Publications by subject

 

Urothelial cell and tissue biology

2018
Baker SC, Arlt VM, Indra R, Joel M, Stiborova M, Eardley I, Ahmad N, Otto W, Burger M, Rubenwolf P, Phillips DH, Southgate J. Differentiation-associated urothelial cytochrome P450 oxidoreductase predicates the xenobiotic-metabolising activity of "luminal" muscle-invasive bladder cancers. Mol Carcinog. 2018 Jan 11. doi: 10.1002/mc.22784. PubMed PMID: 29323757.

Darlow JM, Darlay R, Dobson MG, Stewart A, Charoen P, Southgate J, Baker SC, Xu Y, Hunziker M, Lambert HJ, Green AJ, Santibanez-Koref M, Sayer JA, Goodship THJ, Puri P, Woolf AS, Kenda RB, Barton DE, Cordell HJ. Publisher Correction: Genome-wide linkage and association study implicates the 10q26 region as a major genetic contributor to primary nonsyndromic vesicoureteric reflux. Sci Rep. 2018 Jan 8;8(1):459. doi: 10.1038/s41598-017-17992-w. PubMed PMID: 29311702; PubMed Central PMCID: PMC5758511. 

Hustler A, Eardley I, Hinley J, Pearson J, Wezel F, Radvanyi F, Baker SC, Southgate J. Differential transcription factor expression by human epithelial cells of buccal and urothelial derivation. Exp Cell Res. 2018 Aug 15;369(2):284-294. doi: 10.1016/j.yexcr.2018.05.031. Epub 2018 May 26. PubMed PMID: 29842880; PubMed Central PMCID: PMC6092173.

2017
Ali ASM, Mowbray C, Lanz M, Stanton A, Bowen S, Varley CL, Hilton P, Brown K, Robson W, Southgate J, Aldridge PD, Tyson-Capper A, Abraham S, Pickard RS, Hall J. Targeting deficiencies in the TLR5 mediated vaginal response to treat female recurrent urinary tract infection. Sci Rep. 2017. PMID: 28887442.

Fishwick C, Higgins J, Percival-Alwyn L, Hustler A, Pearson J, Bastkowski S, Moxon S, Swarbreck D, Greenman CD, Southgate J. Heterarchy of transcription factors driving basal and luminal cell phenotypes in human urothelium.
Cell Death Differ. 2017; 24(5); 809-818. PMID: 28282036.

2016
Baker SC, Shabir S, Georgopoulos NT, Southgate J. Ketamine-Induced Apoptosis in Normal Human Urothelial Cells: A Direct, N-Methyl-d-Aspartate Receptor-Independent Pathway Characterized by Mitochondrial Stress.
Am J Pathol. 2016; 186(5); 1267-77. PMID: 27001627.

Kidger E, Stahlschmidt J, Garthwaite M, Fulford S, Southgate J, Baker SC. A rare urachal cyst in a case of ketamine-induced cystitis provides mechanistic insights. Urology 2015; 223.el-7. PMID:  26743387. 

Zhang Z, Bedder M, Smith SL, Walker D, Shabir S, Southgate J. Characterization and classification of adherent cells in monolayer culture using automated tracking and evolutionary algorithms. Biosystems. 2016; 146:110-21. PMID: 27267455. 

2015
Smith NJ, Hinley J, Varley CL, Eardley I, Trejdosiewicz LK, Southgate J. The human urothelial tight junction:  claudin 3 and the ZO-1α+ switch. Bladder 2015.  PMID: 26269793.

2014
Baker SC, Shabir S, Southgate J. Biomimetic urothelial tissue models for the in vitro evaluation of barrier physiology and bladder drug efficacy. Mol Pharm. 2014; 11(7):1964-70.  PMID: 24697150. (review). 

Böck M, Hinley J, Schmitt C, Wahlicht T, Kramer S, Southgate J. Identification of ELF3 as an early transcriptional regulator of human urothelium. Dev Biol. 2014; 386(2):321-30. PMID: 24374157. 

Georgopoulos NT, Kirkwood LA, Southgate J.  A novel bidirectional positive feedback loop between Wnt/β-catenin and EGFR/ERK: role of context-specific signalling crosstalk in modulating epithelial tissue regeneration. J Cell Science 2014; 127(pt 13): 2967-82.  PMID 24816560.  

