443

Microscopy and Cellular Imaging Facility
Histology and EM Facility
Institute of Medical Sciences
University of Aberdeen
Foresterhill
Aberdeen
AB25 2Z

Access

Internal on all instruments
External commercial & academic on application
DIY or Full service work

Expertise
Core Facility; Histology, Electron Microscopy, MicroCT

Confocal microscopy
Zeiss 700 Laser Scanning Confocal Microscope
Zeiss 710 Laser Scanning Confocal Microscope
Zeiss 510 META Laser Scanning Confocal Microscope
Fluorescence microscopy
Zeiss Imager M2 + Apotome
Zeiss Observer Z1
Zeiss PALM Laser Microdissection system
Calcium imaging
Improvision system for time-lapse imaging
Micro CT
SkyScan-1072 High-resolution desk-top micro-CT system
Live cell Imaging
Delta Vision Core wide field imaging system with EMCCD
Histology
Tissue processors, standard and motorized microtomes for wax and resin sectioning
Cryostat for frozen sectioning
Leica Bond-max fully automated immuno-stainer
Electron Microscopy
2x Philips CM10 TEMs with Gatan CCD digital imaging
Zeiss EVO MA10 SEM with Oxford Inca microanalysis
Leica UC6, Ultracut S & Ultracut E ultramicrotomes
Cryo Electron Microscopy
Leica Empact 2 High Pressure Freezer (with rapid transfer system)
Leica AFS (Automated Freeze Substitution) processor
Leica FC6 cryo-ultramicrotome

Belfast

Stewart Church
+44 (0)2890972274

Bioimaging Core Technology Unit
School of Medicine Dentistry and Biomedical Sciences
Queens University of Belfast
Medical Biology Centre
97 Lisburn Road
Belfast
BT9 7AB
Northern Ireland

Access

Internal on all instruments
External commercial & academic on application
DIY or full support service
Proof of principle/ developmental studies undertaken

Expertise
Core Facility, dedicated staff for each imaging platform. Computational methods for quantitative image analysis. Content Management Systems for all images, central server with remote access.
Workshops, training courses, teaching. Embedded Cat 2 facilities with microbiological safety cabinets and animal holding suite.

Confocal Microscopy
Leica TCS SP2 with DMRBE/DMIRE2
Leica TCS SP5 with DMI6000+Solent live cell chamber (Cat 2 facility)
Fluorescence Microscopy
Zeiss Axiovert 200 with Axiovision
Leica DMI6000B with FW4000
Live Cell Imaging
Zeiss Axiovert 200 with Eppendorf Injectman +Femtojet (Cat 2 facility)
Virtual Microscopy
Aperio CS Scanner
Aperio OS Scanner
Hamamatsu Nanozoomer
in vivo Imaging
Xenogen IVIS 200
FACS
BD FACSAria II (Cat 2 facility)

Birmingham, BALM

Manuel Forero (Imaging Specialist)
+44 (0)121 414 3392

Alicia Hidalgo
(co-director of BALM)
+44 (0)121 414 5416

Birmingham Advanced Light Microscopy Facility (BALM)
School of Biosciences
University of Birmingham
Edgbaston
Birmingham
B15 2TT

Access

Internal, mostly used by School of Biosciences
External academic: used by many other Schools within this University
External commercial subject to access charges
Can be used as DIY or with technical help

Expertise
Core facility, confocal microscopy.
Fixed, time-lapse and cell culture.
Computational methods for quantitative image analysis, object recognition and cell tracking.

Future requirements
Computational methods for imaging

Confocal microscopy
Leica SP2 AOBS inverted (4 lasers)
BioRad Radiance 2000 upright (3 lasers)

Birmingham, BALM-AWN

Joshua Rappoport

(co-director of BALM)
+44 (0)121 414 9019

Birmingham Advanced Light Microscopy - Advantage West Midlands Imaging Facility (BALM-AWM)
School of Biosciences
University of Birmingham
Edgbaston
Birmingham
B15 2TT

Access

Internal (university-wide)
External

Expertise
Core facility - Live cell imaging; Probe development and high resolution imaging

Confocal microscopy
Nikon A1R confocal/TIRF with spectral detector, EM-CCD, and Piezo-Z drive

Bristol

Mark Jepson (light microscopy)
+44 (0)117 33 12143

Paul Verkade (electron microscopy)
+44 (0)117 33 12179

Prof. Paul Martin
+44 (0)117 3312298

Wolfson Bioimaging Facility
School of Medical Sciences
University of Bristol
University Walk
Bristol
BS8 1TD

Access

Currently most use is Bristol-based and subject to access charges
Scope for external collaborations in speciality areas
We work on a recharge basis both for full service (EM) as well as DIY.

Expertise
Core facility.
CLEM - Correlative light and electron microscopy. Fully integrated Light and Electron Microscopy Unit. Live cell imaging (especially intracellular trafficking, model organisms, bacterial infection).

Future requirements
Tools for image analysis, especially tracking.

Confocal microscopy
4 Leica CLSM systems: inverted SP5 AOBS, SP2 AOBS, SP2; upright NT
Spinning disk confocal: Perkin Elmer UltraVIEW ERS 6FE
Wide-field microscopy
3 Leica AS-MDW
2 Leica microscopes with Hamamatsu cameras, automated filter wheels and shutters, Improvision Volocity software
TIRF (Leica AM TIRF MC system for total internal reflection fluorescence microscopy or wide-field imaging)
Electron microscopy
FEI Tecnai 12: 120kV transmission electron microscope
FEI Tecnai 20: 200 kV transmission electron microscope equipped with a STEM unit; suitable for cryo-EM and electron tomography, including STEM tomography.
FEI Quanta 400 variable pressure scanning electron microscope
Extensive EM preparation facilities.
Other
Offline image analysis suite for LM and EM.
Almost all Light Microscopes are equipped with live cell imaging chambers.
Cell culture facilities within the facility.

Cambridge

Richard Adams
+44 (0)1223 333782

Department of Physiology, Development and Neuroscience
University of Cambridge
Downing Street
Cambridge
CB2 3DY

Expertise
Time-lapse imaging, image processing, computational methods

Image analysis.
Cell tracking and cell shape analysis in whole embryos.
Computational analysis of tissue dynamics during embryogenesis.

Cambridge

Matt Wayland
+44 (0)1223 769282

Imaging Facility
Department of Zoology
University of Cambridge
Downing Street
Cambridge
CB2 3EJ

Access

Internal (80% on all instruments)
External commercial & external academic (20% on all)
DIY or full service work

Expertise
Core Facility
Image analysis and programming

Confocal microscopy
Leica SP5 invert
Leica SP5 upright
Leica SP upright
UltraView CSU-10 Spinning Disk
MicroPoint laser ablation / microdissection
Live cell imaging chamber on SP5 invert (up to 37^oC and 5% CO2

Cambridge

Alexandre Kabla
+44 (0)1223 766208

Department of Engineering
University of Cambridge
Trumpington Street
Cambridge
CB2 1PZ

Expertise
Imaging, computational methods

Image Analysis
Image processing and mechanical properties of multicellular assemblies

Cambridge

Clemens Kaminski
+44 (0)1223 763135

Laser Analytics Group
Department of Chemical Engineering and Biotechnology
University of Cambridge
Pembroke Street
Cambridge
CB2 3RA

Access

Internal
External
DIY

Expertise
All aspects of quantitative multidimensional microscopy

Confocal microscopy
Custom built widefield FLIM platform with LED and Supercontinuum excitation (430-700 nm). Both ultrahigh speed FLIM (15 fps at full field) and excitation at multiple modulation frequencies are possible.
Custom designed hyperspectral imaging confocal station, with supercontinuum excitation (430-700 nm excitation / emission, at electronically selectable, arbitrary wavelength combinations). Full resolution of fluoresence excitation and emission spectrum, fluorescence lifetime and polarisation state. TCSPC, Fluorescence Anisotropy Imaging
Leica SP5 confocal platform with resonant scanner.

Cambridge

David Klenerman
+44 (0)1223 336481

The University Chemical Laboratory
Dept of Chemistry
University of Cambridge
Lensfield Road
Cambridge
CB2 1EW

Expertise
Laser fluorescence spectroscopy and scanning probe microscopy

Fluorescence microscopy
Two colour total internal reflection single molecule fluorescence microscope

Cambridge

Jeremy Skepper
+44 (0)1223 333774

Janet Powell
+44 (0)1223 333774

Multi-Imaging Centre
Anatomy Building
Physiology Development and Neuroscience,
University of Cambridge
Downing Site
Cambridge
CB2 3DY

Access

Internal
External
Commercial.
DIY and service work.

Expertise
Specimen preparation, ambient and low temperature, stereology, x-ray microanalysis, immunocytochemistry, bio-materials microscopy, physiological and time-lapse microscopy.

