David’s lab explores the biology of adult blood stem cells and the process by which single blood stem cells are subverted to drive blood cancers such as leukaemia. David earned a BSc in Genetics and English Literature at the University of Western Ontario, Canada and obtained his PhD in Genetics at the University of British Columbia, Canada. His postdoctoral research was at the University of Cambridge where he primarily studied malignant blood stem cell biology and established his research group in 2015. In 2019, the lab relocated to the University of York and the York Biomedical Research Institute.
One of the simplest and most provocative concepts in all of stem cell biology is how a single stem cell can give rise to any of the highly specialised cell types of a given tissue while also having the capacity to make a new equally potent stem cell. At a population level, this decision making process must exist in a tightly regulated balance in order to avoid tissue degeneration (too few stem cells) or progression to cancer (too many stem cells).
Our research is highly interdisciplinary with three major themes:
Understanding the molecular regulators of normal blood stem cells
Here we focus on how cell fate decisions are made on a single cell level in an effort to understand how to expand stem cell populations outside the body (for cell replacement or as a cell source for gene therapy). We use tools such as single cell RNA-sequencing, clonal tracking and single cell transplantation assays to link molecular profile with cellular function.
Clonal evolution of cancers from single cells
The early stages of cancer evolution from single cells are observable in haematological malignancies such as myeloproliferative neoplasms and bone marrow failure syndromes. Here we utilise mouse models and primary patient samples to study the molecular and cellular function of mutant versus non-mutant cells to understand clonal competition and to identify potential new therapeutic targets. We also study the role of the immune cell microenvironment in disease evolution.
Physical and mechanical biology
The newest area of our lab explores the physical biology of stem cells including the development of new tools/approaches for expanding blood stem cells outside the body. We are exploring the physical and quantitative biology of stem cells through mechanical signalling, mathematical modelling, and the construction of 3D microenvironments and microfluidic devices. This project is funded by the European Research Council.
Current lab members
Our lab is always interested in recruiting talented and motivated scientists; please do not hesitate to get in touch at firstname.lastname@example.org.
One specific funded opportunity for prospective PhD students can be found on the FindAPhD website.
We are currently advertising for a research assistant/technician - applications close 10 November!
I have a long history of public engagement and outreach including the creation of The Black Hole, a website and blog that provides information on and analysis of issues related to the education and training of scientists. I also was course director for Hematology 101, a series of web-based lectures on the basics of blood stem cells and their clinical applications.
I have given lectures at the University of Cambridge and Imperial College London in the areas of stem cell biology, blood cancers, and regenerative medicine. I have not yet started lecturing in York, but would always welcome those interested in these areas to get in touch.
I have experience in problem-based learning (UBC Medical School) and supervision style tutorials (University of Cambridge). Small, focused and regular group discussion is an excellent way to encourage new lines of thinking that reach beyond the core curriculum.
We are always interested in hearing from bright and motivated young scientists and the interdisciplinary nature of many of our projects means that students from many different areas can find something interesting to pursue in our lab. Overall, we focus on blood stem cell biology but our work encompasses cellular and molecular biology, cancer evolution, mouse models, physical and mechanical biology, computational biology and bioinformatics.