Wezel F, Pearson J, Southgate J. Plasticity of in vitro-generated urothelial cells for functional tissue formation. Tissue Eng Part A. 2014; 20(9-10): 1358-68. PMID: 24350594.

2013
Baker SC, Stahlschmidt J, Oxley J, Hinley J, Eardley I, Marsh F, Gillatt D, Fulford S, Southgate J. Nerve hyperplasia: a unique feature of ketamine. Acta Neuropathologica Comm 2013;1(64):1-7. PMID: 24252413.

Garthwaite M, Hinley J, Cross W, Warwick RM, Ambrose A, Hardaker H, Eardley I, Southgate J. Use of donor bladder tissues for in vitro research. BJU Int. 2013; 113(1): 160-6. PMID: 24053725.

Shabir S, Cross W, Kirkwood LA, Pearson JF, Appleby PA, Walker D, Eardley I, Southgate J. Functional expression of purinergic P2 receptors and transient receptor potential channels by the human urothelium. Am J Physiol Renal Physiol. 2013;305(3):F396-406. PMID: 23720349.

Wezel F, Southgate J. Reprogramming stromal cells from the urinary tract and prostate: a trip to pluripotency and back? Eur Urol. 2013;64(5):762-4. PMID: 23706568.

2012
Fleming JM, Shabir S, Varley CL, Kirkwood LA, White A, Holder J, Trejdosiewicz LK, Southgate J. Differentiation-associated reprogramming of the transforming growth factor β receptor pathway establishes the circuitry for epithelial autocrine/paracrine repair. PLoS One. 2012;7(12):e51404. PMID: 23284691.

Rubenwolf PC, Georgopoulos NT, Kirkwood LA, Baker SC, Southgate J. Aquaporin expression contributes to human transurothelial permeability in vitro and is modulated by NaCl. PLoS One. 2012;7(9):e45339. PMID: 23028946.

2011
Georgopoulos NT, Kirkwood LA, Varley CL, Unwin RS, MacLaine N, Aziz N, Southgate J. 2011.  Immortalisation of normal human urothelial cells compromises differentiation.  Eur Urol. 60, 141-9.

Munkley J, Copeland NA, Moignard V, Knight JR, Greaves E, Ramsbottom SA, Pownall ME, Southgate J, Ainscough JF, Coverley D. 2011. Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells. Nucleic Acids Res. 39 (7), 2671-7.

Rubenwolf P, Southgate J. 2011.  Permeability of differentiated human urothelium in vitro.  Permeability Barrier – Methods and Protocols, Walker (Ed):  Springer. 763: 207.

Smith NJ, Varley CL, Eardley I, Feather S, Trejdosiewicz L, Southgate J.  2011. Toll-Like receptor responses of normal human urothelial cells to bacterial flagellin and lipopolysaccharide.  J Urol. 186, 1084-1092.

Varley CL, Southgate J.  2011. Organotypic and 3D reconstructed cultures of the human bladder and urinary tract. Methods Mol Biol. 695, 197-211.  

Wood D, Cottrell A, Baker SC, Southgate J, Harris M, Fulford S, Woodhouse C, Gillatt D.  2011.  Recreational ketamine:  from pleasure to pain.  BJU.  107(12): 1881-4.

2010
Georgopoulos NT, Kirkwood LA, Walker DC, Southgate J.  2010.   Differential regulation of growth-promoting signaling pathways by E-cadherin. PLoS ONE.  5, e13621.

2009
Chopra B, Georgopoulos NT, Nicholl A, Hinley J, Oleksiewicz MB, Southgate J. 2009. Structurally diverse peroxisome proliferator-activated receptor agonists induce apoptosis in human uro-epithelial cells by a receptor-independent mechanism involving store-operated calcium channels. Cell Prolif. 42, 688-00.

Rubenwolf PC, Georgopoulos NT, Clements LA, Feather S, Holland P, Thomas DF, Southgate J. 2009. Expression and Localisation of Aquaporin Water Channels in Human Urothelium In Situ and In Vitro. Eur Urol. 56, 1013-1024.

