Contact Alison for opportunities in the group
I am interested in how oxidation state changes at transition metal centres in proteins activate chemical reactions which are essential for life. Understanding such processes is important across a wide range of fields: a fundamental understanding of how bacteria derive the energy for life is essential if we are to develop new antibiotics to halt the rise in drug-resistant pathogens; learning how H2 and other small molecule activation occurs efficiently and rapidly at non-precious metal centres teaches us how to design better industrial catalysts; and finding out how novel proteins function in humans reveals how we work as big reaction vessels of living chemistry.
In the laboratory the main technique used to interrogate these redox reactions is film electrochemistry. This method permits precise control of the reduction-potential of the protein so that measurement of current provides a quantitative measure of the rate of redox reactions. Thermodynamic and kinetic reaction parameters are determined quantitatively, and enzymatic reaction mechanisms can be probed through analysis of substrate catalysis and inhibitor sensitivity. To complement the functional insight that electrochemistry provides, structural information is determined with molecular biology, protein crystallography, electron paramagnetic resonance and infra-red spectroscopy.
We also apply our electrochemical toolkit to probe synthetic CO2 chemical reactions, collaborating with Prof Mike North to develop new capture and utilisation methodologies and with Prof Peter O'Brien and Dr Victor Chechik to harness Kolbe chemistry for late-stage functionalisation.
We are working with the world-class Australian electrochemist Professor Alan Bond (https://www.monash.edu/science/schools/chemistry/our-people/staff/bond) to develop his technique, large amplitude Fourier transform alternating current voltammetry, as a powerful tool for studying redox active proteins and enzymes via film electrochemistry. We refer to this technique as “PF-FTacV” and our joint review paper explains why this technique yields such highly sensitive and powerful measurements: http://pubs.rsc.org/en/content/articlelanding/2017/cc/c7cc03870d#!divAbstract.
In living cells, oxidation and reduction reactions are coupled together across a membrane to generate electron gradients which ultimately provide the energy for ATP synthesis and life. Many disease causing bacteria are able to use H2 as a fuel in this process, with H2 uptake (H2 2H+ + 2e-) having proved very important in enabling Salmonella, Mycobacterium tuberculosis and Helicobacter pylori (a stomach cancer-causing agent) to survive. The function and structure of these enzymes and the biosynthetic pathways of assembly will be probed to understand how this chemistry works and how it can be shutdown in order to try and develop new antibiotic drugs. Research in this area is in collaboration with Professor Frank Sargent (University of Dundee) and Professor Greg Cook (University of Otago, New Zealand).
Microbes are fantastically inspirational chemical factories: they produce highly efficient and active catalysts which use common metals and often operate at standard temperature and pressure. Much of the small molecule chemistry which occurs in bacteria is industrially important to humans and studying the enzymes which catalyse this chemistry can teach us how the transition metal centres function. We can then manipulate the DNA of the bacteria in order to force the bacteria to biologically synthesis novel ligand environments to specifically re-tune the chemical specificity. Some of the enzymes being studied are:
Alison Parkin arrived in York in October 2012 from the University of Oxford. Alison is interested in how microbial enzymes can inspire the development of novel biotechnology, and the structure-function relationship which controls such chemistry. Her group develops new techniques as well as exploring new systems, including H2-redox enzymes, Mo-enzymes of unknown function, and Cu-cellulase proteins. Alison also teaches undergraduate Chemistry and Biochemistry which she enjoys. Alison originally hails from Yorkshire and very much enjoys living and working in God's own country!
I'm the technician for the Parkin-Fascione Groups three days a week. I studied Joint Honours Chemistry and Biochemistry at Nottingham University, and then worked in the Biotechnology Industry as a Biochemist for several years. I was lucky enough to be a Process Development Scientist for Bayer AG in San Francisco for two years where I helped to develop processes for the production and characterisation of the recombinant protein Factor VIII, which is used in the treatment of haemophilia. After moving back to the UK I joined YSBL here in York, where I worked on many projects including characterising the structure of the Oestrogen receptor protein. After a brief spell as a technician in teaching labs, I moved to my current role 3 years ago. Outside of work I enjoy photography, reading and looking after my three cats!
I am a PhD student shared with Jason Lynam and Ian Fairlamb. My research involves synthesizing transition metal carbonyls and probing their redox properties for therapeutic applications. I enjoy the wide-ranging, interdisciplinary nature of this work. I came to York in 2014 after an MChem at Wadham College, Oxford, where I studied hydrogenase inhibition using spectroelectrochemistry in the group of Professor Kylie Vincent. You might also find me learning about natural history, supporting the mighty Nuneaton Town FC, or attempting various complex aerobics routines.
