Confocal microscopy
Our confocal microscopy area consists of five different confocal microscopes with the aim to cover as many techniques as possible. All microscopes are in temperature controlled rooms with technical help always nearby. The labs are well equipped and available to both internal and external users. Confocal room 1 (B/K035) also has live-cell imaging equipment which includes an in-room incubator for overnight housing of cells, stage incubator system for full climate control on stage which includes a perfusion option. Micromanipulators are also readily available for the addition of solutions e.g. chemical, protein, DNA or RNA.
This is used extensively for many sample types, including live cell studies, tissue sections and morphological studies of other substrates, with or without the aid of fluorophores. It enables colocalisation with multiple fluorescent probes and advanced techniques such as FRET, FRAP and Spectral Unmixing.
Confocal 1 - Zeiss LSM 880 with Airyscan on an Axio Observer.Z1 invert (B/K035)
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Zeiss LSM 880 invert |
Confocal 2 - Zeiss LSM 780 multiphoton on an Axio Observer.Z1 invert (B/K029)
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Zeiss LSM 780 multiphoton |
Confocal 3 - Zeiss LSM 710 on an AxioImager.M2 (B/K034)
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Zeiss LSM 710 Upright |
Confocal 4 - Zeiss LSM 510 meta on an Axiovert 200M (B/K037)
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Zeiss LSM 510 meta invert Zeiss on-stage incubation system |
Instrument | Lasers |
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Confocal 1 - Zeiss LSM 880 invert (B/K035) | 405, 458, 488, 514, 561 and 633 nm |
Confocal 2 - Zeiss LSM 780 multiphoton invert (B/K029) | 405, 458, 488, 514, 561 and 633 nm, Ti:Sa IR Laser 690-1040nm |
Confocal 3 - Zeiss LSM 710 meta upright (B/K034) | 405, 458, 488, 514, 561, 594 and 633 nm |
Confocal 4 - Zeiss LSM 510 meta invert (B/K034) | 458, 477, 488, 514, 543 and 633 nm |
We have several software packages for image analysis. These include Zeiss VisArt, physiological and deconvolution software and PerkinElmer Volocity. For more information, please contact us.