Profile

Career

Research

Overview

Parasitic protozoa give rise to some of the most widespread human diseases. Our research focuses mainly on the Leishmaniases, caused by species of the kinetoplastid parasite, Leishmania, that live intracellularly in cells of the immune system. Our long-term aims are to understand host/parasite interactions and to develop novel drugs and vaccines. Current projects focus on the functional analyses of infective-stage Leishmania proteins; comparative genomic analysis of Leishmania species that cause different disease tropisms; structure/function studies on N-myristoyl transferase and its downstream targets in Leishmania, Trypanosoma Drucei and the malaria parasite, Plasmodium falciparum.

Discoveries

We were part of the international consortium that sequenced Leishmania species responsible for different types of clinical disease and collaborate with the Sanger Institute and international colleagues to exploit genomic approaches in Leishmania biology. We functionally analysed an essential downstream target of N-myristoyl transferase (NMT) in African trypanosomes and used this information to validate specific enzyme inhibitors in vivo. With colleagues, we identified lead compounds for the development of specific inhibitors of Plasmodium falciparum NMT. These advances have supported development of an integrated translational research project to screen NMT from three parasite species for new small molecule inhibitors suitable for pre-clinical drug development.

Most recently, we have identified proteins that are essential for Leishmania development in its sand fly vector  and are currently investigating the molecular mechanisms involved in this process.

Current projects

• N-myristoyl transferase as a drug target for visceral and cutaneous leishmaniasis (with R. Leatherbarrow and E. Tate (Imperial College London),T. Wilkinson (YSBL)) (Funding body: The Wellcome Trust)
• Kinetoplastid bioinformatics (with M. Carrington (Cambridge), M. Berriman (Sanger Institute), D. Roos (Pennsylvania)) (Funding body: The Wellcome Trust)
• Therapeutic CD8+ T cell-biased vaccines for human visceral leishmanias (with P. Kaye (Pl and Lacey (HYMS) (Funding body: The Wellcome Trust)

Research group(s)