Confocal microscopy and LMD
Leica SP1- multiphoton (invert)
Leica SP1-UV (invert)
Leica SP2-405 - 633 (invert)
Leica SP2-456 - 633 (invert)
Leica SP5-456 - 633 (invert physiology)
Leica LMD 6000 (upright laser microdissection microscope)
Electron microscopy
FEI Technai G2 200 kv, STEM, tomgraphy, cryo-transfer, HAADF, SAED, CBED, EDAX Genesis EDX, Deben AMT digital camera
FEI-Philips XL30 FEGSEM, SE and BSE detectors
Oxford INCA EDX with 30mm pentafet ATW detector
Quorum Emitech PP2000 cryotransfer system
Specimen preparation
Leica VT1000S vibrating blade microtome
Leica CM 3050S Cryostat
Leica CPC plunge and impact freezing device
2 x Leica AFS freeze substitution and low temperature embedding devices
3 x Leica Ultracut E
Leica Ultracut UCT
Leica Ultracut S and FCS cryosectioning device
Edwards 306 auto, Carbon coater, freeze drier and glow discharge unit
Quorum Emitech K575X dual head Au and Cr sputter coater and remote pumping station for TEM cryo-transfer specimen holder
Polaron CPD

Cambridge, Babraham

Simon Walker
+44(0)1223 496618

Imaging Facility
The Babraham Institute
Babraham
Cambridge
CB22 3AT

Access

Full access to external users, though internal users get priority.
DIY or Full service work

Expertise
Core Facility
Live cell fluorescence imaging

Confocal microscopy
Zeiss LSM 510 META invert
Olympus FV1000 invert (x2)
Spinning Disk with EM CCD (Andor)
Fluorescence imaging
Olympus CellR (widefield+TIRF)
Live cell imaging chamber
High Content Screening
BD Pathway 855
MetaSystems Metafer / MetaCyte

Cambridge, Centre for Stem Cell Research

Peter Humphreys (light microscopy)
+44(0)1223 760200

Rachael Walker (flow cytometry)
+44(0)1223 760227

Centre For Stem Cell Research
Tennis court Road
Cambridge
CB2 1QR

Expertise
Core Facility, image analysis

Future requirements
HE/fluorescent upright
Plate scanning microscope

Confocal Microscopy
Leica SP5 invert + live cell chamber x2
Andor Revolution XD spinning disk
Widefield and cell culture
Nikon Biostation IM
Essen Incucyte HD
Essen Incucyte
Zeiss AxioExaminer
Leica DMI4000
Olympus IX51 x3
Flow Cytometry
MoFlo
CyAN

Cambridge, Gurdon Inst.

Alex Sossick
+44 (0)1223 334082

Wellcome Trust/Cancer Research UK Gurdon Institute
University of Cambridge
Tennis Court Road
Cambridge
CB2 1QN

Access

Priority given to Gurdon Institute users
Limited outside access

Expertise
Core Facility. Immunofluorescence; widefield epifluorescence and confocal microscopy, deconvolution

Confocal microscopy
Confocal scanning laser microscopes and multi-photon system
Widefield deconvolution and high throughput systems

Cardiff

Jim Ralphs
+44 (0)2920 874954

Tony Hayes
+44 (0)2920 876611

Marc Isaacs
+44 (0)2920 876611

Confocal Microscopy Unit
Cardiff School of Biosciences
Cardiff University
Cardiff
CF10 3AX

Access

Internal (mainly), but also external commercial & academic

Expertise
Core Facility; Immunofluorescence; Widefield epifluorescence and confocal microscopy

Confocal microscopy
Leica TCS SP2 AOBS laser scanning confocal microscope. Twin microscope configuration comprising upright (DM6000) and inverted (DMIRE2) stands (laser lines: 405, 458, 476, 488, 514, 543 & 633nm), galvonometer & electromotor stages; Solent Scientific incubation chamber with CO2/humidity and temperature regulators for in vitro work; brightfield, fluorescence, DIC and polarizing optics
Fluorescence imaging
Olympus BX series microscopes with fluorescence, DIC and brightfield optics refer to web pages for further information

Cardiff

Anna Hurley
+44 (0)2920 687004

Charlotte James
+44 (0)2920 687004

Central Biotechnology Services
Henry Wellcome Building
School Of Medicine
Cardiff University
Heath Park
Cardiff
CF14 4XN

Access

Internal and external users
Academica and Industry
User access or managed service

Expertise
Core facility, academic consultancy, certified to ISO 9001 and ISO 17025

Confocal microscopy
Leica SP5
Leica SP2
Flow cytometry
3 x FACS Calibur 4 colour
1 x Beckman Cyan ADP 9 colour
1 x FACS Canto II 8 colour
1 x FACS Calibur 3 colour
Beckman MoFlo - 9 parameters simultaneously using 6 colours

Cardiff

Jan Hobot
+44 (0) 2920 742131

Chris von Ruhland
+44 (0) 2920 742132

Medical Microscopy Sciences
School of Medicine
Cardiff University
Heath Park
Cardiff
CF14 4XN

Access

Internal and external both academic and commercial.

Expertise
Core facility.
Specimen preparation at ambient or low temperature for structural or immunocytochemical studies.
First-hand knowledge of modern acrylics (Lowicryls and LR White).
Tokuyasu or cryosubstitution methodologies.
Histology and LM preparations.
TEM and SEM X-ray microanalysis.
UKAS accreditation for X-ray microanalysis of materials and particulates for detecting asbestos.

Fluorescence imaging
Olympus BX51 Upright with Zeiss AxioCam camera.
Olympus CK40 Inverted with Polaroid digital camera.
Reichert Polarising Microscope.
Electron microscopy
Philips CM12 TEM with SIS MegaView III camera and EDAX Genesis X-ray microananalysis system.
JEOL 840A SEM with ADDA SIS digital imaging and INCA x-sight detector X-ray microanalysis system with elemental mapping plus Oxford CT1500 Cryosystem and stage.
Specimen Preparation
Leica EMTP Tissue Processor.
Reichert KF80 metal mirror/plunge freezer.
Dedicated Histology room.

Didcot, STFC

David Clarke
+44 (0)1235 567035

Octopus Imaging Cluster (Lasers for Science Facility)
Science & Technology Facilities Council
Rutherford Appleton Laboratory
Didcot
OX11 0QX

Access

Via peer-review panel for external and internal users
Approx 80% of scheduled time for long-term (>1 year) programmes, and 20% for proof-of-principle work
Additional access for users of other facilities (Diamond Light Source, ISIS, Research Complex at Harwell)
Commercial access also available

Expertise
Core Facility; Single molecule techniques, including in live cells; single molecule and ensemble FRET; FLIM; development of advanced laser microscopy instrumentation.

Future requirements
Image analysis for single molecule work

Central laser cluster
(ns, ps, fs and steady state lasers UV-near IR) feeding the following microscopy stations:
Single molecule microscopy
Multidimensional (simultaneous two-colour(FRET)/two polarisations) single molecule TIRF microscope (live cell and immobilised samples).
3-colour single molecule TIRF X-Y-t tracking (Multiprotein complex kinetics and dynamics).
5-colour single molecule TIRF X-Y-t tracking (Multiprotein complex kinetics and dynamics) - under construction - to be scheduled next year.
Confocal microscopy
Multiphoton confocal fluorescence microscope for FRET/FLIM Imaging.
Multicolour confocal fluorescence microscope with Becker & Hickl Fluorescence Lifetime Imaging (FLIM) module.
Support Equipment
Laser tweezers, steady-state and time-resolved laser spectroscopy apparatus, cell biology and biochemistry support labs

Dundee

Jason Swedlow
+44 (0)1382 385819

Swedlow lab
Wellcome Trust Centre for Gene Regulation & Expression
College of Life Sciences
MSI/WTB/JBC Complex
University of Dundee
Dow Street
Dundee
DD1 5EH

Open Microscopy Environment

Expertise
Structured illumination microscopy on OMX, expertise on high content screening and live cell imaging, development of data management tools and specifications for microscopy.

Future requirements
Money for meetings and travel to other sites for access and sharing of info and smart, programmable microscopes.