I am a PhD student shared with Alison Parkin, Peter O’Brien and Victor Chechik. My research involves the late-stage functionalisation of drug-like scaffolds by electrochemically-generated free radicals. I graduated from the University of Leeds (MChem with an industrial placement year). The placement involved research of an opioid addiction treatment drug and its impurities in order for the drug to be approved.
I am a PhD student working on electrochemical CO2 capture. Capacitance, or charge storage, can be used to concentrate CO2 as a reagent and I have used this for solid phase mineralisation by reaction with oxidised aluminium or steel. I am now working towards using this capacitance for CO2 electrochemical reduction to fuels such as methanol or higher hydrocarbons. In my own time I like to play badminton.
I work on the site selective bio-orthogonal modification of proteins with the aim to covalently crosslink proteins to electrode surfaces in orientations suitable for facile electron transfer, and the subsequent electrochemical assay of these immobilised proteins. My work will allow for the electrochemical assay of redox proteins that have proved difficult to analyse by classic protein film voltammetry, allowing the mechanism of these proteins to be probed and kinetic information to be obtained. Immobilisation of proteins via a covalent bond also increases the lifespan of the immobilised protein film, improving the performance of redox proteins as electrocatalysts.
I am a PhD student with Dr Alison Parkin, Dr Victor Chechik and Prof. Peter O’Brien. My research involves the late-stage functionalisation of agrochemical-like scaffolds using synthetic electrochemistry methods. I studied for my undergraduate at the University of York and did my Masters project with the Materials Group studying “Synthesis and Characterisation of Functionalised Liquid Crystals” under the supervision of Dr Stephen Cowling.
I work on electrochemical capture and mineralisation of CO2 under the day to day supervision of Mark Dowsett. I have been a film star during my time with the Parkin group!
I work on harnessing post-translational modification methods for functionalising enzymes under the day to day supervision of Julia Walton. I have also been a film star while working in the group and their resident photographer.
I work on doing what Nick Yates tells me! My project is aimed at synthesising small molecule protein-electrode linkers.
Parkin Group Jan 2017/18
Parkin and Fascione Groups go to a Sheffield Water Park 2017
Parkin Group 2016
Meal with summer students
Summer BBQ 2015
Alison and Hope visit collaborators in Australia
Christmas Party 2014 - Pizza and liquid nitrogen icecream
Welcoming new lab members
Summer student Aaron falls in love with Betty the glovebox
Lab move summer 2014
End of year BBQ 2014
End of term meal at Il Paradiso
David enjoying his birthday with a caterpillar cake
Hope at work in the glovebox
Parkin group - hard at work
Johnathan in a relaxed lab session.
Parkin Group Leavers 2014
Harriet Chidwick MChem student
Worked on hydrogenase oxygen tolerance and supplying the Parkin lab with laughter and biscuits. Now a PhD student in the Fascione group (York).
Charlotte Neil MChem student
Studied CO releasing molecules and introduced Disney music to the Parkin lab. Now a trainee accountant at Sam Rogoff & CO (London).
Emily Brooke MChem student
Worked on copper lytic polysaccharide monooxygenases and bringing a touch of class to the Parkin group. Now a PhD student in the Armstrong group (Oxford).
Alison Fellgett Biochemistry project student
Worked on whole cell electrolysis for hydrogen production and how not to turn cells pink. Now a research technician (York).
David Lloyd Biochemistry project student
Developed a hydrogenase activity assay and left with the glory of being crowned BMTron champion. Now a research scientist at Sense Biodetection (Oxford).
Parkin Group Leavers 2015
Jonathan Balbach MChem student
Worked on understanding the biosynthetic pathway of Salmonella enterica hydrogenase-5 and naming every piece of equipment in the Parkin / Fascione labs.
Studied copper lytic polysaccharide monooxygenases.
Alec Banner Biochemistry project student
Worked on acetylene hydratase enzymes and adding a bit of muscle to the Parkin lab.
Chileab Redwood-Sawyerr Biochemistry project student
Studied molecular biology of hydrogenases.
Jess Munro Biochemistry project student
Worked on whole cell electrochemistry of Methanococcus maripaludis for hydrogen production.
Christopher Orme MRes
Worked on late-stage functionalisation of agrochemical-type scaffolds by electrochemically-generated free radicals.
Nicola Williams MRes
I completed a BSc in Biochemistry at the University of York (I completed my research project working with the Parkin research group) and I am continuing to study here for an MSc by research. I am enjoying my MSc project very much- I am using protein film electrochemistry to further characterise the catalytic mechanism of bacterial molybdenum enzymes. I also enjoy going to Chemistry Department seminars, learning Japanese (I would love to go to Japan one day) and playing piano and oboe.
Huw Watts MChem
Worked on FTAcV of a molybdenum enzyme.
Worked on Laccase enzyme.
Owen Jarman MChem
Performed protein film Fourier transform voltammetry, to study various redox proteins such as DsbA. Performed electrode modifications to enhance protein film voltammetry.