Available PhD research projects

Publications

Full publications list

• Price, H. P., M. R. Hodgkinson, M. H. Wright, E. W. Tate, B. A. Smith, M. Carrington, M. Stark, and D. F. Smith. 2012. A Role for the Vesicle-Associated Tubulin Binding Protein ARL6 (BBS3) in Flagellum Extension in Trypanosoma brucei. Biochimica et Biophysica Acta (BBA). Molecular Cell Research. In press.
• Price, H. P., M. R. Hodgkinson, R. S. Curwen, L. M. Maclean, J. A. Brannigan, M. Carrington, B. A. Smith, D. A. Ashford, M. Stark, and D. F. Smith. 2012. The Orthologue of Sjogren's Syndrome Nuclear Autoantigen 1 (SSNA1) in Trypanosoma brucei Is an Immunogenic Self-Assembling Molecule. PLoS One. 7:e31842.
• Bell, A. S., J. E. Mills, G. P. Williams, J. A. Brannigan, A. J. Wilkinson, T. Parkinson, R. J. Leatherbarrow, E. W. Tate, A. A. Holder, and D. F. Smith. 2012. Selective Inhibitors of Protozoan Protein N myristoyltransferases as Starting Points for Tropical Disease Medicinal Chemistry Programs. PLoS Negl Trop Dis. 6:e1625.
• Maclean, L. M., P. J. O'Toole, M. Stark, J. Marrison, C. Seelenmeyer, W. Nickel, and D. F. Smith. 2012. Trafficking and release of the metacyclic HASPB protein in the kinetoplastid parasite Leishmania. Cell Microbiol 19:1462-5822.
• Maroof, A., N. Brown, B. Smith, M. R. Hodgkinson, A. Maxwell, F. O. Losch, U. Fritz, P. Walden, C. N. Lacey, D. F. Smith, T. Aebischer, and P. M. Kaye. 2012. Therapeutic vaccination with recombinant adenovirus reduces splenic parasite burden in experimental visceral leishmaniasis. J Infect Dis. 205:853-863.
• Logan-Klumpler, F. J., N. De Silva, U. Boehme, M. B. Rogers, G. Velarde, J. A. McQuillan, T. Carver, M. Aslett, C. Olsen, S. Subramanian, I. Phan, C. Farris, S. Mitra, G. Ramasamy, H. Wang, A. Tivey, A. Jackson, R. Houston, J. Parkhill, M. Holden, O. S. Harb, B. P. Brunk, P. J. Myler, D. Roos, M. Carrington, D. F. Smith, C. Hertz-Fowler, and M. Berriman. 2012. GeneDB--an annotation database for pathogens. Nucleic Acids Res. 40:D98-D108.
• Rogers, M. B., J. D. Hilley, N. J. Dickens, J. Wilkes, P. A. Bates, D. P. Depledge, D. Harris, Y. Her, P. Herzyk, H. Imamura, T. D. Otto, M. Sanders, K. Seeger, J. C. Dujardin, M. Berriman, D. F. Smith, C. Hertz-Fowler, and J. C. Mottram. 2011. Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania. Genome Res. 21:2129-2142.
• Ramos, C. S., J. K. Yokoyama-Yasunaka, C. Guerra-Giraldez, H. P. Price, R. A. Mortara, D. F. Smith, and S. R. Uliana. 2011. Leishmania amazonensis META2 protein confers protection against heat shock and oxidative stress. Exp Parasitol. 127:228-237.
• Moore, B., A. J. Miles, C. Guerra-Giraldez, P. Simpson, M. Iwata, B. A. Wallace, S. J. Matthews, D. F. Smith, and K. A. Brown. 2011. Structural basis of molecular recognition of the Leishmania small hydrophilic endoplasmic reticulum-associated protein (SHERP) at membrane surfaces. J Biol Chem. 286:9246-9256.
• Sadlova, J., H. P. Price, B. A. Smith, J. Votypka, P. Volf, and D. F. Smith. 2010. The stage-regulated HASPB and SHERP proteins are essential for differentiation of the protozoan parasite Leishmania major in its sand fly vector, Phlebotomus papatasi. Cell Microbiol. 12:1765-1779.
• Price, H. P., A. Peltan, M. Stark, and D. F. Smith. 2010. The small GTPase ARL2 is required for cytokinesis in Trypanosoma brucei. Mol Biochem Parasitol 173:123-131.
• Frearson, J. A., S. Brand, S. P. McElroy, L. A. Cleghorn, O. Smid, L. Stojanovski, H. P. Price, M. L. Guther, L. S. Torrie, D. A. Robinson, I. Hallyburton, C. P. Mpamhanga, J. A. Brannigan, A. J. Wilkinson, M. Hodgkinson, R. Hui, W. Qiu, O. G. Raimi, D. M. van Aalten, R. Brenk, I. H. Gilbert, K. D. Read, A. H. Fairlamb, M. A. Ferguson, D. F. Smith, and P. G. Wyatt. 2010. N-myristoyltransferase inhibitors as new leads to treat sleeping sickness. Nature. 464:728-732.