Dundee

Sam Swift
+44 (0)138 238 6412

Central Microscopy Facility
College of Life Sciences
MSI/WTB Complex
University of Dundee
Dow Street
Dundee
DD1 5EP

Access

User training/DIY for light microscopy
DIY or Full Service for EM
External academic / industry welcome by appointment

Expertise
Core facility; High resolution imaging, live cell imaging, FLIM, FRET, FRAP, immuno EM etc

Light Microscopy
BioRad Radicance 2100MP confocal / multiphoton
Zeiss LSM 510 META
Leica SP2
Becker and Hickl TCSPC FLIM
DeltaVision Personal, RT and Core restoration microscopes with EMCCD, Live Cell Chambers and Laser Modules
Nikon TIRF
OMX Structured Illumination Microscope
Electron Microscopy
FEI Tecnai 12 TEM
Jeol 1200 EX TEM
Hitachi s4700 FESEM

Dundee

Markus Posch (SULSA Scientific Officer for OMX)
+44 (0)138 238 6414

OMX Super-Resolution Microscopy
College of Life Sciences
University of Dundee
Dow Street
Dundee
DD1 5EH

Access

Any users welcome
Euro BioImaging Proof of Concept site(Advanced Light Microscopy - General Access)

Expertise
Structured Illumination Spatial Super-Resolution - Superfast Live Imaging

OMX ver. 3 platform (Applied Precision)

Durham

Tim Hawkins (Advance Light Microscopy)
+44 (0)191 334 1333

Christine Richardson (Electron Microscopy)
+44 (0)191 334 1285

Microscopy and Bioimaging Facility
School of Biological & Biomedical Sciences
Durham University
Durham
DH1 3LE

Access

Internal on all instruments
External commercial & external academic
DIY or Full Service

Expertise
Core facility; High resolution imaging, FRET, FRAP, FLIP. 5D live cell imaging, TIRF, photoactivation/conversion,
ImmunoGold, Cryosectioning, Ultramicrotomy, Sterology.
Licenced to image plant:pathogen interactions within facility.
Collaboration & developments of new techniques (eg photonics, chemical probes)

Future requirements
SuperResolution, FCCS & FLIM

Confocal microscopy
Leica TCS SP5 Tandem head AOBS Invert (Resonant scanner)
Zeiss LSM 510 META invert
BioRad Microradiance Upright
Andor Revolution XD Spinning Disk Invert
Fluorescence Imaging
Leica MC TIRF Microscope
Zeiss Apotome Microscope
Zeiss Live Cell Imaging System
EM CCD Imaging
Most of the above microscopes are within live cell imaging/environmental chambers
Microinjection
Upright (DM2500) and inverted (DMI3000) DIC microscopes + multiple injection systems to work with a wide variety of organisms and tissues. HD imaging system.
Electron Microscopy
Hitachi H7600 TEM
High resolution Hitachi S5200 FESEM + Cryostage
Image analysis
Suite of high-end 64-bit PC workstations
Deconvolution
Sample Preparation
Leica High Pressure Freezer EMPACT
Leica AFS freeze substitution and low temperature
Leica Ultracut S room temperature
Leica FC6 cryo-ultramicrotome
Leica Knife maker
Baltec Critical Point Drier
Cressington coating units (Carbon, platinum, chromium, tungsten)
Leica CPC cryo workstation
Leica IGL. Automatic immunogold labeller
Leica MM80E metal mirror/plunge freezer
Tissue processing
Cryostat for frozen sectioning
Microtomes for wax and resin sectioning
Leica vibrating diamond knife

Edinburgh

Rolly Wiegand (LM)
+44 (0)131 650 2864
CALM - Confocal and Advanced Light Microscopy Facility

Shonna Johnston (Flow)
+44 (0)131 242 6567

Flow Cytometry Facility
Queen's Medical Research Institute
47 Little France Crescent
Edinburgh
EH16 4TJ

Access

Internal for University
External
DIY or fully assisted image acquisition

Flow

Internal for University only
Fully assisted cell sorting

Expertise
Core Facility; Teaching - Microscopy course (twice a year), high resolution cellular microscopy, image restoration and quantitation, live cell imaging

Future requirements
Hardware for deep penetration in/ex vivo live cell imaging and TIRFM

Confocal microscopy
Zeiss LSM 510 META, inverted
Leica SP5, inverted, both eqipped with environmental control
Fluorescence imaging
Olympus Cell P, inverted
Environmental control for live cell imaging
Optical Projection Tomography (Bioptonics 3001)
Image analysis
High-end workstations with SVI Huygens Essential, ImageJ and PE Volocity

Flow cytometry
Cytometry analysers
BD FACSCalibur
BD FACScan
BD FACSArray
Cell sorters
BD FACSVantage SE (Diva)
BD LSR Fortessa
BD FACSAria II

Edinburgh, Clinical Research Imaging Centre (CRIC)

Edwin J. R. van Beek
+44 (0)131 242 7760
SYNAPSE Chair of Clinical Radiology

Stuart Docherty
+44 (0)131 242 7767

Clinical Research Imaging Centre
University of Edinburgh
CO.19
Queen's Medical Research Institute
47 Little France Crescent
Edinburgh
EH16 4TJ

Access

Access through applications process
PET-CT 70% NHS service
MDCT 50% NHS service
MRI 100% research

Expertise
Core facility.
Imaging-based assessment of structure, function and treatment efficacy

Imaging
3T MRI (Siemens Verio) with fMRI, multinuclear spectroscopy and MR Elastography
Mock MRI system
320 MDCT (Toshiba Aquilion ONE)
128 mCT-PET (Siemens)
PET-trace 8 cyclotron (GE)
Radiochemistry
4 GMP hot cells and 3 research hot cells

Image analysis laboratory

Edinburgh, Orthopaedic Engineering Collaboration

Antonello Spadaccino
+44 (0)131 242 6295

Orthopaedic Engineering Collaboration
Chancellor's Building
University of Edinburgh
Room FU 413
49 Little France Crescent
Edinburgh
EH16 4SB

Access

Internal (75%)
External and commercial (25%)
Full service work
Induction training and supported DIY

Expertise
Scan and production of 3D images from samples.
Comprehensive tensile, 3 & 4 point bend tests.
Torsional testing, ultimate strength, fatigue and step loading. Combination of axial and torsional tests.

Xray Imaging
Skyscan 1172
Mechanical Testing
Zwick/Roel z0005

Edinburgh, IMPACT

Trudi Gillespie
+44 (0)131 6503278

IMPACT Imaging Facility
Hugh Robson Building
George Square
Edinburgh
EH8 9XD

Access

Internal for University
External
DIY or fully assisted image acquisition

Expertise
Core Facility.
High resolution cellular microscopy, image restoration and quantitation, live cell imaging, TRIF and FLIM

Future requirements
MP system for in/ex vivo live cell imaging

Confocal microscopy
2 Zeiss LSM 510 one inverted and one upright both coupled to a Coherent Mira mode-locked Ti:Sapphire multi-photon laser equipped with environmental control and a Becker and Hickl unit for fluorescence lifetime imaging (FLIM).
Leica TCS-NT, inverted and upright with environmental control
Fluorescence imaging
Olympus Cell R Dual TIRF System, with environmental control for live cell imaging
Image analysis
High-end workstations with SVI Huygens Essential and Pro, ImageJ, Imaris and PE Volocity

Edinburgh, MRC

Mike Millar
+44 (0)131 242 9155

Histology and Imaging Facility
MRC, Human Reproductive Sciences Unit
The Queens Medical Research Institute
New RIE
47 Little France Cres
Edinburgh
EH16 4TJ

Access

Internal on all instruments
External academic on application
DIY or Full service work

Expertise
Core facility.
Confocal microscopy, Stereology and immunohistology with emphasis on multi same species colocalisations.
Reproductive morphology

Confocal microscopy
Zeiss 510 Laser Scanning Confocal Microscope
Zeiss 510 META Laser Scanning Confocal Microscope
Zeiss 710 Laser Scanning Confocal Microscope
Widefield microscopy
Assorted upright and invert research grade microscopes with CCD imaging both in colour and monochrome
Live cell Imaging
Stereology using Media Cybernetics Image Pro Plus with stereologer plug in
Image Analysis
Huygens deconvolution software
Volocity 3D imaging software
Media Cybernetics Image Pro Plus
Histology
Tissue processors, standard and motorized microtomes for wax and resin sectioning
Cryostat for frozen sectioning
Leica Bond- fully automated immuno-stainer
Intavis whole mount in-situ hybridisation and immunostaining robot

Edinburgh, Brain Research Imaging Centre (BRIC)

Joanna Wardlaw (Director of Centre)
+44 (0)131 537 2943 (PA)

Duncan Martin (Scientific Business Manager)
+44 (0)131 537 2664

Brain Research Imaging Centre
University of Edinburgh
Division of Clinical Neurosciences
Bramwell Dott Building
Western General Hospital
Edinburgh
EH4 2XU

Access

Access through an application process
Research and commercial scanning work can be undertaken

Expertise
Stroke, FMRI, Diffusion and tractography, Perfusion imaging, Aging, Spectroscopy, Psychiatric disorders, Neurodegenerative disorders

1.5T (GE) MRI imaging capabilities
Structural
Diffusion and Tractography
Functional MRI
(Simultaneous EEG)
Perfusion and Bloodflow
Permeability
Spectroscopy
Temperature Mapping

Radiological advice on imaging and study protocols
Radiological interpretation of scans

Image Analysis methods

MRI Quality Assurance methods

Software tools for image analysis and image management

Essex

Philippe Laissue
+44 (0)1206 872246

Bioimaging Facility
Department of Biological Sciences
Wivenhoe Park
University of Essex
Colchester
CO4 3SQ

Access

Internal on all instruments
External commercial & academic on application; Nikon reference site
Proof of principle studies undertaken

Expertise
Core facility, rapid and long term live cell imaging, immunofluorescence, colocalisation, visualisation. Development of specialised instruments and computational methods for quantitative image analysis.

Future requirements
Further in-house development of instruments and algorithms. Super-resolution and FLIM.