Varley CL, Bacon EJ, Holder JC, Southgate J. 2009. FOXA1 and IRF-1 intermediary transcriptional regulators of PPARgamma-induced urothelial cytodifferentiation. Cell Death Differentiation 16, 103-114.

2008
Chopra B, Hinley J, Oleksiewicz MB, Southgate J. 2008. Trans-species comparison of PPAR and RXR expression by rat and human urothelial tissues. Toxicol Pathol 36, 485-495.

MacLaine NJ, Wood MD, Holder JC, Rees RW, Southgate J. 2008. Sensitivity of normal, paramalignant, and malignant human urothelial cells to inhibitors of the epidermal growth factor receptor signaling pathway. Mol Cancer Res 6, 53-63.

Marsh F, Garthwaite MA, Southgate J, Ramage C. 2008. Assessing UK specialists' knowledge of the diagnostic criteria for painful bladder syndrome/interstitial cystitis. Int Urogynecol J Pelvic Floor Dysfunct 19, 615-62.

Shabir S, Southgate J, 2008. Calcium signalling in wound-responsive normal human urothelial cell monolayers. Cell Calcium 44, 453-464.

Varley CL, Southgate J. 2008. Effects of PPAR agonists on proliferation and differentiation in human urothelium. Exp Toxicol Pathol 60, 435-441. (review).

2007
Southgate J, Varley CL, Garthwaite MA, Hinley J, Marsh F, Stahlschmidt J, Trejdosiewicz LK, Eardley I. 2007. Differentiation potential of urothelium from patients with benign bladder dysfunction. BJU Int 99, 1506-1516.

2006
Garthwaite MA, Thomas DFM, Subramaniam R, Stahlschmidt J, Eardley I, Southgate J. 2006. Urothelial differentiation in vesicoureteric reflux and other urological disorders of childhood: a comparative study. Eur Urol 49, 154-159, 159-160.

Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK. 2006. A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation. Cell Death Differ 13, 1789-1801.

Hall GD, Smith B, Weeks RJ, Selby PJ, Southgate J, Chester JD. 2006. Novel urothelium specific gene expression identified by differential display reverse transcriptase-polymerase chain reaction. J Urol 175, 337-342.

Jenkins D, Bitner-Glindzicz M, Malcolm S, Allison J, de Bruyn R, Flanagan S, Thomas DFM, Belk RA, Feather SA, Bingham C, Southgate J, Woolf AS. 2006. Mutation analyses of Uroplakin II in children with renal tract malformations. Nephrol Dial Transplant 21, 3415-3421.

Steele LP, Georgopoulos NT, Southgate J, Selby PJ, Trejdosiewicz LK. 2006. Differential susceptibility to TRAIL of normal versus malignant human urothelial cells. Cell Death Differ 13, 1564-1576.

Varley CL, Garthwaite MA, Cross W, Hinley J, Trejdosiewicz LK, Southgate J. 2006. PPARgamma-regulated tight junction development during human urothelial cytodifferentiation. J Cell Physiol 208, 407-417.

Cancer research

2018
Baker SC, Arlt VM, Indra R, Joel M, Stiborova M, Eardley I, Ahmad N, Otto W, Burger M, Rubenwolf P, Phillips DH, Southgate J. Differentiation-associated urothelial cytochrome P450 oxidoreductase predicates the xenobiotic-metabolising activity of "luminal" muscle-invasive bladder cancers. Mol Carcinog. 2018 Jan 11. doi: 10.1002/mc.22784. PubMed PMID: 29323757.

Hustler A, Eardley I, Hinley J, Pearson J, Wezel F, Radvanyi F, Baker SC, Southgate J. Differential transcription factor expression by human epithelial cells of buccal and urothelial derivation. Exp Cell Res. 2018 Aug 15;369(2):284-294. doi: 10.1016/j.yexcr.2018.05.031. Epub 2018 May 26. PubMed PMID: 29842880; PubMed Central PMCID: PMC6092173.

2016
Dunnill CJ, Ibraheem K, Mohamed A, Southgate J, Georgopoulos NT. A redox state-dictated signalling pathway deciphers the malignant cell specificity of CD40-mediated apoptosis. Oncogene. 2016 Nov 21. doi: 10.1038/onc.2016.401. PMID: 27869172.