• Depledge, D. P., L. M. MacLean, M. R. Hodgkinson, B. A. Smith, A. P. Jackson, S. Ma, S. R. B. Uliana, and D. F. Smith. 2010. Leishmania-Specific Surface Antigens Show Sub-Genus Sequence Variation and Immune Recognition. PLoS Negl Trop Dis 4:e829.
• Beattie, L., A. Peltan, A. Maroof, A. Kirby, N. Brown, M. Coles, D. F. Smith, and P. M. Kaye. 2010. Dynamic imaging of experimental Leishmania donovani-induced hepatic granulomas detects Kupffer cell-restricted antigen presentation to antigen-specific CD8 T cells. PLoS 6:e1000805.
• Tournaviti, S., E. S. Pietro, S. Terjung, T. Schafmeier, S. Wegehingel, J. Ritzerfeld, J. Schulz, D. F. Smith, R. Pepperkok, and W. Nickel. 2009. Reversible phosphorylation as a molecular switch to regulate plasma membrane targeting of acylated SH4 domain proteins. Traffic. 10:1047-1060.
• Price, H. P., L. MacLean, J. Marrison, P. J. O'Toole, and D. F. Smith. 2009. Validation of a new method for immobilising kinetoplastid parasites for live cell imaging. Mol Biochem Parasitol 169:66-69.
• Price, H. P., M. L. Guther, M. A. Ferguson, and D. F. Smith. 2009. Myristoyl-CoA:protein N-myristoyltransferase depletion in trypanosomes causes avirulence and endocytic defects. Mol Biochem Parasitol 169:55-58.
• Depledge, D. P., K. J. Evans, A. C. Ivens, N. Aziz, A. Maroof, P. M. Kaye, and D. F. Smith. 2009. Comparative Expression Profiling of Leishmania: Modulation in Gene Expression between Species and in Different Host Genetic Backgrounds. PLoS Negl Trop Dis. 3:e476.
• Brannigan, J. A., B. A. Smith, Z. Yu, A. M. Brzozowski, M. R. Hodgkinson, A. Maroof, H. P. Price, F. Meier, R. J. Leatherbarrow, E. W. Tate, D. F. Smith, and A. J. Wilkinson. 2009. N-Myristoyltransferase from Leishmania donovani: Structural and Functional Characterisation of a Potential Drug Target for Visceral Leishmaniasis. J Mol Biol 396:985-999.
• Aslett, M., C. Aurrecoechea, M. Berriman, J. Brestelli, B. P. Brunk, M. Carrington, D. P. Depledge, S. Fischer, B. Gajria, X. Gao, M. J. Gardner, A. Gingle, G. Grant, O. S. Harb, M. Heiges, C. Hertz-Fowler, R. Houston, F. Innamorato, J. Iodice, J. C. Kissinger, E. Kraemer, W. Li, F. J. Logan, J. A. Miller, S. Mitra, P. J. Myler, V. Nayak, C. Pennington, I. Phan, D. F. Pinney, G. Ramasamy, M. B. Rogers, D. S. Roos, C. Ross, D. Sivam, D. F. Smith, G. Srinivasamoorthy, C. J. Stoeckert, Jr., S. Subramanian, R. Thibodeau, A. Tivey, C. Treatman, G. Velarde, and H. Wang. 2009. TriTrypDB: a functional genomic resource for the Trypanosomatidae. Nucleic Acids Res 38:20.
• Heal, W. P., S. R. Wickramasinghe, P. W. Bowyer, A. A. Holder, D. F. Smith, R. J. Leatherbarrow, and E. W. Tate. 2008. Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry. Chem Commun (Camb).480-482.
• Bowyer, P. W., E. W. Tate, R. J. Leatherbarrow, A. A. Holder, D. F. Smith, and K. A. Brown. 2008. N-Myristoyltransferase: a Prospective Drug Target for Protozoan Parasites. ChemMedChem. 3:402-408.
• Beattie, L., K. J. Evans, P. M. Kaye, and D. F. Smith. 2008. Transgenic Leishmania and the immune response to infection. Parasite Immunol. 30:255-266.
• Smith, D. F., C. S. Peacock, and A. K. Cruz. 2007. Comparative genomics: From genotype to disease phenotype in the leishmaniases. Int J Parasitol. 37:1173-1186.
• Price, H. P., M. Stark, and D. F. Smith. 2007. Trypanosoma brucei ARF1 Plays a Central Role in Endocytosis and Golgi-Lysosome Trafficking. Mol Biol Cell. 18:864-873.
• Price, H. P., M. Stark, B. Smith, and D. F. Smith. 2007. TbARF1 influences lysosomal function but not endocytosis in procyclic stage Trypanosoma brucei. Mol Biochem Parasitol. 155:123-127.
• Peacock, C. S., K. Seeger, D. Harris, L. Murphy, J. C. Ruiz, M. A. Quail, N. Peters, E. Adlem, A. Tivey, M. Aslett, A. Kerhornou, A. Ivens, A. Fraser, M. A. Rajandream, T. Carver, H. Norbertczak, T. Chillingworth, Z. Hance, K. Jagels, S. Moule, D. Ormond, S. Rutter, R. Squares, S. Whitehead, E. Rabbinowitsch, C. Arrowsmith, B. White, S. Thurston, F. Bringaud, S. L. Baldauf, A. Faulconbridge, D. Jeffares, D. P. Depledge, S. O. Oyola, J. D. Hilley, L. O. Brito, L. R. Tosi, B. Barrell, A. K. Cruz, J. C. Mottram, D. F. Smith, and M. Berriman. 2007. Comparative genomic analysis of three Leishmania species that cause diverse human disease. Nat Genet. 39:839-847.
