Pulling apart proteins and their complexes using force

Friday 4 October 2013, 12.15PM

Speaker(s): Dr David Brockwell, Institute of Molecular and Cellular Biology, University of Leeds.

Colicin E9 is a nuclease antibiotic produced by E.coli to target and kill competing bacteria in times of stress. Immunity protein 9 (Im9) is expressed co-translationally and binds strongly to E9, inactivating the nuclease to protect the producing cell from the cytotoxic effects of E9.  The affinity between E9:Im9 is highly avid with an off-rate of the order of days.  This avidity poses a biological paradox in that tight binding is required to prevent host cell death, yet rapid release is required for cell invasion and intoxication.  In my seminar I will describe how single molecule dynamic force spectroscopy experiments (DFS) coupled with protein engineering and ensemble methods on a range of proteins and their complexes has been used to resolve this paradox.

Location: Dianna Bowles Lecture Theatre, Department of Biology

Admission: All Welcome