Confocal microscopy
Nikon A1R (inverted) with 6 laser lines, hybrid scanner, spectral detector, automated XY and piezo Z,
TIRF/OAF/widefield with EM-CCD, live cell chamber
Bio-Rad Radiance 2000 with Olympus IX70 (inverted)
Widefield fluorescence Microscopy
Nikon Ti-E (inverted) with LEDs and Intensilight, EM-CCD, automated XY and piezo Z, live cell chamber
In-house developed TIRF and OAF instrument (inverted) with EM-CCD and triple split (Kad group)
Various upright and inverted fluorescence and brightfield microscopes with EM-CCD and colour CCD cameras
Several commercial and in-house developed imaging systems for measurement of photosynthesis by gas exchange and fluorescence, both in the laboratory and in the field (Lawson group).
Image Analysis
High end 64-bit workstations for offline image processing and analysis. NIS-Elements, deconvolution. Custom software using ImageJ and Matlab. Workshops, training courses, teaching. Extensive website for booking, training, resources.
Other
Fully equiped Cat 2 laboratory, cell culture facilities

Flow Cytometry
BD FACS Aria
BD FACS Calibur

Glasgow, Beatson

Kurt Anderson
+44 (0)141 330 2864

Beatson Institute for Cancer Research
Garscube Estate
Switchback Road
Glasgow
G61 1BD

Access

Priority given to Beatson users, followed by Glasgow University
Outside access primarily on a collaborative basis

Expertise
Live cell imaging, including time and frequency domain FLIM, TIRF, photo-activation / -bleaching / -conversion.
Mouse in vivo imaging, including collagen SHG and use of photo-activation and -bleaching, FLIM and FRET.

Future requirements
Image analysis, adaptive optics

Confocal microscopy
Olympus FV1000 (x2)
Nikon A1R
Leica TCS SP2
Zeiss LSM 710

FLIM
LaVision Bio-Tec TRIM scope
Lambert Instruments custom Spinning Disk
Lambert Instruments custom TIRF
Leica TCS SP2 with Becker & Hickle TCSPS 730

Live Cell
Custom TIRF
Olympus / Volocity Deconvolution system
Micro-injection
Medium Throughput Long Term Time Lapse (x4)

Other
Olympus OV100
Leica AF6000 micro-dissection
FacsAria
Upright stands for histology and immunofluorescence (x4)
Off-line image analysis (x8)

Leeds

Gareth Howell
+44 (0)113 3437270

Leeds Bioimaging and Flow Cytometry Facility
Faculty of Biological Sciences
University of Leeds
Leeds
LS2 9JT

Access

Internal and external, academic research groups and commercial

Expertise
Core Facility.
Collaboration and development of techniques, image processing and analysis. Live cell imaging.

Confocal microscopy
Zeiss LSM 510 META invert with motorised stage and 405 laser diode
Zeiss LSM 510 META upright with motorised stage
Fluorescence imaging
Delta Vision Deconvolution System
Live cell imaging chambers
Microinjection
Eppendorff FemtoJet system on an inverted Zeiss Axiovert 200
Flow cytometry
FACSCalibur 4 colour analyser flow cytometer
FACSAria 7 colour cell sorter
Partec PASIII 4 colour flow cytometer

Leicester

Kees Straatman
+ 44 (0)116 229 7085

Advanced Imaging Facilities
Department of Biochemistry
Henry Wellcome Building
University of Leicester
Lancaster Road
Leicester
LE1 9HN

Access

Mostly internal users but external users are welcome

Expertise
Core Facility.
Immunofluorescence, widefield epifluorescence, confocal microscopy, live cell imaging, microinjection, FRAP/FLIP/FRET, spectral imaging, high content screening/analysis.
Data analysis and quantification: Imaris, Volocity, Openlab, Huygens Essential deconvolution, ScanR/CellR, ImageJ, NIS Elements.
Teaching: Microscopy workshops, lectures

Future requirements
Technical support
Image indexing software
FCS/FLIM
Super resolution imaging
Whole animal imaging (funding secured)

Most systems with incubators, temp and CO₂ control
Confocal microscopy
Leica TCS SP5 inverted
Olympus FV1000 inverted
Nikon C1Si inverted
Zeiss LSM 7 MP upright (soon)
Fluorescence imaging
3 Nikon TE 300 inverted
1 Nikon Ti-E EM-CCD + LEDs inverted
Olympus CellR/scanR inverted
1 Nikon TIRF EM-CCD inverted
Olympus BX61 upright with Applied Spectral Imaging's scanning platform
Bioluminescence imaging
Olympus LV200 EM-CCD
Flow cytometry
BD FACS Canto II
Imagers
Typhoon Trio+
LAS4000

London, Imperial

Raffaella Carzaniga
+44 (0)207 594 5194

Electron Microscopy Centre
Imperial College
Exhibition Road
South Kensington
Biochemistry Building- Room 022
London
SW7 2AZ

Access

Internal and external academic research groups and commercial

Expertise
Cryo-Preparation methods for TEM, TEM for Cellular Biology & Single Particles.
Image processing for Single Particles

Electron microscopy
TEM-Philips CM100 (Tungsten electron source) with film plate
TEM- FEI Tecnai 12 (LaB6 electron source); CCD camera
TEM Philips CM200 (FEG electron source); CCD camera
TEM- Philips CM300 (FEG electron source); Helium cooled-Polara Stage; CCD camera
Vitrobot
HPF- High Pressure Freezing apparatus (EMPACT 2 with RT, Leica)
Ultramicrotomes (for ambient and low temperatures)
Parallel computer farm

London, Imperial

Dirk Dormann
+44 (0)208 383 8528

Microscopy Facility
MRC Clinical Sciences Centre
Faculty of Medicine Imperial College London
Hammersmith Hospital Campus
Du Cane Road
London
W12 0NN

Access

Core funded by MRC, only MRC users

Expertise
Core facility.
Quantitative image analysis, time-lapse

Confocal Microscopy
Leica SP1 invert
Leica SP2 invert
Leica SP5 invert with tandem scanner and FCS/FCCS
PerkinElmer UltraView Spinning Disk
Fluorescence imaging
Deltavision Spectris
Leica DMIRE2 inverted with Eppendorf microinjection system
Leica DMRB upright
Zeiss Axiovert 100S TV inverted
Zeiss Axiophot upright
Olympus SMZ-16X stereo microscope
PALM laser capture/microdissection

London, Imperial

Paul French
+44 (0)207 594 7713

Photonics Group
Physics Department
Imperial College London
South Kensington Campus
London
SW7 2AZ

Access

By collaboration

Expertise
Optical Microscopy, Optical Tomography, Optical Endoscopy, Imaging Data Management and Integration, Optical Image Analysis

Future requirements
Multidisciplinary training at the interface of photonics technology and life science applications.
Long term funding for core instrumentation staff.
Tb scale optical image data management systems.
Software tools including automatic image segmentation and multidimensional analysis.

Multidimensional fluorometer
Confocal/Multiphoton Microscope, Ti-Sapphire laser or supercontinuum excitation source/FLIM/polarisation-resolved imaging
Line-scanning semiconfocal microscope with Ti-Sapphire laser or supercontinuum excitation source
TIRF microscope, multispectral imaging, FLIM/polarisation resolved imaging capability
Wide-field FLIM microscope optimised for UV excitation
Multibeam multiphoton microscope (LaVision TriMScope) with FLIM
High throughput automated optically sectioned (Nipkow) FLIM microscope
High speed Nipkow disc FLIM microscope with supercontinuum excitation
STED FLIM microscope, Leica SP2 laser scanning confocal, Ti:sapphire laser
Wide-field FLIM endoscope/macroscope system
Microconfocal FLIM endoscope
FLIM Optical Projection Tomography (OPT)

London, Imperial

Martin Spitaler
+44(0)207 594 2023

FILM (Facility for Imaging by Light Microscopy)
Imperial College Exhibition Road
South Kensington / SAFB
London
SW7 2AZ

Access

Internal and external (academic and commercial)
DIY or assisted microscopy
Assay development

Expertise
Core facility

Confocal microscopy
Zeiss LSM-510
Leica SP2
Leica SP5 resonant
Leica SP5 MP-Flim upright and inverted
Widefield microscopy
EM-CCD
Incubation chambers
Automated XYZT imaging
Low and medium throughput
TIRF
Image analysis
Volocity
SimplePCI
Autoquant
CellProfiler
Matlab
Definiens

London, King's

Rainer Heintzmann

Matthias Gautel

Randall Division of Cell and Molecular Biophysics
King's College London
New Hunt's House
Guy's Campus
London
SE1 1UL

Access

Internal
Collaborations to apply superresolution imaging are possible
DIY

Expertise
Superresolution Microscopy.
Facilities for structured illumination, 4pi, STED (soon), expertise in deep tissue imaging, FRET and FLIM.

