Dr Paul Genever
Reader

Profile

Career

 

2013 - Reader Department of Biology, University of York
2008 - 2013 Senior lecturer Department of Biology, University of York
2003 - 2008 Lecturer Department of Biology, University of York
1993 - 2003 ARC Non-Clinical Career Development Fellowship Department of Biology, University of York
1996 - 1999 Post-Doctoral RA Department of Biology, University of York
1993 - 1996 Post-Doctoral RA Department of Biology, Brunel University, West London
1993 PhD Department of Biochemistry and Molecular Biology, University of Leeds
1989 - 1990 Research Officer Department of Chemical Carcinogenesis, Institute of Cancer Research, London
1989 BSc Biochemistry/ Physiology University of Leeds

Research

Overview

Our work is focused on identifying cellular and molecular pathways that regulate differentiated tissue function, primarily in skeletal systems (bone, cartilage, marrow). This requires an understanding of the fundamental signalling mechanisms that determine adult stem cell fate, tissue remodelling, repair and regeneration and how to apply this knowledge to tissue engineering approaches and the treatment of human disease conditions (orthobiologics).

Discoveries

We discovered that the Wnt signalling cascade plays a fundamental role in bone remodelling, influencing both bone resorption and formation. We have identified and characterised several new tissue sources of adult stem cells and are applying these to tissue engineering approaches.

Current projects

  • Design of an optimal cryopreservation protocol for human embryonic stem cells (joint with Prof. David Pegg and Dr. Charles Hunt, UK Stem Cell Bank) (Funding body: MRC)
  • Generation of three dimensional tissue structures with targeted gene deletions (Funding body: Dr. Hadwen Trust)
  • Regulation of mesenchymal stem cell activity in an in vitro model of the stem cell niche (Funding body: BBSRC/Smith and Nephew)
  • Examination of the Wnt signalling pathway during osteogenesis in embryonic and mesenchymal stem cells (Funding body: DFG Fellowship awarded to Dr Tatjana Schilling)
  • Role of FGF signalling in mesenchymal differentiation (Funding body: BBSRC)
  • Wnt signalling in the maintenance of the haematopoietic stem cell niche (Funding body: MRC)
  • Modulators of Wnt signalling in mesenchymal stem cells (Funding body: BBSRC/AtraZeneca)
  • Tissue Engineering of a Functional Vertebral Endplate Construct (Funding body: EPSRC, Joint with Leeds)
  • Towards tissue engineering a joint replacement (Funding body: EPSRC, Joint with Leeds)
  • Development of a novel osteochondral construct ( Funding body: RegeNer8)

Research group(s)

StatusNameProject
Post Doctoral Research Assistant Dr Aixin Cheng Expression profiling and functional analysis of mesenchymal stem cells undergoing osteogenic differentiation
Post Doctoral Research Assistant Dr Satya Varanasi Expression profiling and functional analysis of mesenchymal stem cells undergoing osteogenic differentiation
Post Doctoral Research Assistant Dr Jen Dyson Generation of three dimensional tissue structures with targeted gene deletions
Post Doctoral Research Assistant Dr Tatjana Schilling Regulation of osteogenesis by Wnt signalling using pluripotent stem cells
Research Student Seun Adewumi Design of an optimal cryopreservation protocol for human embryonic stem cells (joint with Prof. David Pegg and Dr. Charles Hunt, UK Stem Cell Bank)
Research Student Sally Jackson Wnt signalling in the maintenance of the haematopoietic stem cell niche (Joint with Dr. Mark Coles)
Research Student Fatima Saleh Regulation of mesenchymal stem cell activity in an in vitro model of the stem cell niche
Research Student Fiona Warrander Role of FGF signalling in mesenchymal differentiation (Joint with Dr. Harv Isaacs)
Research Student David Cook  Modulators of Wnt signalling in mesenchymal stem cells
Research Student Niki Gosling (Joint with Leeds) Tissue Engineering of a Functional Vertebral Endplate Construct
Research Student Will Vickers (Joint with Leeds) Towards tissue engineering a joint replacement.

Available PhD research projects

Analysis of mesenchymal stromal/stem cell sub-populations (2014-15)

Human mesenchymal stromal/stem cells (MSCs) are found in adult tissues such as bone marrow and are able to differentiate into osteogenic, chondrogenic and adipogenic tissues. There is intense interest in determining how MSCs may be used in future cell-based therapies, including gene therapy, immunotherapy and tissue engineering, and as in vitro models for fundamental research and drug discovery. However, little is known about MSC identity and research is often performed on heterogeneous mixtures of different MSC sub-populations. Using a process of telomerase-based immortalisation and cell cloning, we have generated several different MSC lines that represent different “types” of MSCs. For example, some MSC lines demonstrate tri-lineage, whereas other are bi-potent, uni-potent or nulli-potent. This project will examine the differentiation characteristics of these MSC lines to determine how they reflect MSC sub-populations in vivo.

Dr Paul Genever

Contact details

Dr Paul Genever
Reader
Department of Biology (Area 9)
University of York
York
YO10 5DD

Tel: 01904 328649

http://www.york.ac.uk/res/btr/