2014
Biton A, Bernard-Pierrot I, Lou Y, Krucker C, Chapeaublanc E, Rubio-Pérez C, López-Bigas N, Kamoun A, Neuzillet Y, Gestraud P, Grieco L, Rebouissou S, de Reyniès A, Benhamou S, Lebret T, Southgate J, Barillot E, Allory Y, Zinovyev A, Radvanyi F. Independent component analysis uncovers the landscape of the bladder tumor transcriptome and reveals insights into luminal and basal subtypes. Cell Reports. 2014; 9(4):1235-45. PMID: 25456126.

Rebouissou S, Bernard-Pierrot I, de Reyniès A, Lepage M-L, Krucker C, Chapeaublanc E, Hérault A, Kamoun A, Caillaut A, Letouzé E, Elarouci N, Neuzillet Y, Denoux Y, Molinié V, Vordos D, Laplanche A, Maillé P, Soyeux P, Ofualuka K, Reyal F, Biton A, Sibony M, Paoletti X, Southgate J, Benhamou S, Lebret T, Allory Y, Radvanyi F. EGFR as a potential therapeutic target for a subset of muscle-invasive bladder cancers with a basal-like phenotype.  Science Translational Medicine 2014; 6(244).  PMID: 25009231.

Wezel F, Pearson J, Kirkwood LA, Southgate J. Differential expression of Oct4 variants and pseudogenes in normal urothelium and urothelial cancer. Am J Pathol. 2014;183(4):1128-36. PMID: 23933063.

2012
Fleming JM, Shabir S, Varley CL, Kirkwood LA, White A, Holder J, Trejdosiewicz LK, Southgate J. Differentiation-associated reprogramming of the transforming growth factor β receptor pathway establishes the circuitry for epithelial autocrine/paracrine repair. PLoS One. 2012;7(12):e51404. PMID: 23284691.

Ho JR, Chapeaublanc E, Kirkwood L, Nicolle R, Benhamou S, Lebret T, Allory Y, Southgate J, Radvanyi F, Goud B. Deregulation of Rab and Rab effector genes in bladder cancer. PLoS One. 2012;7(6):e39469. PMID: 22724020. 2011 Munkley J, Copeland NA, Moignard V, Knight JR, Greaves E, Ramsbottom SA, Pownall ME, Southgate J, Ainscough JF, Coverley D. 2011. Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells. Nucleic Acids Res. 39 (7), 2671-7.

Vallot C, Stransky N, Bernard-Pierrot I, Herault A, Zucman-Rossi J, Chapeaublanc E, Vordos D, Laplanche A, Benhamou S, Lebret T, Southgate J, Allory Y, Radvanyi Francois. 2011. A novel epigenetic phenotype associated with the most aggressive pathway of bladder tumor progression. J Natl. Cancer Inst. 103, 47-60.

2009
Egerod FL, Svendsen JE, Hinley J, Southgate J, Bartels A, Brunner N, Oleksiewicz MB. 2009. PPAR alpha and PPAR gamma coactivation rapidly induces Egr-1 in the nuclei of the dorsal and ventral urinary bladder and kidney pelvis urothelium of rats. Toxicol Pathol 37, 947-958.

2008
Oleksiewicz MB, Southgate J, Iversen L, Egerod FL. 2008. Rat Urinary Bladder Carcinogenesis by Dual-Acting PPARalpha + gamma Agonists. PPAR Res 2008, 103167.  (review).

2006
Crallan RA, Georgopoulos NT, Southgate J. 2006. Experimental models of human bladder carcinogenesis. Carcinogenesis 27, 374-381.  (review).

Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK. 2006. A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation. Cell Death Differ 13, 1789-1801.

Steele LP, Georgopoulos NT, Southgate J, Selby PJ, Trejdosiewicz LK. 2006. Differential susceptibility to TRAIL of normal versus malignant human urothelial cells. Cell Death Differ 13, 1564-1576.