• Moreno, J., J. Nieto, S. Masina, C. Canavate, I. Cruz, C. Chicharro, E. Carrillo, S. Napp, C. Reymond, P. M. Kaye, D. F. Smith, N. Fasel, and J. Alvar. 2007. Immunization with H1, HASPB1 and MML Leishmania proteins in a vaccine trial against experimental canine leishmaniasis. Vaccine. 25:5290-5300.
• Mills, E., H. P. Price, A. Johner, J. E. Emerson, and D. F. Smith. 2007. Kinetoplastid PPEF phosphatases: dual acylated proteins expressed in the endomembrane system of Leishmania. Mol Biochem Parasitol. 152:22-34.
• Escaron, C. J., D. M. Lees, R. Tewari, D. F. Smith, and E. Caron. 2007. A simple, robust and versatile method to characterise intracellular parasitism. Mol Biochem Parasitol. 153:72-76.
• Depledge, D. P., R. P. Lower, and D. F. Smith. 2007. RepSeq--a database of amino acid repeats present in lower eukaryotic pathogens. BMC Bioinformatics. 8:122.
• Bowyer, P. W., R. S. Gunaratne, M. Grainger, C. Withers-Martinez, S. R. Wickramsinghe, E. W. Tate, R. J. Leatherbarrow, K. A. Brown, A. A. Holder, and D. F. Smith. 2007. Molecules incorporating a benzothiazole core scaffold inhibit the N-myristoyltransferase of Plasmodium falciparum. Biochem J. 408:173-180.
• Tate, E. W., P. W. Bowyer, K. A. Brown, D. F. Smith, and R. J. Leatherbarrow. 2006. Peptide-based inhibitors of N-myristoyl transferase generated from a lipid/combinatorial peptide chimera library. Signal Transduction 6:160-166.
• Prickett, S., P. M. Gray, S. L. Colpitts, P. Scott, P. M. Kaye, and D. F. Smith. 2006. In vivo recognition of ovalbumin expressed by transgenic leishmania is determined by its subcellular localization. J Immunol. 176:4826-4833.
• Polley, R., S. Stager, S. Prickett, A. Maroof, S. Zubairi, D. F. Smith, and P. M. Kaye. 2006. Adoptive immunotherapy against experimental visceral leishmaniasis with CD8+ T cells requires the presence of cognate antigen. Infect Immun. 74:773-776.
• Panethymitaki, C., P. W. Bowyer, H. P. Price, R. J. Leatherbarrow, K. A. Brown, and D. F. Smith. 2006. Characterization and selective inhibition of myristoyl-CoA:protein N-myristoyltransferase from Trypanosoma brucei and Leishmania major. Biochem J. 396:277-285.
• Gray, P. M., S. L. Reiner, D. F. Smith, P. M. Kaye, and P. Scott. 2006. Antigen-Experienced T Cells Limit the Priming of Naive T Cells during Infection with Leishmania major. J Immunol. 177:925-933.
• Denny, P. W., H. Shams-Eldin, H. P. Price, D. F. Smith, and R. T. Schwarz. 2006. The Protozoan Inositol Phosphorylceramide Synthase: a novel drug target that defines a new class of sphingolipid synthase. J Biol Chem. 281:28200-28209.
• Stegmayer, C., A. Kehlenbach, S. Tournaviti, S. Wegehingel, C. Zehe, P. Denny, D. F. Smith, B. Schwappach, and W. Nickel. 2005. Direct transport across the plasma membrane of mammalian cells of Leishmania HASPB as revealed by a CHO export mutant. J Cell Sci 118:517-527.
• Price, H. P., C. Panethymitaki, D. Goulding, and D. F. Smith. 2005. Functional analysis of TbARL1, an N-myristoylated Golgi protein essential for viability in bloodstream trypanosomes. J Cell Sci 118:831-841.
• Price, H. P., D. Goulding, and D. F. Smith. 2005. ARL1 has an essential role in Trypanosoma brucei. Biochem Soc Trans 33:643-645.
• Morgan, G. W., P. W. Denny, S. Vaughan, D. Goulding, T. R. Jeffries, D. F. Smith, K. Gull, and M. C. Field. 2005. An evolutionarily conserved coiled-coil protein implicated in polycystic kidney disease is involved in basal body duplication and flagellar biogenesis in Trypanosoma brucei. Mol Cell Biol 25:3774-3783.