Future requirements
Financial support for personnel for applying existing expertise to critical biological problems

Structured Illumination
Lecia 4Pi TCS SP2
Leica STED

London, King's

Christopher Healy
+44 (0)207 188 1795

Tissue Imaging Centre,
Dept of Craniofacial Development,
King's College London Dental Institute at
Guy's,
King's College and St Thomas' Hospitals,
Floor 27 Tower Wing,
Guy's Hospital,
St. Thomas St.,
LONDON
SE1 9RT

Access

Internal (100% on microscopes, 90% on microCT)
External commercial & external academic (10% on all)
DIY on microscopes.
Part or Full service work on MicroCT and OPT

Expertise
Core facility.
Morphometrics, bone histomorphometry and high resolution imaging.

Confocal microscopy
Leica SP5 upright confocal microscope
Fluorescence imaging
Zeiss Axioskops with Axiocamera
PALM laser capture/microdissection
Live cell imaging chambers
microCT
GE Healthcare Locus SP specimen microCT
Optical Projection Tomography
Bioptonics 3001

London, CRUK-LRI

Daniel Zicha (LM)
+44 (0)207 269 3563

Lucy Collinson (EM)
+44 (0)207 269 3416

Anne Vaahtokari (Super-resolution microscopy)
+44 (0)207 269 3511

Cancer Research UK, London Research Institute
Lincoln's Inn Fields Laboratories
44 Lincoln's Inn Fields
London
WC2A 3PX

Access

LM - Priority given to LRI users, limited outside access
EM - Core Technology Facility, internal groups only
Super resolution - Priority given to LRI users, limited outside access

Expertise
Core facility.
Expertise in image processing and simulation.
Quantitative analysis with multi-modal microscopy.
EM - Preparation of cell biological material for TEM and SEM.
Cryo preparation techniques for biological samples (HPF, FS, Tokuyasu, freeze fracture, freeze drying, cryoEM)
Tomography
Correlative Light/ Electron Microscopy (CLEM)
Volume imaging (FIB/SEM, SBF/SEM, MicroCT, NanoCT)
Super-resolution microscopy

Future requirements
Support for developing new image processing approaches

Confocal microscopy
LSM 510 (Zeiss) including a multiphoton system
Leica SP5
Low light level imaging systems
Universal Imaging Metamorph,
Kinetic Imaging AQM
Molecular Devices Discovery-1
PerkinElmer UltraVIEW confocal imaging systems
Eppendorf Microinjection systems

Electron Microscopy Unit
Electron microscopy
FEI Tecnai G2 Sprit TEM 120kV, with tomography stage, single and dual tilt tomography holders, bottom mount Gatan Ultrascan CCD, side mount Gatan Orius CCD
FEI Tecnai G2 Sprit TEM 120kV, with cryo stage and cryo holder, bottom mount Gatan Ultrascan CCD, side mount Gatan Orius CCD
Jeol 1010 TEM with side mount Gatan 1kx1k CCD
Jeol JSM 6700F Field Emission SEM
Specimen preparation
Leica High Pressure Freezer EMPACT2 and RTS
2 Leica AFS freeze substitution and low temperature embedding devices (1 AFS1, 1 AFS2 with FSP)
2 Leica Ultracut UCT room temperature
Reichert Ultracut S room temperature
2 Leica Ultracut UCT, FCS cryo
Knife maker
Baltec Med020 dual gun sputter coater (carbon and carbon/platinum) and rotary shadower
2 Critical Point Drier 3000
Polaron sputter coater
Gatan ion beam coater
Emitech freeze substitution K775X
FEI Vitrobot Mark IV
Software expertise
Gatan Digital Micrograph
Inspect 3D
Amira
Imaris
Drishti

Super-resolution Microscopy
API OMX V3 structured illumination super-resolution microscope

London, CRUK-LRI

Francois Lassailly
+44 (0)207 269 3426

In vivo Imaging Facility
London Research Institute
Cancer Research UK
44 Lincoln's Inn Fields
London
WC2A 3PX

Access

Primarily for LRI users but could be discussed for external users

Expertise
Non-invasive in vivo imaging.
2D, 3D, 4D imaging.
Longitudinal studies for orthotopic models in oncology and hematology, normal development, stem cells, microenvironment, vascular and bone biology.
Assessment of validation of probes and contrasting procedures.
Image processing and analysis

Whole body Bioluminescence (including diffuse optical tomography and spectral unmixing)
Caliper Life Sciences IVIS-Spectrum
Whole body Fluorescence (including epifluorescence, transillumination, tomography and spectral unmixing from UV to IR))
Caliper Life Sciences IVIS-Spectrum
Micro-CT (including physiological monitoring for respiratory or cardiac gating, 4D)
Skyscan 1176 in vivo scanner
Intravital microscopy of live tissues (combining multiphoton and confocal, equiped with multiparametric physiological monitoring)
Zeiss LSM710-NLO

London, LSHTM

Theresa Ward
+44 (0)207 612 7869

Liz McCarthy and Rachel Gregory (LM)
+44 (0)207 927 2063

Maria McCrossan (EM)
+44 (0)207 927 2373

Liz King (Flow Cytometry)
+44 (0)207 927 2373

Woolfson Cell Biology Facility
Immunology Unit
London School of Hygiene & Tropical Medicine
Keppel Street
London
WC1E 7HT

Access

Internal (90% on all instruments)
External academic (10%)
DIY or Full service work

Expertise
Imaging pathogen-host interactions, at cellular level, and in whole model organisms. Includes live imaging under Cat 2 and full aerosol Cat3 containment.

Future requirements
Multiphoton for cell and in vivo imaging under containment

Confocal microscopy
Zeiss LSM 510 invert with live cell chamber and Eppendorf microinjeciton
Fluorescence imaging
Zeiss Axioplan 2 upright with CCD, epifluorescence and histology
Keyence BZ-8000 automated fluorescence microscope under Cat3 containment
Volocity software
Laser capture/microdissection
Arcturus Pixcell IIe
In vivo imaging under Cat3 containment
IVIS Spectrum (Xenogen)
Fluorescence molecular tomography (FMT) system
Electron microscopy
Jeol JEM-1200 EXII TEM
Jeol JSM-25S III SEM
Flow cytometry
2x Becton Dickinson FACSCalibur (one under Cat3 containment)
Becton Dickinson FACSVantage SE cell sorter

London, National Physical Laboratory

Alex Knight (Biotechnolgy Group)
+44 (0)208 9436308

Daniel Metcalf (Biotechnology Group)
+44 (0)208 9436344

National Physical Laboratory
Analytical Sciences Division
Hampton Road
Teddington
TW11 0LW

Access

Internal
External commercial contract research
External academic collaborations welcome

Expertise
Super-resolution microscopy, Single molecule fluorescence, Confocal microscopy, Adaptive optics, Optical coherence tomography, Instrumentation, Metrology/standards

Super-resolution Microscopy:
Direct stochastic optical reconstruction microscope (self-build)
Single molecule fluorescence microscope (self-build)
Structured illumination microscope (self-build, ready early 2012)
Photoactivated localisation microscope (self-build, ready early 2012)
Other imaging techniques available at NPL include:
Confocal fluorescence microscope (Olympus FV1000), Scanning ion conductance microscopy, atomic force microscopy, raman microscopy, secondary ion mass spectrometry, optical coherence tomography, X-ray photoelectron spectroscopy, matrix assisted laser desorption ionisation mass spectrometry, scanning electron microscopy, terahertz imaging, ultrasound

London, NIMR

Yan Gu
+44 (0)208 816 2621

Confocal Imaging and Analysis Lab
National Institute for Medical Research
The Ridgeway
Mill Hill
London
NW7 1AA

Access

Core funded facility by MRC, and shared freely by users within NIMR
External users are charged to use the facility

Expertise
Live cell imaging, CARS, small animal MRI.
Justin Molloy's course in light microscopy.
User support, collaboration on biological imaging projects. Improving microscope performance, and development and implementation of advanced imaging techniques such as super resolution imaging, new probes, live cell/tissue imaging, correlative microscopy.

Future requirements
Imaging in tissues and whole animals, CLEM

Confocal microscopy
Leica TCS SP1 upright
Leica TCS SP2 upright
Leica TCS SP2 AOBS inverted
Leica TCS SP5 upright
Leica TCS SP5 Multiphoton upright
PerkinElmer UltraView LCI Spinning Disk
Fluorescence imaging
2 X DeltaVision RT Live cell imaging microscope
Inverted DeltaVision DV2 widefield fluorescence microscope

London, QMUL

Ann Wheeler
+44 (0)207 882 2406

Andy Bushby (EM)

Gary Warnes (Flow cytometry)

Cleo Bishop (High Content Screening)

Blizard institute Advanced Light Microscopy facility
Blizard Institute
QMUL
4 Newark Street
London
E1 2AT

Access

Priority given to Blizard Institute users followed by QMUL then external
External commercial and academic work as required
DIY or full service available

Expertise
Core Facility.
Super-resolution imaging
Image Processing
FRET/FRAP
Tile-scanning of large areas

Confocal microscopy
Zeiss LSM 710 META upright
Zeiss LSM 510 invert with live cell imaging chamber
Andor Spinning Disk system with FRAP and EMCCD camera
Fluorescence imaging
Zeiss Axiovision 200M inverted with Prior x,y,z stage
Leica DM5000 with additional histology camera
Leica DM4000
MBF Stereology system

Zeiss AV200 PALM lasercapture/microdissection
Leica MZ7s dissection microscope with colour camera
Software
Metamorph, Imaris, ImageJ + Fiji, Matlab, Cell Profiler

Electron microscopy
Carried out by the Nanovision centre
Flow cytometry
Carried out by the Flow cytometry facility
High Content Screening
InCell 1000 imaging system

London, UCL

Jeremy Hebden
+44 (0)207 679 0280

Biomedical Optics Research Laboratory
Department of Medical Physics & Bioengineering
University College London
Malet Place Engineering Building
London
WC1E 6BT

Access

Highly variable, depending on demand

Expertise
Functional optical tomography of the neonatal brain. Real-time optical imaging of seizure in infants, including associated haemodynamic activity in the deep brain.

