Genever Lab Group

SOPs (Standard Operating Procedures)

These SOPs are the current definitive versions. To edit SOPs, go to the relevant folder within LAB INFORMATION on the R:/ drive where you will find a link to each SOP. Make sure you save new or edited SOPs in the Website folder.

 

1.

Trizol Extraction of RNA

2.

DNAse Treatment of Total RNA

3. cDNA Synthesis
4. Stripping Western Membranes
5. PCR
6. Western Blotting
7. Aseptic Technique
8. Isolation of human MSCs from femoral heads
9. Flow Cytometry / Flow Cytometry for Fixed Samples
10. Creation of bone fracture mouse model
11. Cfu-f Assay
12. Extraction of cells from umbilical cord blood
13. Extraction of mononuclear cells from human peripheral blood
14. Mycoplasma Testing
15. Incubator Cleaning
16. Class II Cabinet Cleaning
17. Extraction of DNA from MSCs
18. Dynamic 3D cultures of MSCs
19. Subculture of Embryonal carcinoma cells
20. Mouse embryonic fibroblasts
21. Subculture of hES cells
22. Flow cytometric analysis of cell cycle
23. Preparation of 2D vs 3D MSC-conditioned medium
24. MSC differentiation
25. Alizarin red staining of monolayers
26.

ALP von Kossa staining

27.

Western Blot (UPDATED)

28. PNPP Assay
29. Oil Red O staining