• Ivens, A. C., C. S. Peacock, E. A. Worthey, L. Murphy, G. Aggarwal, M. Berriman, E. Sisk, M. A. Rajandream, E. Adlem, R. Aert, A. Anupama, Z. Apostolou, P. Attipoe, N. Bason, C. Bauser, A. Beck, S. M. Beverley, G. Bianchettin, K. Borzym, G. Bothe, C. V. Bruschi, M. Collins, E. Cadag, L. Ciarloni, C. Clayton, R. M. Coulson, A. Cronin, A. K. Cruz, R. M. Davies, J. De Gaudenzi, D. E. Dobson, A. Duesterhoeft, G. Fazelina, N. Fosker, A. C. Frasch, A. Fraser, M. Fuchs, C. Gabel, A. Goble, A. Goffeau, D. Harris, C. Hertz-Fowler, H. Hilbert, D. Horn, Y. Huang, S. Klages, A. Knights, M. Kube, N. Larke, L. Litvin, A. Lord, T. Louie, M. Marra, D. Masuy, K. Matthews, S. Michaeli, J. C. Mottram, S. Muller-Auer, H. Munden, S. Nelson, H. Norbertczak, K. Oliver, S. O'Neil, M. Pentony, T. M. Pohl, C. Price, B. Purnelle, M. A. Quail, E. Rabbinowitsch, R. Reinhardt, M. Rieger, J. Rinta, J. Robben, L. Robertson, J. C. Ruiz, S. Rutter, D. Saunders, M. Schafer, J. Schein, D. C. Schwartz, K. Seeger, A. Seyler, S. Sharp, H. Shin, D. Sivam, R. Squares, S. Squares, V. Tosato, C. Vogt, G. Volckaert, R. Wambutt, T. Warren, H. Wedler, J. Woodward, S. Zhou, W. Zimmermann, D. F. Smith, J. M. Blackwell, K. D. Stuart, B. Barrell, and P. J. Myler. 2005. The genome of the kinetoplastid parasite, Leishmania major. Science 309:436-442.
• Denny, P. W., G. W. Morgan, M. C. Field, and D. F. Smith. 2005. Leishmania major: clathrin and adaptin complexes of an intra-cellular parasite. Exp Parasitol 109:33-37.
• Ramos, C. S., F. A. Franco, D. F. Smith, and S. R. Uliana. 2004. Characterisation of a new Leishmania META gene and genomic analysis of the META cluster. FEMS Microbiol Lett 238:213-219.
• Nugent, P. G., S. A. Karsani, R. Wait, J. Tempero, and D. F. Smith. 2004. Proteomic analysis of Leishmania mexicana differentiation. Mol Biochem Parasitol 136:51-62.
• Kropf, P., M. A. Freudenberg, M. Modolell, H. P. Price, S. Herath, S. Antoniazi, C. Galanos, D. F. Smith, and I. Muller. 2004. Toll-like receptor 4 contributes to efficient control of infection with the protozoan parasite Leishmania major. Infect Immun 72:1920-1928.
• Denny, P. W., and D. F. Smith. 2004. Rafts and sphingolipid biosynthesis in the kinetoplastid parasitic protozoa. Mol Microbiol 53:725-733.
• Denny, P. W., D. Goulding, M. A. Ferguson, and D. F. Smith. 2004. Sphingolipid-free Leishmania are defective in membrane trafficking, differentiation and infectivity. Mol Microbiol 52:313-327.
• Antoniazi, S., H. P. Price, P. Kropf, M. A. Freudenberg, C. Galanos, D. F. Smith, and I. Muller. 2004. Chemokine gene expression in toll-like receptor-competent and -deficient mice infected with Leishmania major. Infect Immun 72:5168-5174.
• Almeida, R., B. J. Gilmartin, S. H. McCann, A. Norrish, A. C. Ivens, D. Lawson, M. P. Levick, D. F. Smith, S. D. Dyall, D. Vetrie, T. C. Freeman, R. M. Coulson, I. Sampaio, H. Schneider, and J. M. Blackwell. 2004. Expression profiling of the Leishmania life cycle: cDNA arrays identify developmentally regulated genes present but not annotated in the genome. Mol Biochem Parasitol 136:87-100.
• Zufferey, R., S. Allen, T. Barron, D. R. Sullivan, P. W. Denny, I. C. Almeida, D. F. Smith, S. J. Turco, M. A. Ferguson, and S. M. Beverley. 2003. Ether phospholipids and glycosylinositolphospholipids are not required for amastigote virulence or for inhibition of macrophage activation by Leishmania major. J Biol Chem 278:44708-44718.
• Stager, S., J. Alexander, A. C. Kirby, M. Botto, N. V. Rooijen, D. F. Smith, F. Brombacher, and P. M. Kaye. 2003. Natural antibodies and complement are endogenous adjuvants for vaccine-induced CD8(+) T-cell responses. Nat Med 9:1287-1292.
• Price, H. P., M. R. Menon, C. Panethymitaki, D. Goulding, P. G. McKean, and D. F. Smith. 2003. Myristoyl-CoA:Protein N-Myristoyltransferase, an Essential Enzyme and Potential Drug Target in Kinetoplastid Parasites. J Biol Chem 278:7206-7214.
• Gelb, M. H., W. C. Van Voorhis, F. S. Buckner, K. Yokoyama, R. Eastman, E. P. Carpenter, C. Panethymitaki, K. A. Brown, and D. F. Smith. 2003. Protein farnesyl and N-myristoyl transferases: piggy-back medicinal chemistry targets for the development of antitrypanosomatid and antimalarial therapeutics. Molecular and Biochemical Parasitology 126:155-163.