In vivo imaging systems
32-channel time-resolved optical tomography system
32-source, 16-detector optical topography system
Lasers
IMRA dual-wavelength fibre laser
Fianium supercontinuum laser
Ti:S picosecond las

London, UCL

Andrew Vaughan (LM)
+44 (0)207 679 7904

Jemima Burden (EM)
+44 (0)207 679 3650

MRC Laboratory for Molecular Cell Biology Unit
University College
Gower Street
London
WC1E 6BT

Access

Mostly internal
Small number of external users; about one or two a year

Expertise
Core facility.
Collaboration and development of techniques, image processing and analysis

EM facility - Preparation cell biological material for TEM and SEM. Cryo preparation techniques for biological samples (HPF and FS, and Tokuyasu)

CLEM, 2D and 3D

Future requirements
Tools for image analysis and data processing

Light Microscopy Facility
Confocal microscopy
Leica TCS SP5 with DMI6000 Inverted with motorised stage and Pe-Con stage-top incubator
2 Leica TCS SPE with DM2500 Uprights
Bio-Rad Radiance 2100 with Nikon E800 Upright
Perkin Elmer Ultraview Vox spinning disc with Nikon Ti Inverted, Solent Scientific environmental chamber, Prior stage and piezo focus
Time-lapse microscopy
Zeiss Axiovert 135TV with LEP focus, stage, filters and shutters, environmental chamber for time-lapse imaging, Volocity software
Zeiss Axiovert 200M with LEP stage and shutter, environmental chamber for time-lapse imaging, Volocity software
Fluorescence microscopy
Four Zeiss upright and one Leica inverted microscope/camera systems using Improvision Openlab capture software
Software Packages
Two Volocity 5 systems with all analysis packages. One 32-bit Mac and one 64-bit PC. One Metamorph 7.5 analysis station. Support for Leica LAS AF and Image J

Electron Microscopy Facility
Electron microscopy
FEI Tecnai G2 Sprit TEM 120kV, with tomography stage and holder, side mount SIS Morada 11mpx digital camera
FEI-Philips CM10 TEM with side mount Gatan 1kx1k digital camera
Specimen preparation
Leica High Pressure Freezer EMPACT
2 Leica AFS freeze substitution and low temperature embedding devices (1 AFS1, 1 AFS2 with FSP)
Leica Ultracut E room temperature
Leica Ultracut UCT room temperature
Leica Ultracut UCT, FCS cryo
Leica Knife maker
Baltec Med020 dual gun sputter coater (carbon and carbon/platinum), rotary shadower, freeze drier, glow discharger
Biorad Critical Point Drier 3000
Software
SIS iTEM
Inspect 3D
Amira

London, UCL

Chris Thrasivoulou
+44 (0) 207 670 0904

Tim Robson

Danial Ciantar

Jane Pendjiky

Centre for Cell & Molecular Dynamics
UCL Faculty of Life Science
Imaging Centre (Biosciences)
5th Floor
Rockefeller Building
21 University Street
London
WC1E 6JJ

Access

All users welcome (Internal and External)
Flat rate for all confocals £20 per hour for all users
Overnight rate £10 per hour

Expertise
The unit has four experienced members of dedicated staff who are on hand at all times. Full training of all users at no extra cost. Free consultancy service available for help with experimental design and imaging, trouble-shooting requirements.

Future requirements
New microscopes will be added in the future to cater for research needs. This will include spinning disk confocals, resonant scanning confocals and Multiphoton-OPO systems for Second and Third Harmonic Generation microscopy that this unit is developing.

Confocal microscopy
Leica SP2 UV upright multiphoton, FLIM live-cell imaging
Leica SP2 fixed-stage upright, multiphoton, live-cell imaging
Leica SP2 upright, single photon system
Leica SP1 upright UV/ single photon system
Leica SP1 upright single photon system
Leica SP1 fixed-stage upright, single photon system
Leica SPE (x2) upright, single photon system, live-cell imaging
Leica SPE inverted, single photon system, live-cell imaging
Olympus Flowview 1000, Sim-Scan, TIRF single photon system, live-cell imaging, ZDC
Zeiss 510 upright/invert multiphoton, live-cell imaging
Zeiss 510 META UV-VIS, inverted single photon system
Zeiss 510 META inverted VIS, inverted single photon system
Zeiss 700 inverted, single photon system
Fluorescence Wide-Field Microscopy
Zeiss AxioPlan 400 inverted live cell imaging with Orca camera
ZeissAxioPlan2 upright, laser ablation, Orca camera
Olympus IX81 inverted, live cell imaging with ZDC

London, UCL, Centre for Advanced Biomedical Imaging

Mark Lythgoe
+44 (0)20 7679 6164

Centre for Advanced Biomedical Imaging
Paul O'Gorman Building
University College London
72, Huntley Street
London
WC1E 6DD

Access

Internal and External commercial & external academic available
DIY or Full service work

Expertise
Core Facility; In vivo Preclinical Imaging; In vivo Tumour, Cardiac and Neuro-Imaging; Optogenetics; Magnetic nanoparticles; Probe and MRI contracts agent development; Multimodal Image analysis; Brain atlas generation; In Vivo Experimental models (eg stroke and myocardial infarction)

In vivo Preclinical Imaging:
9.4T Magnetic Resonance Imaging: Agilent Technologies
Photoacoustic Imaging: based upon the use of Fabry Perot polymer film sensing interferometers
Ultrasound: A Vivid 7 Dimension (GE Healthcare)
SPECT/CT: Bioscan system
Bioluminescence and fluorescence imaging system: Biospace Lab PhotonIMAGER™
CT-guided in vivo irradiation: Xstrahl system

London, UCL, ICH

Bertrand Vernay
+44 (0)2079052224/2715

Confocal Microscopy Core Facility
UCL Institute of Child Health
30 Guildford Street
London
WC1N 1EH

Access

All users welcome (Internal and External) academic and commercial.
Flat rate for all confocals £20 per hour for UCL users, overnight rate £10 per hour (please contact facility manager for external rates).
DIY or full time support if required.

Expertise
Image acquisition of fixed and live samples with OpenLab, Volocity, Leica and Zeiss confocal softwares.
Image processing and quantitative analysis with Volocity, ImageJ and CellProfiler.
Help for manuscript preparation: Photoshop, Illustrator, InDesign

Confocal microscopy
Leica SP2 UV-VIS upright, multiphoton excitation, live-cell imaging (inverted stand available), FRAP, FRET
Zeiss 710 UV-VIS, inverted single photon system, live-cell imaging, FRAP, FRET, spectral imaging
Widefield microscopy
Zeiss Axiovert 135 Fluorescence widefield microscope, live cell imaging (OpenLab, Volocity acquisition software)

London, UCL, WIBR

Tomas Adejumo
Flow Cytometry and Confocal Microscopy Manager
Tel1: +44 (0)207679 6719
Tel2: +44 (0)207679 6895

George Chennell
Applications Specialist - Laser Micro-Dissection / Confocal Microscopy/ Live Cell Imaging/ Seahorse Bioanalyser
Tel: +44 (0)207679 6757

Scientific Support Services
UCL Cancer Institute and Wolfson Institute for Biomedical Research
Gower Street
UCL London
WC1E 6BT

Access

Internal (75% on all instruments)
External commercial and external academic (25% on all instruments)
DIY or full service work

Expertise
Core facility; software analyses; High resolution imaging

Confocal microscopy
Leica TCS SP
PerkinElmer UltraView Volocity Acquisition Spinning Disk
Fluorescence microscopy
Zeiss CellObserver Live Cell Imaging Microscope
Zeiss AxioImager A1 fluorescent microscope
PALM laser capture/microdissection
Flow cytometry
Beckman Coulter CyAn flow cytometer
Beckman Coulter MoFlo high-speed sterile cell sorter
Software
Volocity Visualisation and Quantification

Manchester

Peter March
+44 (0)161 275 1571

University of Manchester Bioimaging Facility
Faculty of Life Sciences
Michael Smith Building
Oxford Road
Manchester
M13 9PT

Access

Internal (95% on all instruments)
External commercial & external academic (5% on all)
Mainly DIY or piloted

Expertise
Expertise in live cell imaging and particle tracking.
High resolution imaging.

