Professor Gideon J. Grogan

+44 (0)1904 328256
Email: gideon.grogan@york.ac.uk

Structural and Applied Enzymology

Gideon Grogan works on the identification, characterisation and application of new enzymes that have potential applications in biotechnology, including those for the production of chiral pharmaceutical precursors and the bioprocessing of renewable materials.  Recent work has focused on:

  • Oxygenases, including cytochromes P450 (P450s) and flavoprotein monooxygenases;
  • Reductases, including those that act on prochiral ketones (KREDs) and imines (IREDs) and enzymes that catalyse reductive amination reactions (RedAms)
  • Lyases that catalyse the asymmetric hydration of alkenes.
  • Ligases for the formation of amide bonds.

In each case, a multidisciplinary approach is adopted, involving synthetic chemistry, microbiology, molecular biology, and X-ray crystallography, with a view to engineering enzymes using in vitro evolution techniques for altered or improved characteristics.

Recent Selected Publications

  • A Mechanism for Reductive Amination Catalyzed by Fungal Reductive Aminases (RedAms)
    M. Sharma et al, ACS Catal., 2018, 8, 11534-11541
  • The Broad Aryl Acid Specificity of the Amide Bond Synthetase McbA Suggests Potential for the Biocatalytic Synthesis of Amides
    M. Petchey et al, Angew. Chem. Int. Ed., 2018, 57, 11584-11588
  • Biocatalytic conversion of cyclic ketones bearing alpha-quaternary stereocenters to lactones in an enantioselective radical approach to medium-sized carbocycles
    C. Morrill et al, Angew. Chem. Int. Ed., 2018, 57, 3692-3696
  • Synthesis of Chiral Amines Using Redox Biocatalysis
    G. Grogan, Curr. Opin. Chem. Biol., 2018, 43, 15-22
  • Biocatalytic Routes to Enantiomerically Enriched Dibenz[c,e]azepines
    S.P. France et al, Angew. Chem. Int. Ed., 2017, 56, 15589-15593
  • A Reductive Aminase from Aspergillus oryzae
    G. Aleku et al, Nature Chem., 2017, 9, 961-969
  • Structural and functional insights into asymmetric enzymatic dehydration of alkenols
    B. M. Nestl et al, Nature Chem. Biol. 2017, 13, 275-281
  • Snapshots of the Catalytic Cycle of the Industrial Enzyme α-Amino-ε-Caprolactam Racemase (ACLR) Observed Using X-ray Crystallography
    A. Frese et al, ACS Catal., 2017, 7, 1045-1048