• Denny, P. W., S. Lewis, J. E. Tempero, D. Goulding, A. C. Ivens, M. C. Field, and D. F. Smith. 2002. Leishmania RAB7: characterisation of terminal endocytic stages in an intracellular parasite. Mol Biochem Parasitol 123:105-113.
• McKean, P. G., J. K. Keen, D. F. Smith, and F. E. Benson. 2001. Identification and characterisation of a RAD51 gene from Leishmania major. Mol Biochem Parasitol 115:209-216.
• McKean, P. G., P. W. Denny, E. Knuepfer, J. K. Keen, and D. F. Smith. 2001. Phenotypic changes associated with deletion and overexpression of a stage-regulated gene family in Leishmania. Cell Microbiol 3:511-523.
• Kuratli, S., A. Hemphill, J. Lindh, D. F. Smith, and B. Connolly. 2001. Secretion of the novel Trichinella protein TSJ5 by T. spiralis and T. pseudospiralis muscle larvae. Mol Biochem Parasitol 115:199-208.
• Knuepfer, E., Y. D. Stierhof, P. G. McKean, and D. F. Smith. 2001. Characterization of a differentially expressed protein that shows an unusual localization to intracellular membranes in Leishmania major. Biochem J 356:335-344.
• Denny, P. W., M. C. Field, and D. F. Smith. 2001. GPI-anchored proteins and glycoconjugates segregate into lipid rafts in Kinetoplastida. FEBS Lett 491:148-153.
• Stager, S., D. F. Smith, and P. M. Kaye. 2000. Immunization with a recombinant stage-regulated surface protein from Leishmania donovani induces protection against visceral leishmaniasis. J Immunol 165:7064-7071.
• Ma, S. 2000. Identification and characterisation of HASPB homologues in New World Leishmania species. PhD thesis, University of London.
• Dyall, S. D., P. W. Denny, R. Seepersaud, D. McGhie, and D. F. Smith. 2000. Expression of the AM gene locus in infective stages of Leishmania. Mol Biochem Parasitol 109:73-79.
• Denny, P. W., S. Gokool, D. G. Russell, M. C. Field, and D. F. Smith. 2000. Acylation-dependent protein export in Leishmania. J Biol Chem 275:11017-11025.
• Bates, E. J., E. Knuepfer, and D. F. Smith. 2000. Poly(A)-binding protein I of Leishmania: functional analysis and localisation in trypanosomatid parasites. Nucleic Acids Res 28:1211-1220.
• Uliana, S. R., N. Goyal, E. Freymuller, and D. F. Smith. 1999. Leishmania: overexpression and comparative structural analysis of the stage-regulated meta 1 gene. Exp Parasitol 92:183-191.
• McKean, P. G., J. K. Keen, J. M. Kelly, and D. F. Smith. 1999. Molecular Parasitology: New Insights. Parasitology Today 15:469-470.
• Kuratli, S., J. G. Lindh, B. Gottstein, D. F. Smith, and B. Connolly. 1999. Trichinella spp.: differential expression of two genes in the muscle larva of encapsulating and nonencapsulating species. Exp Parasitol 93:153-159.
• Jensen, A. T., S. Gasim, T. Moller, A. Ismail, A. Gaafar, M. Kemp, A. M. el Hassan, A. Kharazmi, T. M. Alce, D. F. Smith, and T. G. Theander. 1999. Serodiagnosis of Leishmania donovani infections: assessment of enzyme-linked immunosorbent assays using recombinant L. donovani gene B protein (GBP) and a peptide sequence of L. donovani GBP. Trans R Soc Trop Med Hyg 93:157-160.
• Alce, T. M., S. Gokool, D. McGhie, S. Stager, and D. F. Smith. 1999. Expression of hydrophilic surface proteins in infective stages of Leishmania donovani. Mol Biochem Parasitol 102:191-196.
• Lindh, J. G., B. Connolly, D. L. McGhie, and D. F. Smith. 1998. Identification of a developmentally regulated Trichinella spiralis protein that inhibits MyoD-specific protein: DNA complexes in vitro. Mol Biochem Parasitol 92:163-175.
• Ivens, A. C., S. M. Lewis, A. Bagherzadeh, L. Zhang, H. M. Chan, and D. F. Smith. 1998. A physical map of the Leishmania major Friedlin genome. Genome Res 8:135-145.