Future requirements
Faster live imaging and in vivo animal imaging

Confocal microscopy
Leica SP5 Tandem Head (inverted)
Leica SP5 MP (upright)
Leica SP2 MP (inverted)
Nikon C1 (upright)
Nikon C1/TIRF (inverted)
Nikon Swept Field Confocal (inverted)
Widefield microscopy
API Delta Visions
MetaMorph based live cell system with FRAP
Image Pro live cell imaging systems
BD Pathway 855 high throughput system
PALM laser microdissection

Manchester, Paterson

Steve Bagley
+44 (0)161 446 8631

Advanced Imaging Facility (CR UK)
Paterson Institute for Cancer Research
University of Manchester
Wilmslow Road
Manchester
M20 9BX

Access

CR UK Internal 80% on all instruments
CR UK external access 20%

Expertise
Core Facility.
High mag/resolution low light time lapse, FRAP, FRET, photoactivation

Confocal microscopy
Roper CSU22 spinning disk (invert)
Fluorescence imaging
EM CCD imaging (invert)
Live cell imaging systems/chambers
Deltavision Core (invert)
Roper FRAP on the Fly system (invert)
CRI Nuance
Histology imaging
Mirax Scan (300 slide Histology Scanner)

Norwich, John Innes

Kim Findlay
+44 (0)1603 450668

Grant Calder
+44 (0)1603 450298

Peter Shaw
+44 (0)1603 450330

BioImaging Facility
Department of Cell and Developmental Biology
John Innes Centre
Norwich Research Park
Norwich
NR4 7UH

Access

Internal on all instruments
External commercial & external academic (on application)
DIY or Full service work

Expertise
Expertise in plant cell biology, in vivo imaging.
High resolution cryo-SEM, GFP technology, General TEM sample prep and imaging

Future requirements
Access to super resolution, image analysis especially tools for shape recognition in organisms

Confocal microscopy
Zeiss LSM 510 META upright
Leica SP1 upright
Leica SP2 invert
Visitech QLC100 Spinning Disk Upright
Fluorescence imaging
Leica DFM6000
CCD imaging
Zeiss PALM laser capture/micro dissection
Live cell imaging chambers
Electron microscopy
FEI Tecnai 20 TEM
FEI XL30 FEG SEM
Zeiss Supra 55VP FEG SEM
GATAN Cryosystems on both SEMs Deben Coolstage for Zeiss SEM

Norwich, UEA

Paul Thomas
+44 (0)1603 592196

Alba Warn
+44 (0)1603 591776

Henry Wellcome Laboratory for Cell Imaging
School of Biological Sciences
University of East Anglia
Norwich
NR4 7TJ

Access

Full access to external users, though internal users get priority.
DIY or Full service work

Expertise
Core Facility.
Live-cell imaging (ion measurements and cell tracking); Image analysis (Intensity and ratiometric analysis, cell tracking, deconvolution, co-localization).
Offers a 2-day course in live-cell imaging.

Confocal microscopy
Zeiss LSM 510 META invert/upright
Leica TCS SP2 invert
LaVision Biotec TriM Scope II with TCSPC
FLIM capability (invert/upright)
Fluorescence imaging
Zeiss Axiovert 200M with environmental chamber for variety of samples
Zeiss Axiovert 200M with environmental chamber for 6- and 24-well plates
Zeiss Axioplan 2ie for fixed specimens
Zeiss SV11 stereomicroscope

Nottingham

Tim Self
+44 (0)115 8230090

Institute of Cell Signalling
School of Biomedical Sciences
University of Nottingham
Queen's Medical Centre
Nottingham
NG7 2UH

Access

Training provided
60% ICS use
Outside collaboration possible

Expertise
Live cell pharmacology, novel ligands.
FCS

Future requirements
Analysis algorithms for HCS approaches

Confocal microscopy
Zeiss LSM710 META invert with live cell perfusion system
Zeiss LSM510uv META invert with live cell perfusion system
Zeiss NLO Confocor3 META FLIM invert
Zeiss Exciter invert live cell + TIRF
Zeiss Confocor2 invert
High Content Screening
Molecular Devices Ultra confocal plate reader + robot
Molecular Devices Micro widefield plate reader + environ

The Open University

Heather Davies
+44 (0)1908 653392

The Open University
Electron Microscopy Suite
Walton Hall
Milton Keynes
MK7 6AA

Access

Internal academic (university wide)
External commercial and academic
Can be used as DIY or with technical help

Electron microscopy
JEM 2100 - a 200kV TEM from JEOL - high resolution EM/tomography, configured with a high tilt polepiece and specimen holder for tomography and has other analytical capabilities
Zeiss Supra 55V - Analytical FEGSEM with variable pressure mode, enabling imaging of vacuum-sensitive and/or non-conductive samples without the need to apply conductive coating
JEM 1400 - a 120kV TEM from JEOL
JEM 1010 - a 100kV TEM from JEOL
Extensive preparation facilities

Oxford

Ian Dobbie
+44 (0)1865 613323

Department of Biochemistry,
University of Oxford,
South Parks Road,
Oxford
OX1 3QU

Access

All instruments are available to internal users
Currently no policy for external users

Confocal microscopy
PerkinElmer Spinning disk
Fluorescence imaging
DeltaVision Core with FRAP module
Olympus IX70 with micro-injection
Other
OMX Super-resolution microscope

Oxford

c/o Chris Hawes

School of Life Sciences
Oxford Brookes University
Gipsy Lane
Oxford
OX3 0BP

Access

Unit services the University
External users welcome
Training available
Oxford Microscopy Consultancy offers a full microscopy service (microscopy@brookes.ac.uk)

Expertise
Live cell imaging, electron tomography, cryo-preparation

Future requirements
Spinning disc confocal, TIRF

Confocal microscopy
Zeiss LSM 510 META upright
Zeiss LSM 510 invert
Fluorescence
Zeiss Axioplan +CCD
Electron Microscopy
Hitach H-7650 120 kV tomographic TEM
Hitachi S-3400N variable pressure SEM
Preparative equipment
Baltec HPM 010 high pressure freezer
freeze-substitution
RMC PowerTome PC with CR-X cryo attachment

Flow cytometry
Becton Dickinson FACSCalibur cytometer

Oxford, MRC Harwell

Jeremy Sanderson
+44 (0)1235 84 1051 (office)
+44 (0)1235 84 1181/1037 (labs)

Mammalian Genetics Unit
MRC Harwell
Harwell Science and Innovation Campus
Didcot
Oxfordshire
OX11 0RD

Access

Internal on all instruments
External contracted work by appointment

Confocal microscopy
Zeiss 710 Laser Scanning Confocal Microscope
Leica TCS SP5 Laser Scanning Confocal Microscope
Multi-photon microscopy
Zeiss 710 NLO on upright Axio-examiner Z.1 + BiG GaAsP detectors (on LSCM above)
Fluorescence microscopy
Olympus IX-81 inverted + XM10 camera
Olympus BX-41 upright + CFW1312M camera
Zeiss Axiophot2 upright + AxioCamHRc camera
Leica DM2500 + Hamamatsu 9300 fast-frame camera
Widefield microsopy
Olympus BX-51 upright + DP25 digital colour camera
Electron microscopy
JEOL JSM-6010LV SEM
Image analysis
Perkin-Elmer Volocity 5

Pirbright, Institute for Animal Health

Pippa Hawes
+44 (0)1483 231103/231026

Bioimaging
Institute for Animal Health
Ash Road
Pirbright
Surrey
GU24 0ND

Access

Internal (mainly)
External academic or commercial collaboration possible

Expertise
Core Facility, containment microscopy
Cell biology and virology
Confocal and transmission electron microscopy
Live cell confocal microscopy at SAP04 containment

Future requirements
TIRF, multi-photon, image analysis, full data management system

Confocal microscopy
Leica SP5 (upright and invert)
Leica SP2 (upright)
Leica SP2 (invert, for live cell imaging at SAP04 containment)
Vibrating microtome, cryostats, LMD's, fluorescent microscope, camera systems
Electron microscopy
FEI Tecnai 12 120kV TEM, with Tietz F214 camera
Cyro-TEM and dual axis tomography holders
Leica CPC plunge freezer
Leica HPM100 HPF
Leica AFS, AFS2
Leica UC6 ultramicrotome
Leica UC7 cryo-ultramicrotome
Software
Inspect 3D, IMOD, Amira

Rothamsted

Allison van de Meene
+44 (0)1582 763 133

Rothamsted Bioimaging
PPM Department
Rothamsted Research
Harpenden
AL5 2JQ

Access

Internal and external, academic research groups and commercial
Independent or full service work

Expertise
Core Facility.
Plant cell biology.
Live cell microscopy, sample preparation for sectioning, sectioning, cryoSEM, EDS, high resolution TEM, electron tomography and image analysis.

