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|2010 -||Lecturer||Department of Biology, University of York|
|2003 - 2009||BBSRC David Phillips Fellow||University of Cambridge|
|2000 - 2003||Post-Doc||University of Cambridge|
|1997 - 2000||Post-Doc||MRC Laboratory of Molecular Biology|
|1997||PhD||MRC Laboratory of Molecular Biology|
|1993||MPhil||University of Cambridge|
|1992||MSc||Jawaharlal Nehru University, India|
|1990||BSc (Hons)||University of Delhi, India|
Disability Liaison Officer
Member: Graduate Studentship Committee
Member: Admissions Team, Biomedical Sciences UCAS
Member: Biomedical Sciences Degree Development Group
Research in the laboratory is aimed at understanding how neuronal transcription factors respond to growth factors and synaptic activity to direct gene expression programs underlying neuronal differentiation, neuronal growth and synaptic plasticity. We are specifically interested in the regulation of CREB, serum response factor, Elk-1, Class IIa HDACs and the myocyte enhancer factor-2 family of proteins in rodent hippocampal neurons and in differentiating neural progenitors. We are using Drosophila to investigate the role of these transcriptional regulators in learning and memory and in generating rhythmic behaviours such as circadian rhythms of locomotor activity. One aim of our work is to investigate changes in transcription factor regulation in neurodegenerative diseases that are associated with transcriptional dysfunction.
Regulation of histone-modifying proteins: A major contribution of our research is the finding that synaptic activity-induced signalling pathways can regulate transcriptional coactivators and transcriptional corepressors. My early work identified CBP, a histone acetyl transferase, as a target for neuronal signalling pathways -the first report of signal-dependent regulation of a chromatin-modifying enzyme. Since then, other coactivators and corepressors, such as HDACs, have been reported as targets of signalling cascades. We have shown synaptic activity-dependent nucleocytoplasmic shuttling of HDAC4 and HDAC5 in hippocampal neurons. More recently, we have shown that Class IIa histone deacetylases are conserved regulators of circadian function in mammalian cells and in Drosophila.
Differential effects of signalling pathways: We discovered that while MEF2 transcription factors are activated by Ca2+ signalling, cAMP acts to repress MEF2 factors. We have also identified differential effects of calcineurin, a phosphatase that acts to constrain hippocampal synaptic plasticity, on CREB and SRF/Elk1 transcription factors.
Iain Hartnell (MRC funded, with Miles Whittington)
|Brain rhythms and molecular markers of plasticity (MRC funded, with Miles Whittington).|
|Postdoctoral Research Associate||
Christopher Ugbode (BBSRC funded, with Sean Sweeney)
|Reactive Oxygen Species, metabolic by-products of mitochondrial respiration|
|PhD Student||Jack Munns (funded by a Departmental Studentship, with Seth Davis)||Solving a worm's clock: Conserved transcriptional and non-transcriptional cellular mechanisms|
|Postdoctoral Research Associate||Ming Yang||Intravital multiphoton imaging of the brain: integrating immunology, neuroscience and cancer biology|
Role of redox-signalling and oxidative stress in the regulation of neuronal transcription factors (2016-17)
Synaptic activity-induced changes in neuronal gene expression programs direct neuronal differentiation during development and alter connectivity of adult neurons in response to emotional and sensory stimuli. The Class IIa HDACs, HDAC4 and HDAC5 are transcriptional co-repressors that regulate the activity of the myocyte enhancer factor-2 (MEF2) family of transcription factors. Under basal conditions Class IIa HDACs reside in the nucleus and repress MEF2 transcriptional activity. Derepression of MEF2 factors involves synaptic activity-induced nuclear export of Class IIa HDACs through phosphorylation of two conserved serine residues in their N-termini. Recently, reactive oxygen species (ROS) have been implicated in mediating nuclear export of Class IIa HDACs in muscle through the oxidation of conserved cysteines in HDAC4 and HDAC5. Moreover, HDAC5 displays phosphorylation-independent cyclical changes in subcellular localization (Fogg et al, 2014). This PhD project will examine whether ROS signalling influences HDAC4/5 subcellular localization in primary hippocampal neurons in response to synaptic activity and in response to oxidative stress. It will investigate whether there is an interaction between HDAC4/5 cysteine oxidation and phosphorylation. The project will employ a range of cellular, molecular and biochemical techniques to assess HDAC4/5 localization, phosphorylation and cysteine oxidation in neurons.
FoggPCM, O'NeillJS, DobrzyckiT, CalvertS, LordE, LordRL, ElliottCJH, SweeneyST, HastingsMHand Chawla S (2014). Class IIa histone deacetylases are conserved regulators of circadian function. Journal of Biological Chemistry 289, 34341-34348.