• Clayton, C., M. Adams, R. Almeida, T. Baltz, M. Barrett, P. Bastien, S. Belli, S. Beverley, N. Biteau, J. Blackwell, C. Blaineau, M. Boshart, F. Bringaud, G. Cross, A. Cruz, W. Degrave, J. Donelson, N. El-Sayed, G. Fu, K. Ersfeld, W. Gibson, K. Gull, A. Ivens, J. Kelly, L. Vanhamme, and et al. 1998. Genetic nomenclature for Trypanosoma and Leishmania. Mol Biochem Parasitol 97:221-224.
• McKean, P. G., K. R. Trenholme, D. Rangarajan, J. K. Keen, and D. F. Smith. 1997. Diversity in repeat-containing surface proteins of Leishmania major. Mol Biochem Parasitol 86:225-235.
• McKean, P. G., R. Delahay, P. F. Pimenta, and D. F. Smith. 1997. Characterisation of a second protein encoded by the differentially regulated LmcDNA16 gene family of Leishmania major. Mol Biochem Parasitol 85:221-231.
• Ivens, A. C., and D. F. Smith. 1997. Parasite genome analysis. A global map of the Leishmania major genome: prelude to genomic sequencing. Trans R Soc Trop Med Hyg 91:111-115.
• Nourbakhsh, F., S. R. Uliana, and D. F. Smith. 1996. Characterisation and expression of a stage-regulated gene of Leishmania major. Mol Biochem Parasitol 76:201-213.
• Connolly, B., K. Trenholme, and D. F. Smith. 1996. Molecular cloning of a myoD-like gene from the parasitic nematode, Trichinella spiralis. Mol Biochem Parasitol 81:137-149.
• Booth, D. R., P. D. Ready, and D. F. Smith. 1996. Evolution of multiple families of non-LTR retrotransposons in phlebotomine sandflies. Genet Res 67:227-237.
• Smith, D. F., and D. Rangarajan. 1995. Cell surface components of Leishmania: identification of a novel parasite lectin? Glycobiology 5:161-166.
• Rangarajan, D., S. Gokool, M. V. McCrossan, and D. F. Smith. 1995. The gene B protein localises to the surface of Leishmania major parasites in the absence of metacyclic stage lipophosphoglycan. J Cell Sci 108 ( Pt 11):3359-3366.
• Kelly, B. L., S. D. Dyall, J. P. Warner, J. Tang, and D. F. Smith. 1995. Chromosomal organisation of a repeated gene cluster expressed in mammalian stages of Leishmania. Gene 163:145-149.
• Connolly, B., L. J. Ingram, and D. F. Smith. 1995. Trichinella spiralis: cloning and characterisation of two repetitive DNA sequences. Exp Parasitol 80:488-498.
• Smith, D. F., S. Gokool, J. K. Keen, P. G. McKean, and D. Rangarajan. 1994. Structure and function of infective stage proteins of Leishmania. Biochem Soc Trans 22:286-291.
• Pimenta, P. F., P. Pinto da Silva, D. Rangarajan, D. F. Smith, and D. L. Sacks. 1994. Leishmania major: association of the differentially expressed gene B protein and the surface lipophosphoglycan as revealed by membrane capping. Exp Parasitol 79:468-479.
• Louzir, H., F. Tebourski, D. F. Smith, R. B. Ismail, and K. Dellagi. 1994. Antibodies to Leishmania donovani infantum heat-shock protein 70 in human visceral leishmaniasis. J Infect Dis 169:1183-1184.
• Flinn, H. M., D. Rangarajan, and D. F. Smith. 1994. Expression of a hydrophilic surface protein in infective stages of Leishmania major. Mol Biochem Parasitol 65:259-270.
• Booth, D. R., P. D. Ready, and D. F. Smith. 1994. Isolation of non-LTR retrotransposon reverse transcriptase-like sequences from phlebotomine sandflies. Insect Mol Biol 3:89-96.
• Searle, S., and D. F. Smith. 1993. Leishmania major: characterisation and expression of a cytoplasmic stress-related protein. Exp Parasitol 77:43-52.
• Searle, S., M. V. McCrossan, and D. F. Smith. 1993. Expression of a mitochondrial stress protein in the protozoan parasite Leishmania major. J Cell Sci 104 ( Pt 4):1091-1100.
• Maizels, R. M., D. A. Bundy, M. E. Selkirk, D. F. Smith, and R. M. Anderson. 1993. Immunological modulation and evasion by helminth parasites in human populations. Nature 365:797-805.
• Gokool, S., C. F. Curtis, and D. F. Smith. 1993. Analysis of mosquito bloodmeals by DNA profiling. Med Vet Entomol 7:208-215.
• Gramiccia, M., D. F. Smith, M. C. Angelici, P. D. Ready, and L. Gradoni. 1992. A kinetoplast DNA probe diagnostic for Leishmania infantum. Parasitology 105 ( Pt 1):29-34.
• Gokool, S., D. F. Smith, and C. F. Curtis. 1992. The use of PCR to help quantify the protection provided by impregnated bednets. Parasitology Today 8:347- 350.
