The Imaging and Cytometry Laboratory is exploring uses of a JEOL JASM-6200 ClairScope atmospheric scanning electron microscope, enabling SEM observation of specimens at normal atmospheric pressure.
The JEOL ClairScope is the first commercially available dual light microscope and electron
microscope. Samples are
presented on a unique petri dish with an integral silicon nitride (SiN) membrane. Real-time fluorescence
imaging of live samples grown or mounted on the petri dish is achieved using an
upright Olympus Microscope. The sample can then be fixed and stained in situ for simple and accurate correlated imaging through the SiN membrane using the JEOL inverted SEM.
We are investigating a number of chemical and biological applications to learn how best to use this innovative instrument, and optimise the EM staining.
A549 lung carcinoma cells: Fluorescence: lipid raft marker, Cholera toxin b-Alexa488 SEM (2500x) of lamella of left cell after Pt blue staining