Stransky N, Vallot C, Reyal F, Bernard-Pierrot I, de Medina SG, Segraves R, de Rycke Y, Elvin P, Cassidy A, Spraggon C, Graham A, Southgate J, Asselain B, Allory Y, Abbou CC, Albertson DG, Thiery JP, Chopin DK, Pinkel D, Radvanyi F. 2006. Regional copy number-independent deregulation of transcription in cancer. Nat Genet 38, 1386-1396.

Tissue Engineering and biomaterials

2018 
Hustler A, Eardley I, Hinley J, Pearson J, Wezel F, Radvanyi F, Baker SC, Southgate J. Differential transcription factor expression by human epithelial cells of buccal and urothelial derivation. Exp Cell Res. 2018 Aug 15;369(2):284-294. doi: 10.1016/j.yexcr.2018.05.031. Epub 2018 May 26. PubMed PMID: 29842880; PubMed Central PMCID: PMC6092173.

2014
Bullers S, Baker S, Ingham E, Southgate J. The human tissue:biomaterial interface: a role for PPARγ-dependent glucocorticoid receptor activation in regulating the CD163+ M2 macrophage phenotype. Tissue Eng Part A. 2014; 20(17-18):  2390-401.  PMID: 24548290. 

Garriboli M, Radford A, Southgate J.  Regenerative medicine in urology.  Eur J Pediatr Surg. 2014; 24(3): 227-36.  PMID: 24918400.

Wezel F, Pearson J, Southgate J. Plasticity of in vitro-generated urothelial cells for functional tissue formation. Tissue Eng Part A. 2014; 20(9-10): 1358-68. PMID: 24350594.

Wezel F, Southgate J.  Tissue engineering using ceramics and polymers (2nd edition).  Tissue/organ regeneration:  the Bladder.  Woodhead Publishing. 2014; 439-467. (book chapter).

2012
Subramaniam R, Turner AM, Abbas SK, Thomas DF, Southgate J. Seromuscular grafts for bladder reconstruction: extra-luminal demucosalisation of the bowel. Urology. 2012;80(5):1147-50. PMID: 23107403.

2011
Aboushwareb T, McKenzie P, Wezel F, Southgate J, Badlani G. 2011. Is tissue engineering and biomaterials the future for lower urinary tract dysfunction (LUTD)/pelvic organ prolapse (POP)? Neurourol Urodyn, 30(5):775-82. Baker SC, Southgate J.  2011. Bladder Tissue Regeneration.  Electrospinning for tissue regeneration, Bosworth & Downes (Eds): Woodhead Publishing Limited, 225-241 (book chapter).

Baker SC, Rohman G, Hinley J, Stahlschmidt J, Cameron NR, Southgate J. 2011.  Cellular integration and vascularisation promoted by a resorbable particulate-leached cross-linked poly(ε-caprolactone) scaffold.  Macromolecular Bioscience.  11(5), 618-27.

Subramaniam R, Hinley J, Stahlschmidt J, Southgate J. 2011. Tissue-Engineering Potential of Urothelial Cells from Abnormal Pediatric Bladders.  J Urol. 186, 2014-2020.

Wezel F, Southgate J, Thomas DFM.  2011.  Regenerative medicine in urology. Br J Urol Int. 108, 1046-1065.

2010
Turner A, Subramanian R, Thomas DFM, Hinley J, Abbas SK, Stahlschmidt J, Southgate J.  2010.  Transplantation of autologous differentiated urothelium in an experimental model of composite cystoplasty.  Eur.Urol. 59, 447-454.

2009
Baker SC, Rohman G, Southgate J, Cameron NR. 2009. The relationship between the mechanical properties and cell behaviour on PLGA and PCL scaffolds for bladder tissue engineering. Biomaterials 30, 1321-1328.

Korossis S, Bolland F, Southgate J, Ingham E, Fisher J. 2009. Regional biomechanical and histological characterisation of the passive porcine urinary bladder: Implications for augmentation and tissue engineering strategies. Biomaterials 30, 266-275.

Rohman G, Baker SC, Southgate J, Cameron NR. 2009.  Heparin functionalisation of porous PLGA scaffolds for controlled, biologically relevant delivery of growth factors for soft tissue engineering.  J. Mater. Chem 19, 9265-9273.

