Enzyme discovery - Developing a new platform technology for oxidative biocatalysis
Funded by CoEBio3
Professor Neil Bruce (CNAP), Dr Gideon Grogan (YSBL, Department of Chemistry) and Professor Sabine Flitsch (CoEBio3)
This project aims to establish a suite of technologies for the cloning, functional expression and screening of prokaryotic and eukaryotic cytochromes P450 in E. coli. Cytochromes P450 represent a largely untapped resource in the context of industrial hydroxylations. This is essentially due to previous difficulties with the heterologous expression and screening of these P450s in E. coli, and the requirement for auxiliary redox proteins for electron transfer to the important heme domain, which is responsible for substrate specificity. We have recently developed a cloning system – LIC-RED - based on the unusual and naturally fused P450Rhf from Rhodococcus NCIMB 9784, which permits drop-in of heme domains from a variety of sources to generate libraries of redox-self sufficient CYPs, which catalyse substrate oxidation merely with the addition of NADPH.
Secret to making renewable energy from wood? The digestive system of the gribble may hold the key!
2 new Networks in Industrial Biotechnology awarded! Congratulations to Ian Graham and Simon McQueen-Mason, who will each lead a phase II NIBB.
Poppy genome decoded DNA code of the opium poppy genome determined.
Strengthening links with India: 2 major new research projects Funding secured by CNAP PIs.
CNAP Director, Professor Neil Bruce
CNAP, Department of Biology, University of York, Wentworth Way, York YO10 5DD, UK