Future requirements
In vivo imaging and image analysis tools

Confocal microscopy
Leica TCS SP1 inverted
Widefield & fluorescence microscopy
Zeiss upright and inverted microscopes with CCD imaging both in colour and monochrome
Timelapse capabilities
PALM laser capture/microdissection
Leica M205 FA automated stereomicroscope
Electron microscopy
Jeol 6360 LVSEM with Gatan Alto 2100 cryosystem, Deben coolstage and Oxford EDS
High resolution Jeol 6700F FEG-SEM with Gatan Alto 2500 cryosystem and Oxford EDS
Jeol 2011F TEM with Gatan dual-axis holder for tomography, Gatan cryoholder and Oxford EDS
Image Analysis
Amira, MetaMorph, ImageJ

Sheffield

Colin Gray
+44 (0)114 2261234

Light Microscopy Core Facility
Faculty of Medicine, Dentistry and Health
The University of Sheffield
Beech Hill Road
Sheffield
S10 2RX

Access

Internal on all instruments
External commercial & academic on application
DIY or full support service work

Expertise
Core facility, multiphoton, time lapse imaging

Confocal microscopy
1 Inverted multiphoton Zeiss LSM510 META with a Coherent Chameleon MP laser and environmental control
1 Upright multiphoton Zeiss LSM510 META with a Coherent Chameleon MP laser with dipping objectives and environmental control
Becker and Hickl FLIM
Live cell imaging
Leica AF 6000LX with full environmental control
Fluorecence microscopy
Leica DMI 4000B
Image Processing
Central server with remote access and 2 offline workstations

Sheffield

Darren Robinson
+44 (0)114 2224682

Light Microscopy Facility
University of Sheffield
Alfred Denny Building
Western Bank
Sheffield
S10 2TN

Access

Internal (90% on all instruments)
External commercial & external academic (10% on all)

Expertise
Core facility

Confocal microscopy
Zeiss LSM 510 invert
Olympus FV1000 Upright with SIM scanner
Leica SP1
PerkinElmer UltraView Spinning Disk
Nikon A1 invert with TIRF
Fluorescence imaging
EM CCD live cell imaging
Deltavision RT with quantifiable laser module
High content
Molecular Devices MetaExpress Micro

Sheffield

Nicola Green
+44 (0)114 2223932

Kroto Research Institute Confocal and Two-Photon Imaging Facility
Kroto Research Institute
North Campus
University of Sheffield
Broad Lane
Sheffield
S3 7HQ

Access

Internal
External commercial & external academic
DIY or with Experimental Officer support

Expertise
Core facility; Two-photon imaging of biological tissue

Confocal microscopy
Zeiss LSM 510 META NLO invert with environmental chamber
Zeiss LSM 510 META NLO upright (with in house modifications for time resolved emission microscopy)
Fluorescence imaging
Zeiss epifluorescent inverted microscope with environmental chamber

Southampton

Anton Page
+44 (0)23 8079 4815

Biomedical Imaging Unit
Level B South Block MP12
General Hospital
Southampton
SO16 6YD

Access

All users welcome (Internal and External)
Diagnostic work 50% (internal & external)
Academic 50% (mostly internal).
Independent or full service work.
Commercial service available.
Free instrument training of all users.
Consultancy service to help with experimental design, imaging and analysis

Expertise
Joint University & NHS Trust core facility.
The Unit has 5 experienced full time staff supporting research and diagnostic work.
Staff support on hand at all times.

Confocal microscopy, live cell imaging, TEM, SEM, x-ray microanalysis, diagnostic electron microscopy (renal, nerve, muscle etc), national centre for the diagnosis of primary ciliary dyskinesia.
Art-science collaborations
Artist in residence
Training:
One week TEM course (IBMS accredited)
One week advanced LM course (IBMS accredited)
Trainee biomedical scientists specialising in electron microscopy (graduate day-release)

Future requirements
3D light and electron microscopy.
Correlative light & electron microscopy.
Low power confocal imaging.
3D rendering and visualisation software.

Confocal microscopy
Leica SP2
Leica SP5 (Inverted, with live cell imaging & resonant scanner)
Electron microscopy
FEI Tecnai 12 TEM (with XRM)
Hitachi H7000 TEM
FEI Quanta 200 SEM (with XRM, variable pressure & environmental modes)
Full TEM & SEM preparation facilities including cryosectioning (all independent or full service)
Fluorescence imaging
Olympus IX 81 (with live cell imaging)
Zeiss Axioskop 2
Leica DM IRBE (with live cell imaging)
Leica DM RBE
Light microscopy
Olympus DotSlide with remote access
High speed video microscopy (with live cell imaging)
Image analysis
SIS iTEM, SIS Cell^P, Zeiss KS software

Southampton

Hans Schuppe
+44 (0)23 8059 4235

Imaging and Microscopy Centre
School of Biological Sciences
Life Sciences Building 85
University of Southampton
Highfield Campus
Southampton
SO17 1BJ

Access

Open to all users (academic, non-academic)
Internal (85% on all instruments)
External commercial, external academic & external public (15% on all instruments)
DIY or full service work

Expertise
Modern Core Facility with containment level 1 and 2 labs.
Live cell imaging, Multiphoton microscopy, Fluorescence correlation spectroscopy (FCS), Electrophysiology.

Future requirements
Super-resolution fluorescence microscopy.

Confocal microscopy
Leica TCS SP2 FCS multiphoton upright with environmental chamber and stage-top incubator
Zeiss LSM 510 META upright with fixed stage and UV laser
Fluorescence imaging
Zeiss Axiophot 2 upright
Zeiss Axioskop 2 upright with fixed stage
Zeiss Axiovert 200 invert
Leica DM IRB invert with Leitz micromanipulators
Live cell imaging chambers
Electrophysiology
Axoclamp-2A amplifier
Axopatch 200B amplifier
Preparation room
Wolf Lab. Galaxy S Incubator
Microbiological Safety Cabinet Class II
Leica MZ 16F stereomicroscope

Strathclyde

Gail McConnell
+44 (0)141 548 3895

Dave Blatchford
+ 44 (0)141 548 4694

Centre for Biophotonics
Institute for Pharmacy and Biomedical
Sciences
University of Strathclyde
27 Taylor Street
Glasgow
G4 0NR

Access

Internal
External commercial & external academic (20% on all)
DIY only (following in-house training)

Expertise
Can offer CARS and expertise in optical trapping.
Core Facility.
Optical instrumention development and high-resolution optical imaging.

Future requirements
New tools for illumination and light sources

Confocal microscopy
Bio-Rad 1024/Nikon Eclipse E600FN upright microsocpe
Bio-Rad 1024/Nikon Eclispe TE300 inverted microscope
Leica TCS SP5 with DM6000B upright microscope
Multi-photon/confocal microscope
Bio-Rad Radiance & Coherent Chameleon laser with Nikon Eclipse TE300 inverted
Fluorescence imaging
Upright Nikon E600F epi-fluorescence microsocpe
Nikon TE300 inverted microscope with Solent Scientific environmental chamber, Sutter Scientific Lambda 12 controller
Lasers
Coherent Mira-F (2x: 150 fs, 700-920nm)
Coherent Mira-D (150ps or 2ps, 700-920nm)
Fianium 2W Yb:YAG laser (300fs, 1064nm)
IPG Yb:YAG laser (10W, cw)
Del Mar Cr:Forsterite laser with frequency doubling option (300mW & 120 fs @ 1240-1280nm or 100mW & 120 fs @ 620-640nm or 60 mW & 1.5 ps @ 620-640 nm)
OPO I (~150 fs or ~2ps, 920-1180 nm)
OPO II (~300 fs, 1380-1600nm)
Systems that *may* become part of facility in due course - availability currently upon request:
CARS microscope based on Olympus FV300 & modified Olympus IX inverted platform
Optical trapping & fluorescence system - suitable for live cell manipulation
Olympus FV1000 with dual-scanning system/IX inverted microscope

York

Peter O'Toole
+44 (0)1904 328722

Jo Marrison
+44 (0)1904 328720

Imaging and Cytometry Laboratory
Technology Facility Department of Biology
University of York
York
YO10 5DD

Access

Internal (80% on all instruments)
External commercial & external academic (20% on all)
DIY or Full service work

Expertise
Core Facility.
Probe development and high resolution imaging.
Offers a range of microscopy courses.

Confocal microscopy
Zeiss LSM 780 META multiphoton invert
Zeiss LSM 710 META invert
Zeiss LSM 510 META invert
Zeiss LSM 510 META upright
Andor Revolution XD Spinning Disk
Fluorescence imaging
EM CCD imaging
PALM laser capture/microdissection
Live cell imaging chambers
Electron microscopy
FEI Tecnai 12 TEM
Jeol 6490 SEM with cryosystem
High resolution Jeol 7500F FEG SEM
GATAN CL and Cryo
Clairscope ASEM

Flow cytometry
2 Beckman Coulter CyAn flow cytometers
Beckman Coulter Astrios high-speed sterile cell sorter
Apogee flow system A40