Research Facilities

Characterisation of Proteins

YSBL maintains its own wet laboratory facilities with the expertise and equipment for gene cloning, PCR, sequencing and protein overexpression in a variety of systems from small to large (fermenter) scales in both bacterial (E. coli) and baculovirus driven insect cell systems. For proterin purification, there are several low and high pressure chromatography systems and a large number of different chromatographic matrices for optimal protein purification. The laboratory has access to a range of biophysical characterisation techniques such as light scattering, a range of mass spectrometers and analytical ultracentrifugation through the Bioscience Technology Facility. This includes access to methods for biomolecular analysis of proteins and their complexes such as UV, fluorescence and time-resolved spectroscopy, microcalorimetry and surface plasmon resonance.

The primary goal of these facilities is to provide a regular supply of pure, homogenous and functional protein for crystallisation.

Chemical Biology Facility

Please see Chemical Biology Facility.

Crystallisation

YSBL maintains a dedicated crystallisation suite, consisting of nanolitre-drop dispensing Mosquito robots, an experimental crystal imaging system and a large, temperature controlled cystallisation room. In addition, there are series of incubators for crystallisation at different temperatures. Staff at YSBL are pioneers in new methods of crystallisation and many of the screens marketed worldwide were invented here.

X-Ray Diffraction

Please see the X-Ray Facilities.

Biomolecular NMR

The Centre for Magnetic Resonance, located in the Department of Chemistry, includes two instruments equipped for NMR structural studies of protein and of protein:ligand interactions in solution. The Bruker AVANCE 500MHz and Bruker AVANCE II 700MHz spectrometers are both equipped with shielded magnets and triple (1H, 15N, 13C) resonance inverse, broadband inverse and broadband probes.