Partners

Herman Höfte, Lise Jouanin

INRA is the French national institute for agronomic research. Three INRA laboratories in the field of plant cell wall research participate in this proposal. The Laboratory of Cell Biology (lab1) and the UMR 206 AgroParisTech-INRA team (lab2) in the centre of Versailles-Grignon and the INRA Laboratory in Nantes (lab3).

The group of Herman Höfte and Lise Jouanin has been working for over 10 years on the genetic analysis of cell walls in/ Arabidopsis/. Major
discoveries are the genetic identification of key components of the machinery for cellulose and pectin synthesis and the modification of
hemicelluloses, the identification of key genes for lignin synthesis and regulators of cell elongation. They also developed a FT-IR based
classification system for cell wall mutants, which has been adopted by several other laboratories.

In the framework of the RENEWALL project, our group contributes to the screening for novel cell wall and saccharification mutants in
Brachypodium and the cloning of the correspondig genes, the study of the synthesis of cellulose, synthesis and  polymerisation of lignin and the
development of novel strategies to improve saccharification potential of grasses.

Contributors:
Herman Höfte, Lise Jouanin : PIs
Oumaya Bouchabke : tissue culture and transformation of Brachypodium
Richard SIbout : Brachypodium mutant screens and the study of lignification
Gregory Mouille : Cell wall chemistry, FT-IR
Olivier Darraq : Brachypodium mutant screens
Patrick Grillot : Greenhouse work
Madeleine Bouvier : Lignification in Brachypodium

  Brachypodium   
Left to right:
1. An EMS mutageneized collection of Brachypodium distachyon is produced at INRA Versailles.
2. Brachypodium distachyon, Bd21.3, is a small C3 monocot.
3. Cross section of a stem stained with Phloroglucinol-HCl.