Turner AM, Southgate J. 2009.  The Tissue-engineered Urinary Bladder:  A Regenerative and Developmental Challenge.  Fundamentals of Tissue Engineering and Regenerative Medicine, Meyer et al (Eds): Springer, 429-440. (book chapter).

2008
Baker SC, Southgate J. 2008. Towards control of smooth muscle cell differentiation in synthetic 3D scaffolds. Biomaterials 29, 3357-3366.

Bolland F, Southgate J. 2008. Bio-engineering urothelial cells for bladder tissue transplant. Expert Opin Biol Ther 8, 1039-1049. (review).

Turner AM, Subramaniam R, Thomas DFM, Southgate J. 2008. Generation of a functional, differentiated porcine urothelial tissue in vitro. Eur Urol 54, 1423-1432.

Wood D, Southgate J. 2008. Current status of tissue engineering in urology. Curr Opin Urol 18, 564-569. (review).

2007
Turner AM, Subramanian R, Thomas DFM.  (2007).  Bladder Tissue Engineering. Tissue engineering using ceramics and polymers, Boccaccini & Gough (Eds):  Woodhead Publishing Limited, 445-465 (book chapter).

2006
Baker SC, Atkin N, Gunning PA, Granville N, Wilson K, Wilson D, Southgate J. 2006. Characterisation of electrospun polystyrene scaffolds for three-dimensional in vitro biological studies. Biomaterials 27, 3136-3146.

Korossis S, Bolland F, Ingham E, Fisher J, Kearney J, Southgate J. 2006. Review: tissue engineering of the urinary bladder: considering structure-function relationships and the role of mechanotransduction. Tissue Eng 12, 635-644.  (review).

 

 

 

Computational modelling

2016
Zhang Z, Bedder M, Smith SL, Walker D, Shabir S, Southgate J. Characterization and classification of adherent cells in monolayer culture using automated tracking and evolutionary algorithms. Biosystems. 2016; S0303-2647(16)30072-7. PMID: 27267455. 

2015
Appleby PA, Shabir S, Southgate J, Walker D. Sources of variability in cytosolic calcium transients triggered by stimulation of homogeneous uro-epithelial cell monolayers. J R Soc Interface. 2015; 6;12(105). PMID: 25694543.

2013
Appleby PA, Shabir S, Southgate J, Walker D. Cell-type-specific modelling of intracellular calcium signalling: a urothelial cell model. J R Soc Interface. 2013;10(86):20130487. PMID: 23864504.

2012
Walker DC, Southgate J. The modulatory effect of cell-cell contact on the tumorigenic potential of pre-malignant epithelial cells: a computational exploration. J R Soc Interface. 2012. PMID:  23097504.

2011
Understanding the Dynamics of Biological Systems:  Lessons Learned from Integrative Systems Biology, Dubitzky H, Southgate J, Fuβ H (Eds).  2011.  Springer. (edited book).

2010
Walker D, Georgopoulos NT, Southgate J. 2010. Anti-social cells: Predicting the influence of E-cadherin loss on the growth of epithelial cell populations. J Theor Biol 262, 425-440.

2009
Walker DC, Southgate J. 2009. The virtual cell - a candidate co-ordinator for 'middle-out' modelling of biological systems. Brief Bioinform 10, 450-461. (review).

2008
Walker DC, Georgopoulos NT, Southgate J. 2008. From pathway to population - a multiscale model of juxtacrine EGFR-MAPK signalling. BMC Syst Biol 2, 102.

2007
Bolland F, Korossis S, Wilshaw SP, Ingham E, Fisher J, Kearney JN, Southgate J. 2007. Development and characterisation of a full-thickness acellular porcine bladder matrix for tissue engineering. Biomaterials 28, 1061-1070.

Rohman G, Pettit JJ, Isaure F, Cameron NR, Southgate J. 2007. Influence of the physical properties of two-dimensional polyester substrates on the growth of normal human urothelial and urinary smooth muscle cells in vitro. Biomaterials 28, 2264-2274.

2006
Walker D, Wood S, Southgate J, Holcombe M, Smallwood R. 2006. An integrated agent-mathematical model of the effect of intercellular signalling via the epidermal growth factor receptor on cell proliferation. J Theor Biol 242, 774-789.