Flow Cytometry Course 23-26 January 2018

Tuesday 23 January 2018, 9.30AM to Fri 23 Jun 2017

An intensive, hands-on, four-day training course in the varied techniques using flow cytometry. The course will initially cover basic flow cytometry but then rapidly move on to the more advanced applications. These will include cell cycle, apoptosis, cell proliferation, GFP and other fluorescent proteins, FRET and receptor quantification. The course has been designed to allow the participant to gain experience of what a flow cytometer is capable and to raise awareness of the problems that can be encountered. To demonstrate each technique, a wide range of both samples (plants to cell culture) and labels (antibody to fluorescent proteins) will be used. Participants are welcome to bring their own samples.

Introduction

The course is taught by academic and research staff of the University of York Technology Facility, guest specialists from over the UK as well as Beckman Coulter's own applications specialist. Guest specialists will give an initial talk on their specialised techniques and will then participate in the running of the practical sessions.

The University of York is pleased to present the course in collaboration with Beckman Coulter.

Based on delegates from similar courses in 2014 and 2015, it is anticipated that participants will come from both academia and industry throughout the UK, Europe and the rest of the world.

The Course

This intensive, four-day training course will be of value to anyone wanting to gain hands-on experience of modern flow cytometric techniques. In addition to the hands-on component, the course will include tutorials on the theory and practice of all the techniques and methods covered, as well as many other relevant topics in the field.

An essential feature of our course is the unique combination of practical, laboratory-based work with both taught and tutorial-style classroom sessions. The Technology Facility labs are extremely well equipped for modern flow cytometry, including the two CyAn ADP analysers, with 3 lasers (405, 488 and 635nm) and 9 colour detectors. Participants will have the opportunity to analyse their own samples during the free format session or to cover previous work where appropriate.

Course Programme

The course schedule will involve a tutorial each morning on the techniques relevant to the day's practicals.

Hands-on laboratory activities will include:

  • Operation of a flow cytometer
  • Multicolour flow and compensation
  • Cell Cycle, ploidy and apoptosis
  • Fluorescent Proteins
  • Cell Proliferation
  • Receptor Quantification
  • FRET - fluorescent resonance energy transfer

Derek Davies (CRICK, Lincoln's Inn Fields) will be guest speaker and will demonstrate on Wednesday.

There will be ample opportunities during the evenings for further discussion with the course tutors in more informal surroundings.

A preliminary programme is shown below: 

DayAims
Day 1: Basics Familiarisation, multicolour flow and compensation
Day 2: DNA assays Cell Cycle, Ploidy, Apoptosis, Live-Dead
Day 3: Cell and protein analyses Cell proliferation, receptor quantification
Day 4: Fluorescent Proteins studies FRET and Cell sorting demo

Course Tutors

  • Peter O'Toole, Technology Facility, Department of Biology, University of York
  • Derek Davies, CRICK, Lincoln's Inn Fields
  • Karen Hodgkinson, Technology Facility, Department of Biology, University of York
  • Karen Hogg, Technology Facility, Department of Biology, University of York
  • Jo Marrison, Technology Facility, Department of Biology, University of York
  • Graeme Park, Technology Facility, Department of Biology, University of York
  • Ian Brotherick, Beckman Coulter

Venue

Registration is now open for these courses.

The courses will use the resources and instrumentation of the Technology Facility, which is part of the Department of Biology and is housed in a newly-completed, £25M building.

Accommodation

Delegates will be housed in single en-suite rooms on the University of York campus, adjacent to the Biology Department.

Pre and post course accommodation on campus is available, provided sufficient notice is given.

Course Fee

The registration fee covers accommodation on Tuesday, Wednesday and Thursday nights, meals (except Wednesday evening), refreshments, tuition, laboratory consumables, a bound copy of all course notes, and a special course dinner on the Thursday evening. Delegates are free to make their own arrangements on Wednesday evening, either in a dining room or snack bar on campus or in the historic city of York.

Delegates who register early may do so at a discounted rate.  Please see  for course fees and deadline for early discount.

Registration

As the number of places is limited to only twelve to permit full hands-on practice, early registration is advised. To register for the Flow Cytometry Hands-on Course, please complete your form and return to: 

Course Enquiries
Bioscience Technology Facility
Department of Biology (Area 15)
University of York
York YO10 5DD
UK
Tel: +44 (0)1904 328821
Fax: +44 (0)1904 328804
E-mail:  btf-enquiries@york.ac.uk

Refund Policy

Following the volcanic eruptions in Iceland in 2010,  several delegates had travel plans disrupted and were unable to attend a course.  This has prompted us to remind delegates that the full registration fees are payable in advance and that there is a 10% administrative charge for cancellations received in writing up to 20 working days before the start of the course.  No refunds will be made for cancellations received within 20 working days of the course start date or for the inability to attend the course for whatever reasons. The University is not liable for non-attendance due to travel disruptions,health problems or any other reason that might lead to a delegate not being able to attend the course.  You are strongly advised to ensure you have appropriate insurance.  Substitutions may, of course, be made at any time, providing you inform us in writing. 

More Information

For any questions concerning the course itself, please contact:

Dr Peter O'Toole
Department of Biology (Area 15)
University of York
York YO10 5DD
UK
Tel: +44 (0)1904 328722
Fax: +44 (0)1904 328804
E-mail: peter.otoole@york.ac.uk

 York and the University

The University of York enjoys a splendid campus location just two miles from the centre of York itself. There are excellent rail links from London, and Manchester and Newcastle Airports, and the campus is easily reached by road via the A1 and A64.

This historic walled city with its medieval guild halls and hostelries offers many attractions for the visitor, including the Jorvik Viking Centre, the National Railway Museum, York Minster, York Castle Museum, Clifford's Tower and the Merchant Adventurers' Hall. York is set in the centre of the Vale of York with the Yorkshire Dales, Moors and Wolds, and stately homes and other attractions are all within easy reach. 

 

Previous Course Feedback

"An excellent course with fantastic tutors"

"Thanks for all the team who were really special and what can I say – never better"

"I am very impressed with the course staff, interactions, course material and the way it was planned"

"Excellent course, excellent teaching, Thanks you!"

"A very well run, enjoyable and informative course. I would definitely recommend to future users. I wish I had taken this course a few years ago"

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Location: University of York