• Flinn, H. M., and D. F. Smith. 1992. Genomic organisation and expression of a differentially-regulated gene family from Leishmania major. Nucleic Acids Res 20:755-762.
• Ben-Ismail, R., D. F. Smith, P. D. Ready, A. Ayadi, M. Gramiccia, A. Ben-Osman, and M. S. Ben-Rachid. 1992. Sporadic cutaneous leishmaniasis in north Tunisia: identification of the causative agent as Leishmania infantum by the use of a diagnostic deoxyribonucleic acid probe. Trans R Soc Trop Med Hyg 86:508-510.
• Roberts, D. B., T. Jowett, J. Hughes, D. F. Smith, and D. M. Glover. 1991. The major serum protein of Drosophila larvae, larval serum protein 1, is dispensable. Eur J Biochem 195:195-201.
• Booth, D. R., P. D. Ready, and D. F. Smith. 1991. Retrotransposons and evolution in phlebotomines. Parassitologia 33:105-112.
• Smith, D. F. 1990. Trypanosomatid transfection: stable introduction of DNA into protozoa. Parasitology Today 6:245-247.
• Coulson, R. M., and D. F. Smith. 1990. Isolation of genes showing increased or unique expression in the infective promastigotes of Leishmania major. Mol Biochem Parasitol 40:63-75.
• Coulson, R. M., C. F. Curtis, P. D. Ready, N. Hill, and D. F. Smith. 1990. Amplification and analysis of human DNA present in mosquito bloodmeals. Med Vet Entomol 4:357-366.
• Smith, D. F., S. Searle, P. D. Ready, M. Gramiccia, and R. Ben-Ismail. 1989. A kinetoplast DNA probe diagnostic for Leishmania major: sequence homologies between regions of Leishmania minicircles. Mol Biochem Parasitol 37:213-223.
• Searle, S., A. J. Campos, R. M. Coulson, T. W. Spithill, and D. F. Smith. 1989. A family of heat shock protein 70-related genes are expressed in the promastigotes of Leishmania major. Nucleic Acids Res 17:5081-5095.
• Ready, P. D., D. F. Smith, and R. Killick-Kendrick. 1988. DNA hybridizations on squash-blotted sandflies to identify both Phlebotomus papatasi and infecting Leishmania major. Med Vet Entomol 2:109-116.
• Ready, P. D., and D. F. Smith. 1988. Peanut lectin agglutination and isolation of infective forms of Leishmania major. Trans R Soc Trop Med Hyg 82:418.
• Maroli, M., M. Gramiccia, L. Gradoni, P. D. Ready, D. F. Smith, and C. Aquino. 1988. Natural infections of phlebotomine sandflies with Trypanosomatidae in central and south Italy. Trans R Soc Trop Med Hyg 82:227-228.
• Ready, P. D., R. Killick-Kendrick, D. F. Smith, and M. Bailly. 1986. rDNA structural polymorphisms as diagnostic markers for populations of Phlebotomus papatasi from areas endemic and non-endemic for zoonotic cutaneous leishmaniasis. Trans R Soc Trop Med Hyg 80:341-342.
• Delaney, S. J., D. F. Smith, A. McClelland, C. Sunkel, and D. M. Glover. 1986. Sequence conservation around the 5' ends of the larval serum protein 1 genes of Drosophila melanogaster. J Mol Biol 189:1-11.
• Smith, D. F., A. McClelland, B. N. White, C. F. Addison, and D. M. Glover. 1981. The molecular cloning of a dispersed set of developmentally regulated genes which encode the major larval serum protein of D. melanogaster. Cell 23:441-449.
• McClelland, A., D. F. Smith, and D. M. Glover. 1981. Short intervening sequences close to the 5' ends of the three Drosophila larval serum protein 1 genes. J Mol Biol 153:257-272.
• Tata, J. R., and D. F. Smith. 1979. Vitellogenesis: a versatile model for hormonal regulation of gene expression. Recent Prog Horm Res 35:47-95.
• Smith, D. F., P. F. Searle, and J. G. Williams. 1979. Characterisation of bacterial clones containing DNA sequences derived from Xenopus laevis vitellogenin mRNA. Nucleic Acids Res 6:487-506.
• Smith, D. F., T. M. Penning, A. Q. Ansari, K. A. Munday, and M. Akhtar. 1978. Oestrogen-induced cholesterol and fatty acid biosynthesis in Xenopus laevis liver during vitellogenic response. Biochem J 174:353-361.

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• Editorial review board: Microbes and Infection