The Cold War in Mitosis: Mutual Antagonism between the Spindle Assembly Checkpoint and the APC/C

Monday 5 November 2012, 1.15PM

Speaker: Dr Jon Pines, University of Cambridge

 Abstract

We are trying to understand how cells divide to ensure that their two daughter cells receive an identical copy of the genome. The key to the control of chromosome segregation is the regulation of the Anaphase Promoting Complex/Cyclosome (APC/C) by the spindle assembly checkpoint. We have developed a live cell assay to monitor APC/C activity and its inhibition by the spindle checkpoint. We have refined this assay by using somatic cell gene-targeting to generate cell lines in which one allele of the gene for an APC/C substrate or SAC protein is tagged with a fluorescent protein. We are using these recombinant cell lines in a quantitative analysis of the SAC in vivo under different conditions.

Host: Dawn Coverley

Location: Biology Lecture Theatre (K018)

Email: cii@york.ac.uk

Telephone: 01904 328876